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Development of Selective Fluorescent...

Gilyot, Glenn D., Jr.

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Development of Selective Fluorescent Sensors for Detection of Biologically Relevant Analytes.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development of Selective Fluorescent Sensors for Detection of Biologically Relevant Analytes./
Author:	Gilyot, Glenn D., Jr.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
Description:	125 p.
Notes:	Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
Contained By:	Dissertations Abstracts International85-04B.
Subject:	Organic chemistry. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30571521
ISBN:	9798380617697

Development of Selective Fluorescent Sensors for Detection of Biologically Relevant Analytes.
Gilyot, Glenn D., Jr.

Development of Selective Fluorescent Sensors for Detection of Biologically Relevant Analytes. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 125 p.

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

Thesis (Ph.D.)--University of Missouri - Columbia, 2023.

This item must not be sold to any third party vendors.

Fluorescent sensing has become an important tool in the detection and monitoring of biological materials. Specifically, fluorescent sensing has allowed us to make inroads into understanding the complex roles of proteins and peptides, as well as imaging cellular structures and tumors. Herein, we propose three small molecule fluorescent sensing platforms for the recognition of certain biologically relevant analytes.First, we developed a near-infrared (NIR), near-neutral cyanine dye for the detection of pH changes in vivo. Our results indicated our dye was able to detect changes in pH with a pKa in the near-neutral range. In addition, the dye was successfully encapsulated in red blood cells through low hemoglobin ghosting for in vivo measurement of pH with pKa values comparable to the in vitro studies.Second, we developed a small molecule coumarin-based fluorogenic probe for protein detection. As a proof of concept, we modeled this probe with a biotin-PEG3 moiety for detection of streptavidin. Our results indicated a preferential binding with significant changes in fluorescence compared to glycine (Gly), lysine (Lys) and bovine serum albumin (BSA).

ISBN: 9798380617697Subjects--Topical Terms:

523952
Organic chemistry.
Subjects--Index Terms:

Biological materials

Development of Selective Fluorescent Sensors for Detection of Biologically Relevant Analytes.
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500 $a Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
500 $a Advisor: Glass, Timothy.
502 $a Thesis (Ph.D.)--University of Missouri - Columbia, 2023.
506 $a This item must not be sold to any third party vendors.
520 $a Fluorescent sensing has become an important tool in the detection and monitoring of biological materials. Specifically, fluorescent sensing has allowed us to make inroads into understanding the complex roles of proteins and peptides, as well as imaging cellular structures and tumors. Herein, we propose three small molecule fluorescent sensing platforms for the recognition of certain biologically relevant analytes.First, we developed a near-infrared (NIR), near-neutral cyanine dye for the detection of pH changes in vivo. Our results indicated our dye was able to detect changes in pH with a pKa in the near-neutral range. In addition, the dye was successfully encapsulated in red blood cells through low hemoglobin ghosting for in vivo measurement of pH with pKa values comparable to the in vitro studies.Second, we developed a small molecule coumarin-based fluorogenic probe for protein detection. As a proof of concept, we modeled this probe with a biotin-PEG3 moiety for detection of streptavidin. Our results indicated a preferential binding with significant changes in fluorescence compared to glycine (Gly), lysine (Lys) and bovine serum albumin (BSA).
590 $a School code: 0133.
650 4 $a Organic chemistry. $3 523952
650 4 $a Biomedical engineering. $3 535387
650 4 $a Biochemistry. $3 518028
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653 $a Fluorescent sensing
653 $a Bovine serum albumin
653 $a Fluorogenic probe
653 $a Red blood cells
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