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Effects of Modulating the Culture Mi...

Liang, Zhiyu.

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Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells./
Author:	Liang, Zhiyu.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
Description:	115 p.
Notes:	Source: Masters Abstracts International, Volume: 84-10.
Contained By:	Masters Abstracts International84-10.
Subject:	Tumor necrosis factor-TNF. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30360939
ISBN:	9798377679912

Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells.
Liang, Zhiyu.

Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 115 p.

Source: Masters Abstracts International, Volume: 84-10.

Thesis (M.E.)--The University of Western Ontario (Canada), 2022.

The cell microenvironment plays a critical role in modulating adipose-derived stromal cell (ASC) proliferation and paracrine function. The current study investigated the effects of decellularized adipose tissue (DAT) coatings, low-level oscillatory shear stress (~0.04‑0.3 dyn/cm2), hypoxia (2% O2), and pro-inflammatory cytokine priming with IFN-g and TNF-a on human ASC proliferation and paracrine factor secretion in the context of a rocking bioreactor. Culturing under 20% O2 resulted in a higher cell density after 7 days of culture. Without cytokine priming, the varying culture conditions significantly impacted the levels of the pro-angiogenic factors VEGF, HGF, and angiogenin detected in conditioned media samples. In contrast, when the cells were primed, the levels of the immunomodulatory factors IL-6 and IL-8 were most affected by the varying microenvironmental factors. Overall, a novel bioreactor system was developed for ASC expansion and preconditioning, demonstrating that the cell microenvironment could be tuned to modulate ASC paracrine factor secretion.

ISBN: 9798377679912Subjects--Topical Terms:

3560383
Tumor necrosis factor-TNF.

Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells.
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