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Ultrasound Stimulation of Insulin Release from Pancreatic Beta Cells.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Ultrasound Stimulation of Insulin Release from Pancreatic Beta Cells./
Author:	Suarez Castellanos, Ivan M.
Description:	1 online resource (164 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 79-02, Section: B.
Contained By:	Dissertations Abstracts International79-02B.
Subject:	Cellular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10599802click for full text (PQDT)
ISBN:	9780355070002

Ultrasound Stimulation of Insulin Release from Pancreatic Beta Cells.
Suarez Castellanos, Ivan M.

Ultrasound Stimulation of Insulin Release from Pancreatic Beta Cells. - 1 online resource (164 pages)

Source: Dissertations Abstracts International, Volume: 79-02, Section: B.

Thesis (Ph.D.)--The George Washington University, 2017.

Includes bibliographical references

Type 2 diabetes (T2D) mellitus is a complex metabolic disease that has reached epidemic proportions in the United States and around the world. Controlling T2D is often difficult as pharmacological management routinely requires complex therapy with multiple medications, and loses its effectiveness over time. The objective of this dissertation was to explore a novel, non-pharmacological approach that utilizes the application of ultrasound energy to stimulate insulin release. Our experiments have focused on determination of effectiveness and safety of ultrasound application in stimulation of insulin release from the pancreatic beta cells. Our results showed that ultrasound treatment, applied at frequencies of 800 kHz and 1 MHz and intensities of 0.5 W/cm2 and 1 W/cm2, did not produce any significant effects on cell viability compared to sham group as assessed with trypan blue dye exclusion test and MTT cytotoxicity assay. ELISA quantification of insulin release from beta cells resulting from ultrasound treatment showed clinically-significant amounts of released insulin as compared to sham-treated beta cells. Carbon fiber amperometry detection of secretory events from dopamine-loaded beta cells treated with ultrasound showed that release of secretory content could be temporally controlled by careful selection of ultrasound parameters. Both ELISA and amperometry experiments demonstrated that ultrasound-stimulated insulin release is a calcium-dependent process, potentially mediated by the mechanical effects of ultrasound. This study demonstrated that therapeutic ultrasound is a technique capable of stimulating the release of insulin from pancreatic beta cells in a safe, effective and controlled manner.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9780355070002Subjects--Topical Terms:

3172791
Cellular biology.
Subjects--Index Terms:

Beta cellsIndex Terms--Genre/Form:

542853
Electronic books.

Ultrasound Stimulation of Insulin Release from Pancreatic Beta Cells.
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502 $a Thesis (Ph.D.)--The George Washington University, 2017.
504 $a Includes bibliographical references
520 $a Type 2 diabetes (T2D) mellitus is a complex metabolic disease that has reached epidemic proportions in the United States and around the world. Controlling T2D is often difficult as pharmacological management routinely requires complex therapy with multiple medications, and loses its effectiveness over time. The objective of this dissertation was to explore a novel, non-pharmacological approach that utilizes the application of ultrasound energy to stimulate insulin release. Our experiments have focused on determination of effectiveness and safety of ultrasound application in stimulation of insulin release from the pancreatic beta cells. Our results showed that ultrasound treatment, applied at frequencies of 800 kHz and 1 MHz and intensities of 0.5 W/cm2 and 1 W/cm2, did not produce any significant effects on cell viability compared to sham group as assessed with trypan blue dye exclusion test and MTT cytotoxicity assay. ELISA quantification of insulin release from beta cells resulting from ultrasound treatment showed clinically-significant amounts of released insulin as compared to sham-treated beta cells. Carbon fiber amperometry detection of secretory events from dopamine-loaded beta cells treated with ultrasound showed that release of secretory content could be temporally controlled by careful selection of ultrasound parameters. Both ELISA and amperometry experiments demonstrated that ultrasound-stimulated insulin release is a calcium-dependent process, potentially mediated by the mechanical effects of ultrasound. This study demonstrated that therapeutic ultrasound is a technique capable of stimulating the release of insulin from pancreatic beta cells in a safe, effective and controlled manner.
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538 $a Mode of access: World Wide Web
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650 4 $a Acoustics. $3 879105
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653 $a Calcium
653 $a Cell stimulation
653 $a Insulin secretion
653 $a Therapeutic ultrasound
653 $a Type 2 diabetes
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