東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Toward Expanding the Utility of Ferm...

Ingle, Abel Trinidad.

FindBook

Google Book

Amazon

博客來

Toward Expanding the Utility of Fermentative Microbiomes for the Recovery of Chemical Energy in Agroindustrial Residues.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Toward Expanding the Utility of Fermentative Microbiomes for the Recovery of Chemical Energy in Agroindustrial Residues./
作者:	Ingle, Abel Trinidad.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	165 p.
附註:	Source: Dissertations Abstracts International, Volume: 84-12, Section: A.
Contained By:	Dissertations Abstracts International84-12A.
標題:	Environmental engineering. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30529891
ISBN:	9798379733704

Toward Expanding the Utility of Fermentative Microbiomes for the Recovery of Chemical Energy in Agroindustrial Residues.
Ingle, Abel Trinidad.

Toward Expanding the Utility of Fermentative Microbiomes for the Recovery of Chemical Energy in Agroindustrial Residues. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 165 p.

Source: Dissertations Abstracts International, Volume: 84-12, Section: A.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2023.

This item must not be sold to any third party vendors.

Agroindustrial operations inherently generate residues, which possess chemical energy. Recovering this chemical energy as valuable chemicals, such as biofuel or bioproduct precursors, can provide sustainability to the agroindustry. Many agricultural residues are lignocellulosic materials that contain carbohydrates stored in the form of cellulose and hemicellulose. Depolymerization of these carbohydrate polymers produces carbohydrate-rich hydrolysates that can be fed to microorganisms to generate fermentation products of value to society. When the organic material in lignocellulosic hydrolysates is complex in composition, fermentative microbiomes have been proposed as a biotechnological means to ferment the carbohydrates in the hydrolysates and recover valuable fermentation products. However, harnessing microbiomes for production of valuable fermentation products remains a challenging task because the genomic potential of the microbial communities that self-assemble in bioreactors fed hydrolysates is difficult to predict.In this work, we evaluated the possibility of depolymerizing the lignocellulosic fibers found in dairy manure to create carbohydrate-rich hydrolysates amenable to fermentation. We demonstrated that a thermochemical method could be used to create glucose and xylose-rich hydrolysates, and that these hydrolysates could be fermented to a variety of fermentation products including acetic acid, lactic acid, succinic acid, butyric acid, hexanoic acid, and octanoic acid (Chapter 2). To gain insight into the genomic potential of the microorganisms enriched in the fermentation process we performed metagenomic analyses and recovered metagenome assembled genomes (MAGs) from abundant members of the community (Chapter 3). To evaluate the genomic potential of the assembled genomes we developed a procedure to query the metagenomes for the presence of genes encoding homologues of key enzymes in the fermentation process. Metabolic maps of key fermentation processes were assembled, and a combination of gene annotations and sequence comparison was used to determine whether the presence of key enzymes could be established in the MAGs (Chapter 3). This analysis revealed the capability of lactic acid production on the majority of MAGs and the capability of chain-elongation on a smaller number of MAGs. Finally, based on the observations of which organisms were capable of chain-elongation, and the assessment of their genomic potential for substrate utilization and for maintaining redox balance, we hypothesized that the ratio of reduced ferredoxin to NADH plays an important role in determining the end product of the cyclic chain-elongation process. In summary, this project provides an alternative approach to potentially gain value from manure by fermenting carbohydrate-rich hydrolysates produced by thermochemical processes, establishes the capacity of microbiomes to ferment these hydrolysates into a variety of fermentation products, and provides approaches to analyze the genomic potential of the microorganisms in fermenting microbiomes, an important task that needs further refinement to be able to ultimately harness the power of microbiomes for generating value from agroindustrial residues.

ISBN: 9798379733704Subjects--Topical Terms:

548583
Environmental engineering.
Subjects--Index Terms:

Agroindustrial residue

Toward Expanding the Utility of Fermentative Microbiomes for the Recovery of Chemical Energy in Agroindustrial Residues.
LDR:04548nmm a2200409 4500 001 2393069
005 20231130111631.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798379733704
035 $a (MiAaPQ)AAI30529891
035 $a AAI30529891
040 $a MiAaPQ $c MiAaPQ
100 1 $a Ingle, Abel Trinidad. $3 3762510
245 1 0 $a Toward Expanding the Utility of Fermentative Microbiomes for the Recovery of Chemical Energy in Agroindustrial Residues.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 165 p.
500 $a Source: Dissertations Abstracts International, Volume: 84-12, Section: A.
500 $a Advisor: Noguera, Daniel R.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2023.
506 $a This item must not be sold to any third party vendors.
520 $a Agroindustrial operations inherently generate residues, which possess chemical energy. Recovering this chemical energy as valuable chemicals, such as biofuel or bioproduct precursors, can provide sustainability to the agroindustry. Many agricultural residues are lignocellulosic materials that contain carbohydrates stored in the form of cellulose and hemicellulose. Depolymerization of these carbohydrate polymers produces carbohydrate-rich hydrolysates that can be fed to microorganisms to generate fermentation products of value to society. When the organic material in lignocellulosic hydrolysates is complex in composition, fermentative microbiomes have been proposed as a biotechnological means to ferment the carbohydrates in the hydrolysates and recover valuable fermentation products. However, harnessing microbiomes for production of valuable fermentation products remains a challenging task because the genomic potential of the microbial communities that self-assemble in bioreactors fed hydrolysates is difficult to predict.In this work, we evaluated the possibility of depolymerizing the lignocellulosic fibers found in dairy manure to create carbohydrate-rich hydrolysates amenable to fermentation. We demonstrated that a thermochemical method could be used to create glucose and xylose-rich hydrolysates, and that these hydrolysates could be fermented to a variety of fermentation products including acetic acid, lactic acid, succinic acid, butyric acid, hexanoic acid, and octanoic acid (Chapter 2). To gain insight into the genomic potential of the microorganisms enriched in the fermentation process we performed metagenomic analyses and recovered metagenome assembled genomes (MAGs) from abundant members of the community (Chapter 3). To evaluate the genomic potential of the assembled genomes we developed a procedure to query the metagenomes for the presence of genes encoding homologues of key enzymes in the fermentation process. Metabolic maps of key fermentation processes were assembled, and a combination of gene annotations and sequence comparison was used to determine whether the presence of key enzymes could be established in the MAGs (Chapter 3). This analysis revealed the capability of lactic acid production on the majority of MAGs and the capability of chain-elongation on a smaller number of MAGs. Finally, based on the observations of which organisms were capable of chain-elongation, and the assessment of their genomic potential for substrate utilization and for maintaining redox balance, we hypothesized that the ratio of reduced ferredoxin to NADH plays an important role in determining the end product of the cyclic chain-elongation process. In summary, this project provides an alternative approach to potentially gain value from manure by fermenting carbohydrate-rich hydrolysates produced by thermochemical processes, establishes the capacity of microbiomes to ferment these hydrolysates into a variety of fermentation products, and provides approaches to analyze the genomic potential of the microorganisms in fermenting microbiomes, an important task that needs further refinement to be able to ultimately harness the power of microbiomes for generating value from agroindustrial residues.
590 $a School code: 0262.
650 4 $a Environmental engineering. $3 548583
650 4 $a Microbiology. $3 536250
650 4 $a Alternative energy. $3 3436775
650 4 $a Sustainability. $3 1029978
653 $a Agroindustrial residue
653 $a Chain-elongation
653 $a Dairy manure
653 $a Fermentation
653 $a Medium-chain fatty acid
690 $a 0775
690 $a 0410
690 $a 0640
690 $a 0363
710 2 $a The University of Wisconsin - Madison. $b Civil & Environmental Engr. $3 2097812
773 0 $t Dissertations Abstracts International $g 84-12A.
790 $a 0262
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30529891