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Examining the Gene Expression Profil...

Leow, Chi Jing.

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Examining the Gene Expression Profiles of Killifishes With Different Life-Histories Using Liver and Brain Tissues (Cyprinodontiformes: Nothobranchiidae).

Record Type:	Electronic resources : Monograph/item
Title/Author:	Examining the Gene Expression Profiles of Killifishes With Different Life-Histories Using Liver and Brain Tissues (Cyprinodontiformes: Nothobranchiidae)./
Author:	Leow, Chi Jing.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
Description:	101 p.
Notes:	Source: Masters Abstracts International, Volume: 85-01.
Contained By:	Masters Abstracts International85-01.
Subject:	Biology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30569503
ISBN:	9798379896683

Examining the Gene Expression Profiles of Killifishes With Different Life-Histories Using Liver and Brain Tissues (Cyprinodontiformes: Nothobranchiidae).
Leow, Chi Jing.

Examining the Gene Expression Profiles of Killifishes With Different Life-Histories Using Liver and Brain Tissues (Cyprinodontiformes: Nothobranchiidae). - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 101 p.

Source: Masters Abstracts International, Volume: 85-01.

Thesis (M.S.)--Southeastern Louisiana University, 2023.

This item must not be sold to any third party vendors.

The African Turquoise Killifish (N. furzeri) is a newly emerging model organism in the study of aging and age-related diseases. The incredibly short lifespan of N. furzeri (10-12 weeks) in captivity and the phenotypic hallmarks of aging make it an ideal system for conducting experiments. In this study, I examined the gene expression profiles of killifishes (Cyprinodontiformes: Nothobranchiidae) with different life-histories (annuals, non-annuals, and semi-annuals) using liver and brain tissues. Annual killifish produce diapause eggs and complete their life cycle within a year due to the annual desiccation of the habitat. Non-annual killifish do not lay diapause eggs and live multiple years in permanent water bodies. Semi-annual or facultative-annual killifish can produce eggs that survive the dry season and live for more than a year if conditions are appropriate. My results show that in liver tissue, the gene expression profiles of nothobranchids clustered by their phylogenetic relationships. Annual species show upregulation in DNA repair and chromatin remodeling genes compared to non-annual and semi-annual species, which is inconsistent with previous findings in the literature. Most of the enriched Gene Ontology terms in annual species are related to metabolic processes. However, GO terms including translation, protein transport, and DNA replication initiation are also enriched in annual species, suggesting an accelerated lifespan. Non-annual species are enriched in Notch signaling pathway and downregulated in the canonical Wnt signaling pathway compared to annual species, which might suggest non-annual species have better regulation in cellular processes including cell proliferation, cell death, and homeostasis in the early adult stage. In brain tissue, the gene expression profiles of nothobranchids are clustered by life-histories. I examined six novel genes related to neurogenesis in which only DNMT3A was found to be differentially expressed in annual species. Similar with the liver tissue, the Notch signaling pathway, which was previously identified to be age-dependent and involved in neurogenesis in N. furzeri, is upregulated in non-annual species compared to annual species. Annual species are also enriched in ribosome and oxidative phosphorylation pathways compared to other life-histories. This is consistent with the previous findings in the literature where the upregulation of ribosome and oxidative phosphorylation were found to be inversely correlated with the lifespan of N. furzeri. 

ISBN: 9798379896683Subjects--Topical Terms:

522710
Biology.
Subjects--Index Terms:

African Turquoise Killifish

Examining the Gene Expression Profiles of Killifishes With Different Life-Histories Using Liver and Brain Tissues (Cyprinodontiformes: Nothobranchiidae).
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245 1 0 $a Examining the Gene Expression Profiles of Killifishes With Different Life-Histories Using Liver and Brain Tissues (Cyprinodontiformes: Nothobranchiidae).
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 101 p.
500 $a Source: Masters Abstracts International, Volume: 85-01.
500 $a Advisor: Piller, Kyle R.
502 $a Thesis (M.S.)--Southeastern Louisiana University, 2023.
506 $a This item must not be sold to any third party vendors.
520 $a The African Turquoise Killifish (N. furzeri) is a newly emerging model organism in the study of aging and age-related diseases. The incredibly short lifespan of N. furzeri (10-12 weeks) in captivity and the phenotypic hallmarks of aging make it an ideal system for conducting experiments. In this study, I examined the gene expression profiles of killifishes (Cyprinodontiformes: Nothobranchiidae) with different life-histories (annuals, non-annuals, and semi-annuals) using liver and brain tissues. Annual killifish produce diapause eggs and complete their life cycle within a year due to the annual desiccation of the habitat. Non-annual killifish do not lay diapause eggs and live multiple years in permanent water bodies. Semi-annual or facultative-annual killifish can produce eggs that survive the dry season and live for more than a year if conditions are appropriate. My results show that in liver tissue, the gene expression profiles of nothobranchids clustered by their phylogenetic relationships. Annual species show upregulation in DNA repair and chromatin remodeling genes compared to non-annual and semi-annual species, which is inconsistent with previous findings in the literature. Most of the enriched Gene Ontology terms in annual species are related to metabolic processes. However, GO terms including translation, protein transport, and DNA replication initiation are also enriched in annual species, suggesting an accelerated lifespan. Non-annual species are enriched in Notch signaling pathway and downregulated in the canonical Wnt signaling pathway compared to annual species, which might suggest non-annual species have better regulation in cellular processes including cell proliferation, cell death, and homeostasis in the early adult stage. In brain tissue, the gene expression profiles of nothobranchids are clustered by life-histories. I examined six novel genes related to neurogenesis in which only DNMT3A was found to be differentially expressed in annual species. Similar with the liver tissue, the Notch signaling pathway, which was previously identified to be age-dependent and involved in neurogenesis in N. furzeri, is upregulated in non-annual species compared to annual species. Annual species are also enriched in ribosome and oxidative phosphorylation pathways compared to other life-histories. This is consistent with the previous findings in the literature where the upregulation of ribosome and oxidative phosphorylation were found to be inversely correlated with the lifespan of N. furzeri. 
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650 4 $a Limnology. $3 545788
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650 4 $a Genetics. $3 530508
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653 $a DNA repair
653 $a Life-history
653 $a Longevity
653 $a Neurogenesis
653 $a Nothobranchius furzeri
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