東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Linked to FindBook

Google Book

Amazon

博客來

Characterization of exopolysaccharide biosynthetic genes - wceF, wceJ, wzx1, of Pantoea stewartii subsp. stewartii and their corresponding homologues of Erwinia amylovora.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of exopolysaccharide biosynthetic genes - wceF, wceJ, wzx1, of Pantoea stewartii subsp. stewartii and their corresponding homologues of Erwinia amylovora./
Author:	Wang, Xiaolei.
Description:	1 online resource (95 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 73-08, Section: B.
Contained By:	Dissertations Abstracts International73-08B.
Subject:	Plant biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3492125click for full text (PQDT)
ISBN:	9781267112507

Characterization of exopolysaccharide biosynthetic genes - wceF, wceJ, wzx1, of Pantoea stewartii subsp. stewartii and their corresponding homologues of Erwinia amylovora.
Wang, Xiaolei.

Characterization of exopolysaccharide biosynthetic genes - wceF, wceJ, wzx1, of Pantoea stewartii subsp. stewartii and their corresponding homologues of Erwinia amylovora. - 1 online resource (95 pages)

Source: Dissertations Abstracts International, Volume: 73-08, Section: B.

Thesis (Ph.D.)--University of Connecticut, 2011.

Includes bibliographical references

Pantoea stewartii subsp. stewartii and Erwinia amylovora are both Gram-negative, xylem dwelling phytopathogens. Cell density-dependent production of capsular/exopolysaccharide (CPS/EPS) is required for the successful colonization within the host's xylem and disease development for both pathogens. The gene clusters encoding the machinery responsible for the biosynthesis of their EPS have been widely studied but the annotations of a few members of the gene clusters heavily rely on bioinformatics predication without any experimental evidence. In this dissertation research, I used genetic and biochemical approaches to validate the function of asmJ and asmL of E. amylovora. They serve as pyruvyl transferase and amylovoran repeating unit flippase, respectively just as predicted. I also showed their corresponding homologues-wceJ and wzx1 does not contribute to the biosynthesis of native stewartan EPS. WceJ is non-functional due to mutation(s) of key amino acid residue(s) while Wzx1 is exclusively responsible for translocating non-native, pyruvylated stewartan repeating units. I also revealed the presence of a high-molecular-weight (HMW), lipid A-linked stewartan and this polymer is subject to WceF-mediated degradation. The degradation of cell-bound HMW stewartan offers a significant level of protection against stewartan-dependent bacteriophage by minimizing the number of surface receptors.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9781267112507Subjects--Topical Terms:

3186449
Plant biology.
Subjects--Index Terms:

Erwinia amylovoraIndex Terms--Genre/Form:

542853
Electronic books.

Characterization of exopolysaccharide biosynthetic genes - wceF, wceJ, wzx1, of Pantoea stewartii subsp. stewartii and their corresponding homologues of Erwinia amylovora.
LDR:02982nmm a2200421K 4500 001 2360314
005 20230926101838.5
006 m o d
007 cr mn ---uuuuu
008 241011s2011 xx obm 000 0 eng d
020 $a 9781267112507
035 $a (MiAaPQ)AAI3492125
035 $a AAI3492125
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Wang, Xiaolei. $3 2132555
245 1 0 $a Characterization of exopolysaccharide biosynthetic genes - wceF, wceJ, wzx1, of Pantoea stewartii subsp. stewartii and their corresponding homologues of Erwinia amylovora.
264 0 $c 2011
300 $a 1 online resource (95 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 73-08, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Bodman, von, Susanne.
502 $a Thesis (Ph.D.)--University of Connecticut, 2011.
504 $a Includes bibliographical references
520 $a Pantoea stewartii subsp. stewartii and Erwinia amylovora are both Gram-negative, xylem dwelling phytopathogens. Cell density-dependent production of capsular/exopolysaccharide (CPS/EPS) is required for the successful colonization within the host's xylem and disease development for both pathogens. The gene clusters encoding the machinery responsible for the biosynthesis of their EPS have been widely studied but the annotations of a few members of the gene clusters heavily rely on bioinformatics predication without any experimental evidence. In this dissertation research, I used genetic and biochemical approaches to validate the function of asmJ and asmL of E. amylovora. They serve as pyruvyl transferase and amylovoran repeating unit flippase, respectively just as predicted. I also showed their corresponding homologues-wceJ and wzx1 does not contribute to the biosynthesis of native stewartan EPS. WceJ is non-functional due to mutation(s) of key amino acid residue(s) while Wzx1 is exclusively responsible for translocating non-native, pyruvylated stewartan repeating units. I also revealed the presence of a high-molecular-weight (HMW), lipid A-linked stewartan and this polymer is subject to WceF-mediated degradation. The degradation of cell-bound HMW stewartan offers a significant level of protection against stewartan-dependent bacteriophage by minimizing the number of surface receptors.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Plant biology. $3 3186449
650 4 $a Genetics. $3 530508
650 4 $a Microbiology. $3 536250
650 4 $a Botany. $3 516217
653 $a Erwinia amylovora
653 $a Exopolysaccharide
653 $a Pantoea stewartii
653 $a Plant disease
653 $a Pyruvyl transferase
653 $a Terminal glucosyl transferase
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0309
690 $a 0369
690 $a 0410
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a University of Connecticut. $3 1017435
773 0 $t Dissertations Abstracts International $g 73-08B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3492125 $z click for full text (PQDT)