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In Silico Identification, Characteri...

Cupp, Victor.

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In Silico Identification, Characterization, and In Vitro Expression of Phage Endolysins From Streptomyces Spp. in BL21(DE3) Escherichia coli.

Record Type:	Electronic resources : Monograph/item
Title/Author:	In Silico Identification, Characterization, and In Vitro Expression of Phage Endolysins From Streptomyces Spp. in BL21(DE3) Escherichia coli./
Author:	Cupp, Victor.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
Description:	60 p.
Notes:	Source: Masters Abstracts International, Volume: 85-04.
Contained By:	Masters Abstracts International85-04.
Subject:	Plant sciences. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30690381
ISBN:	9798380605571

In Silico Identification, Characterization, and In Vitro Expression of Phage Endolysins From Streptomyces Spp. in BL21(DE3) Escherichia coli.
Cupp, Victor.

In Silico Identification, Characterization, and In Vitro Expression of Phage Endolysins From Streptomyces Spp. in BL21(DE3) Escherichia coli. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 60 p.

Source: Masters Abstracts International, Volume: 85-04.

Thesis (M.Agr.Sc.)--Florida Agricultural and Mechanical University, 2023.

This item must not be sold to any third party vendors.

Potato is the sixth largest crop harvested in the United States. Potato diseases cost billions of dollars to the US and worldwide potato industry. Continuous reports on soilborne diseases and the increasing prevalence of antibiotic-resistant bacteria call for developing novel techniques that decrease disease while assuring the environment's safety. Bacteriophage endolysins are highly active antibacterial peptidoglycan hydrolases that have evolved over millions of years to become the ultimate weapon against bacteria, potentially used as therapeutics. Among potatoes' significant soilborne bacterial diseases is common scab, a Gram-positive Streptomyces spp. There are no efficacious pesticides or soil fumigants for treating this disease. In this study, we used bioinformatics to screen large databases and identified seven endolysin proteins from Streptomyces spp. These endolysins are promising candidates that could be used to eliminate common scab bacterial pathogens in potatoes specifically. The seven endolysin proteins identified are: AYD86781.1 LysM-like endolysin Streptomyces phage Microdon; QPL14064.1 endolysin Streptomyces phage TurkishDelight; AWN05104.1 endolysin Streptomyces phage FlowerPower; YP 009615350.1 endolysin Streptomyces phage Scap1; AWN05870.1 hydrolase Streptomyces phage Rainydai; QJD53688.1 endolysin Streptomyces phage Kela; and QAX92944.1 LysM-like endolysin Streptomyces phage WheeHeim. These are phage lytic proteins, an advanced class of novel enzyme-based antibiotics. A growing community of researchers develops phage lytic proteins with the perspective of their use as antibiotics.

ISBN: 9798380605571Subjects--Topical Terms:

3173832
Plant sciences.
Subjects--Index Terms:

Bacteria

In Silico Identification, Characterization, and In Vitro Expression of Phage Endolysins From Streptomyces Spp. in BL21(DE3) Escherichia coli.
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245 1 0 $a In Silico Identification, Characterization, and In Vitro Expression of Phage Endolysins From Streptomyces Spp. in BL21(DE3) Escherichia coli.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 60 p.
500 $a Source: Masters Abstracts International, Volume: 85-04.
500 $a Advisor: Tsolova, Violeta M.
502 $a Thesis (M.Agr.Sc.)--Florida Agricultural and Mechanical University, 2023.
506 $a This item must not be sold to any third party vendors.
520 $a Potato is the sixth largest crop harvested in the United States. Potato diseases cost billions of dollars to the US and worldwide potato industry. Continuous reports on soilborne diseases and the increasing prevalence of antibiotic-resistant bacteria call for developing novel techniques that decrease disease while assuring the environment's safety. Bacteriophage endolysins are highly active antibacterial peptidoglycan hydrolases that have evolved over millions of years to become the ultimate weapon against bacteria, potentially used as therapeutics. Among potatoes' significant soilborne bacterial diseases is common scab, a Gram-positive Streptomyces spp. There are no efficacious pesticides or soil fumigants for treating this disease. In this study, we used bioinformatics to screen large databases and identified seven endolysin proteins from Streptomyces spp. These endolysins are promising candidates that could be used to eliminate common scab bacterial pathogens in potatoes specifically. The seven endolysin proteins identified are: AYD86781.1 LysM-like endolysin Streptomyces phage Microdon; QPL14064.1 endolysin Streptomyces phage TurkishDelight; AWN05104.1 endolysin Streptomyces phage FlowerPower; YP 009615350.1 endolysin Streptomyces phage Scap1; AWN05870.1 hydrolase Streptomyces phage Rainydai; QJD53688.1 endolysin Streptomyces phage Kela; and QAX92944.1 LysM-like endolysin Streptomyces phage WheeHeim. These are phage lytic proteins, an advanced class of novel enzyme-based antibiotics. A growing community of researchers develops phage lytic proteins with the perspective of their use as antibiotics.
590 $a School code: 0872.
650 4 $a Plant sciences. $3 3173832
650 4 $a Bioinformatics. $3 553671
650 4 $a Microbiology. $3 536250
653 $a Bacteria
653 $a Endolysin
653 $a Streptomyces
653 $a Proteins
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690 $a 0715
690 $a 0410
710 2 $a Florida Agricultural and Mechanical University. $b Division of Agricultural Sciences. $3 3547021
773 0 $t Masters Abstracts International $g 85-04.
790 $a 0872
791 $a M.Agr.Sc.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30690381