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Synthesis and Characterization of DN...

Lee, Jung Kyu.

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Synthesis and Characterization of DNA-Single Molecule-DNA Triblock Structures: As a Novel Approach Towards Single-Molecule Electronics.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Synthesis and Characterization of DNA-Single Molecule-DNA Triblock Structures: As a Novel Approach Towards Single-Molecule Electronics./
作者:	Lee, Jung Kyu.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2010,
面頁冊數:	140 p.
附註:	Source: Dissertations Abstracts International, Volume: 82-03, Section: B.
Contained By:	Dissertations Abstracts International82-03B.
標題:	Chemistry. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28169099
ISBN:	9798672114972

Synthesis and Characterization of DNA-Single Molecule-DNA Triblock Structures: As a Novel Approach Towards Single-Molecule Electronics.
Lee, Jung Kyu.

Synthesis and Characterization of DNA-Single Molecule-DNA Triblock Structures: As a Novel Approach Towards Single-Molecule Electronics. - Ann Arbor : ProQuest Dissertations & Theses, 2010 - 140 p.

Source: Dissertations Abstracts International, Volume: 82-03, Section: B.

Thesis (Ph.D.)--Stanford University, 2010.

A precise and reproducible electrical contact between a single molecule and the electrodes is the first step in studying single-molecule electronics, which uses individual molecules as active electronic components. One potentially promising strategy to make the electrical contact to a single molecule is to use DNA as a template. DNA has emerged as a good scaffold in the field of nanoelectronics because DNA is easily aligned over large areas, and can be employed as a conducting nanowire with micrometer-scaled length after metallization using metal ions. Moreover, oligodeoxynucleotide (ODN) can be readily linked to a single organic molecule, and its length can be further increased to several micrometer scales through DNA extension techniques.To build DNA-assist single-molecule device structures, I investigated the reactivity of ODN to synthesize organic molecule-bis(ODN) triblock oligomers through three separate cross-coupling routes, such as amide-coupling reaction, isothiourea-bond formation, and "click" chemistry. Specifically, the amide-coupling reaction is scrutinized to enhance its reactivity since it affords the highest yield among the cross-coupling reactions. The optimized amide-coupling reaction is also employed to incorporate functional organic molecules, involving a fluorophore and a conjugated polymer, into ODNs. Organic molecule-bis(ODN) triblock oligomers were characterized by denaturing gel electrophoresis and electrospray ionization mass spectrometry.The ODNs of the triblock oligomers are elongated by polymerase chain reaction (PCR) or DNA hybridization/ligation methods. PCR is a fast and precise method to construct organic molecule-bis(1.5 kbp dsDNA) triblock structures from the triblock oligomers. On the other hand, DNA hybridization/ligation affords longer length of the ODN using micrometer-sized DNA fragments, which are prepared from lambda DNA using restriction enzymes and a phosphatase. Thus, organic molecule-bis(micrometer-sized DNA) triblock structures are assembled to obtain fully stretched DNA strands. To characterize the triblock structures, fluorophore-bis(micrometer-sized DNA) triblock structure was synthesized through DNA hybridization/ligation, and then directly imaged by combined atomic force and single-molecule fluorescence microscopy.For the purpose of building a single-molecule transistor device, a conjugated polymer-bis(micrometer-sized DNA) triblock structure was metallized by palladium metal ion. The metallized triblock structure is characterized by scanning electron microscopy (SEM) to monitor a nanogap from the conjugated polymer (contour length: ~7 nm). Unfortunately, the nanogap is not observed, due to overgrowth of metal ions during the DNA metallization. To overcome the problem, I also describe the synthesis of a micrometer-sized DNA-conjugated polymer-gold nanoparticle asymmetric triblock structure.

ISBN: 9798672114972Subjects--Topical Terms:

516420
Chemistry.
Subjects--Index Terms:

Single-molecule transistor device

Synthesis and Characterization of DNA-Single Molecule-DNA Triblock Structures: As a Novel Approach Towards Single-Molecule Electronics.
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