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Cytogenetics and cell biology of nucleolar tau.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Cytogenetics and cell biology of nucleolar tau./
Author:	Thurston, Virginia Carol.
Description:	1 online resource (126 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 59-04, Section: B.
Contained By:	Dissertations Abstracts International59-04B.
Subject:	Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9734456click for full text (PQDT)
ISBN:	9780591440713

Cytogenetics and cell biology of nucleolar tau.
Thurston, Virginia Carol.

Cytogenetics and cell biology of nucleolar tau. - 1 online resource (126 pages)

Source: Dissertations Abstracts International, Volume: 59-04, Section: B.

Thesis (Ph.D.)--The University of Alabama at Birmingham, 1997.

Includes bibliographical references

Tau is a neuronal microtubule-associated protein (MAP) which localizes primarily in the axons and somatodendritic compartment of neurons (Binder et al., 1985; Papasozomenos and Binder, 1987). In this study we have described our discoveries ofthe localization of tau outside the central nervous system (CNS), upregulation of tau in actively dividing cells, and confirmation of tau in the nucleolus. Tau proteins appear to have functions in addition to cross-linking and stabilizing microtubules within the axon. Nuclear tau was localized via the Tau-1 monoclonal antibody to nucleoli and nucleolar organizer regions (NORs) of acrocentric chromosomes within HeLa cells and in primary cultures of human fibroblasts, lymphocytes, and lymphoblasts. We also present evidence indicating that Tau-1 staining may be a heritable characteristic reminiscent of silver staining, and that Tau-1 immunostaining is upregulated in dividing lymphocytes. Transfection experiments involving tau sense plasmids demonstrated an increase in nucleolar tau in a subset of cells as compared to endogenous tau by quantitative image analysis. Transient transfection of tau anti-sense plasmids abolished nucleolar Tau-1 staining. This further substantiated the presence of tau in the nucleolus of neuronal cell lines as well as nonneuronal human cells, and strongly suggested that Tau-1 nucleolar staining was due to the presence of tau proteins and not some cross-reacting species. These studies have contributed to the growing evidence that tau was a multifunctional protein found not only associated with microtubles in the axons and somatodendritic compartments of neurons, but also localized to the nucleolus and NORs in neuroblastomas, HeLa cells, human skin fibroblasts and lymphocytes.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9780591440713Subjects--Topical Terms:

530508
Genetics.
Subjects--Index Terms:

microtubule-associatedIndex Terms--Genre/Form:

542853
Electronic books.

Cytogenetics and cell biology of nucleolar tau.
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504 $a Includes bibliographical references
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