東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

CRISPR'S Gambit: Novel tactics for g...

Trasanidou, Despoina.

FindBook

Google Book

Amazon

博客來

CRISPR'S Gambit: Novel tactics for genetic engineering of human and bacterial cells.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	CRISPR'S Gambit: Novel tactics for genetic engineering of human and bacterial cells./
作者:	Trasanidou, Despoina.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	520 p.
附註:	Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
Contained By:	Dissertations Abstracts International85-12B.
標題:	Plasmids. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31472636
ISBN:	9798382828800

CRISPR'S Gambit: Novel tactics for genetic engineering of human and bacterial cells.
Trasanidou, Despoina.

CRISPR'S Gambit: Novel tactics for genetic engineering of human and bacterial cells. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 520 p.

Source: Dissertations Abstracts International, Volume: 85-12, Section: B.

Thesis (Ph.D.)--Wageningen University and Research, 2023.

Languishing in the quagmire of food shortage and devastating diseases, humanity has always been pursuing novel ways to improve the quality of life. As described in Chapter 1, the millennia-old practices of domestication and selective breeding of agricultural plants and animals were first subjected to the magnifying glass of scientists around 240 years ago. Studying the evolution and genetics of wild fauna and flora, the restless spirit of humankind quickly led to the first attempts of direct but random genetic intervention, which were followed by the discovery and manipulation of the molecule of life (DNA). Around 30 years ago, the first genetically modified organisms were commercialized, and, shortly after, the sequencing technology gave rise to the development of primary tools for site-directed genomic modification. However, these tools were inefficient, required selectable markers, and were leaving genomic scars. Hence, efficient and programmable endonucleases (meganucleases, Zinc Finger Nucleases, and TALE Nucleases) entered the scene, though necessitating laborious, expensive, and time-consuming protein engineering. The discovery, characterization and repurposing of CRISPR-Cas systems during the last two decades have revolutionised genetic engineering, providing simple, cost-effective, and quick methods for site-specific genome editing and transcriptional regulation. CRISPR-Cas is a highly diverse set of prokaryotic adaptive immune systems against viruses, plasmids, and conjugative elements. Upon recognition and cleavage of foreign genetic material, a short DNA fragment is integrated into the CRISPR array (adaptation), forming immunological memory. The CRISPR array is transcribed into a long pre-crRNA molecule that is processed into short, mature crRNAs (expression) that guide Cas nucleases to target and cleave the DNA or RNA of cognate invaders. CRISPR-Cas systems are classified into two classes, six types and several subtypes. Due to their widespread presence and the eternal evolutionary arms race between cellular host organisms and their MGE invaders, CRISPR-Cas systems exhibit an impressive plethora and variety, part of which has been harnessed for genetic engineering purposes. As such, genome editing is performed using DNA nucleases, DNA base-editors, prime-editors, and Cas-related transposases or recombinases. Transcriptional regulation takes place through Cas-related transcriptional effector domains, RNA nucleases, and RNA base-editors. The excessive activity of CRISPR-Cas tools is controlled via ligand-inducible expression, complementary oligonucleotides, and virus-encoded anti-CRISPR proteins. CRISPR-Cas technology currently holds the reins of genetic engineering, being constantly optimised for safe and efficient applications in gene therapy, crop improvement, and industrial strain development.

ISBN: 9798382828800Subjects--Topical Terms:

3319070
Plasmids.

CRISPR'S Gambit: Novel tactics for genetic engineering of human and bacterial cells.
LDR:03925nmm a2200337 4500 001 2399516
005 20240916065505.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798382828800
035 $a (MiAaPQ)AAI31472636
035 $a (MiAaPQ)Wageningen614393
035 $a AAI31472636
040 $a MiAaPQ $c MiAaPQ
100 1 $a Trasanidou, Despoina. $3 3769489
245 1 0 $a CRISPR'S Gambit: Novel tactics for genetic engineering of human and bacterial cells.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 520 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
500 $a Advisor: van der Oost, John;Staals, Raymond.
502 $a Thesis (Ph.D.)--Wageningen University and Research, 2023.
520 $a Languishing in the quagmire of food shortage and devastating diseases, humanity has always been pursuing novel ways to improve the quality of life. As described in Chapter 1, the millennia-old practices of domestication and selective breeding of agricultural plants and animals were first subjected to the magnifying glass of scientists around 240 years ago. Studying the evolution and genetics of wild fauna and flora, the restless spirit of humankind quickly led to the first attempts of direct but random genetic intervention, which were followed by the discovery and manipulation of the molecule of life (DNA). Around 30 years ago, the first genetically modified organisms were commercialized, and, shortly after, the sequencing technology gave rise to the development of primary tools for site-directed genomic modification. However, these tools were inefficient, required selectable markers, and were leaving genomic scars. Hence, efficient and programmable endonucleases (meganucleases, Zinc Finger Nucleases, and TALE Nucleases) entered the scene, though necessitating laborious, expensive, and time-consuming protein engineering. The discovery, characterization and repurposing of CRISPR-Cas systems during the last two decades have revolutionised genetic engineering, providing simple, cost-effective, and quick methods for site-specific genome editing and transcriptional regulation. CRISPR-Cas is a highly diverse set of prokaryotic adaptive immune systems against viruses, plasmids, and conjugative elements. Upon recognition and cleavage of foreign genetic material, a short DNA fragment is integrated into the CRISPR array (adaptation), forming immunological memory. The CRISPR array is transcribed into a long pre-crRNA molecule that is processed into short, mature crRNAs (expression) that guide Cas nucleases to target and cleave the DNA or RNA of cognate invaders. CRISPR-Cas systems are classified into two classes, six types and several subtypes. Due to their widespread presence and the eternal evolutionary arms race between cellular host organisms and their MGE invaders, CRISPR-Cas systems exhibit an impressive plethora and variety, part of which has been harnessed for genetic engineering purposes. As such, genome editing is performed using DNA nucleases, DNA base-editors, prime-editors, and Cas-related transposases or recombinases. Transcriptional regulation takes place through Cas-related transcriptional effector domains, RNA nucleases, and RNA base-editors. The excessive activity of CRISPR-Cas tools is controlled via ligand-inducible expression, complementary oligonucleotides, and virus-encoded anti-CRISPR proteins. CRISPR-Cas technology currently holds the reins of genetic engineering, being constantly optimised for safe and efficient applications in gene therapy, crop improvement, and industrial strain development.
590 $a School code: 2157.
650 4 $a Plasmids. $3 3319070
650 4 $a RNA polymerase. $3 3561726
650 4 $a CRISPR. $3 3561750
650 4 $a Gene expression. $3 643979
650 4 $a Mutation. $3 837917
650 4 $a Bacteria. $3 550366
650 4 $a Genetic engineering. $3 530509
650 4 $a Adaptation. $3 3562958
650 4 $a Viruses. $3 571474
650 4 $a Genomes. $3 592593
650 4 $a Adaptive immunity. $3 3691695
650 4 $a Enzymes. $3 520899
650 4 $a Kinases. $3 3558077
650 4 $a Cell cycle. $3 766119
650 4 $a Proteins. $3 558769
650 4 $a Bioinformatics. $3 553671
650 4 $a Biochemistry. $3 518028
650 4 $a Genetics. $3 530508
690 $a 0487
690 $a 0369
690 $a 0715
710 2 $a Wageningen University and Research. $3 3557914
773 0 $t Dissertations Abstracts International $g 85-12B.
790 $a 2157
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31472636