東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Regulators of Mast Cell Activation.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Regulators of Mast Cell Activation./
作者:	Lyons, David.
面頁冊數:	1 online resource (157 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-11, Section: B.
Contained By:	Dissertations Abstracts International84-11B.
標題:	Immunology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30490938click for full text (PQDT)
ISBN:	9798379516956

Regulators of Mast Cell Activation.
Lyons, David.

Regulators of Mast Cell Activation. - 1 online resource (157 pages)

Source: Dissertations Abstracts International, Volume: 84-11, Section: B.

Thesis (Ph.D.)--University of Northern Colorado, 2023.

Includes bibliographical references

This research focuses on identifying novel mechanisms through which mast cells (MCs) can be activated, specifically focusing on the effects of transforming growth factor beta-1 (TGFβ1) on MC activation and cytokine release and how these pathways can contribute to disease. We found that MCs can be activated with TGF-β1 alone, inducing degranulation and cytokine release comparable to classical, immunoglobulin-E-mediated activation paradigms. This activated state resembles a described concept in immunology termed trained immunity - a process that allows innate immune cells to potentiate their future immune responses following priming with a stimulus. Hypothesizing that TGF-β1 is a novel stimulus of trained immunity, we observed the metabolic and gene expression changes in MCs stimulated with TGF-β1 in comparison to lipopolysaccharide stimulation to better understand the biological changes associated with a trained immune phenotype. We discovered that priming of MCs induced significant changes in inflammatory and inflammasome-related gene expression during initial priming and restimulation almost a week later, however there were no observable metabolic changes. These findings indicate that MCs respond to secreted cytokines, coupled with gene expression changes, and have the biological machinery associated with inflammasome activation and trained immunity. However, they do not alter their metabolic phenotype to these cues. Taken together, these discoveries illustrate a new role for the MC in various diseases that present with chronic inflammation including cancers. This research includes a review of literature documenting the involvement of MCs in the propagation and persistence of inflammatory diseases. To further study this, MCs were identified in murine mammary adenocarcinomas and found to be significantly increased in tumors lacking the interleukin-6 gene. Given MCs were originally identified within a breast cancer tumor, this discovery suggests that MCs are a critical inflammatory cell whose function can be aberrantly altered in specific tissues, contributing to chronic inflammatory diseases. Mast cells are expressed in almost all tissue types - this study aims to highlight the significance of MCs in inflammation and suggests novel therapeutic options which aim to reduce remove the MC from the inflammatory triad of tissue damage, cytokine release, and immune cell migration/invasion into tissue. Future directions will continue to describe this newly appreciated role for the MC and identify the long-term effects of trained immunity on MC function and activation.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379516956Subjects--Topical Terms:

611031
Immunology.
Subjects--Index Terms:

CytokineIndex Terms--Genre/Form:

542853
Electronic books.

Regulators of Mast Cell Activation.
LDR:03973nmm a2200409K 4500 001 2363965
005 20231127094747.5
006 m o d
007 cr mn ---uuuuu
008 241011s2023 xx obm 000 0 eng d
020 $a 9798379516956
035 $a (MiAaPQ)AAI30490938
035 $a AAI30490938
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Lyons, David. $3 3704744
245 1 0 $a Regulators of Mast Cell Activation.
264 0 $c 2023
300 $a 1 online resource (157 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 84-11, Section: B.
500 $a Advisor: Pullen, Nicholas A.
502 $a Thesis (Ph.D.)--University of Northern Colorado, 2023.
504 $a Includes bibliographical references
520 $a This research focuses on identifying novel mechanisms through which mast cells (MCs) can be activated, specifically focusing on the effects of transforming growth factor beta-1 (TGFβ1) on MC activation and cytokine release and how these pathways can contribute to disease. We found that MCs can be activated with TGF-β1 alone, inducing degranulation and cytokine release comparable to classical, immunoglobulin-E-mediated activation paradigms. This activated state resembles a described concept in immunology termed trained immunity - a process that allows innate immune cells to potentiate their future immune responses following priming with a stimulus. Hypothesizing that TGF-β1 is a novel stimulus of trained immunity, we observed the metabolic and gene expression changes in MCs stimulated with TGF-β1 in comparison to lipopolysaccharide stimulation to better understand the biological changes associated with a trained immune phenotype. We discovered that priming of MCs induced significant changes in inflammatory and inflammasome-related gene expression during initial priming and restimulation almost a week later, however there were no observable metabolic changes. These findings indicate that MCs respond to secreted cytokines, coupled with gene expression changes, and have the biological machinery associated with inflammasome activation and trained immunity. However, they do not alter their metabolic phenotype to these cues. Taken together, these discoveries illustrate a new role for the MC in various diseases that present with chronic inflammation including cancers. This research includes a review of literature documenting the involvement of MCs in the propagation and persistence of inflammatory diseases. To further study this, MCs were identified in murine mammary adenocarcinomas and found to be significantly increased in tumors lacking the interleukin-6 gene. Given MCs were originally identified within a breast cancer tumor, this discovery suggests that MCs are a critical inflammatory cell whose function can be aberrantly altered in specific tissues, contributing to chronic inflammatory diseases. Mast cells are expressed in almost all tissue types - this study aims to highlight the significance of MCs in inflammation and suggests novel therapeutic options which aim to reduce remove the MC from the inflammatory triad of tissue damage, cytokine release, and immune cell migration/invasion into tissue. Future directions will continue to describe this newly appreciated role for the MC and identify the long-term effects of trained immunity on MC function and activation.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Immunology. $3 611031
650 4 $a Biology. $3 522710
650 4 $a Cellular biology. $3 3172791
650 4 $a Molecular biology. $3 517296
653 $a Cytokine
653 $a Flow cytometry
653 $a Interleukin
653 $a Mast cell
653 $a Transforming growth factor beta-1
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0982
690 $a 0306
690 $a 0379
690 $a 0307
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a University of Northern Colorado. $b Biological Science. $3 2102744
773 0 $t Dissertations Abstracts International $g 84-11B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30490938 $z click for full text (PQDT)