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Characterization of microtubule assembly of beta-tubulin isotypes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Characterization of microtubule assembly of beta-tubulin isotypes./
作者:	Lu, Qing.
面頁冊數:	1 online resource (115 pages)
附註:	Source: Dissertations Abstracts International, Volume: 56-06, Section: B.
Contained By:	Dissertations Abstracts International56-06B.
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9500860click for full text (PQDT)
ISBN:	9798209406433

Characterization of microtubule assembly of beta-tubulin isotypes.
Lu, Qing.

Characterization of microtubule assembly of beta-tubulin isotypes. - 1 online resource (115 pages)

Source: Dissertations Abstracts International, Volume: 56-06, Section: B.

Thesis (Ph.D.)--The University of Texas Health Science Center at San Antonio, 1994.

Includes bibliographical references

Microtubule assembly of different beta-tubulin isotypes in the presence of 4 M glycerol and 6 mM magnesium ion demonstrates significantly different characteristics. Beta II and beta IV assembled faster and to a greater extent than did phosphocellulose purified tubulin (PC-tubulin). Microtubule assembly from beta III showed a distinctive delay in nucleation. However, treatment of beta-tubulin isotypes with subtilisin to remove the C-terminal domain of the tubulin dimer abolished these differences in microtubule assembly pattern. Elongation of beta III from microtubule seeds had a lower association constant and a lower dissociation constant than did beta II and beta IV. The initial rates of GTPase activities of beta II and beta IV were the highest, while that of beta III was the lowest during the nucleation phase of microtubule assembly. During microtubule elongation, the rate of GTP hydrolysis was the lowest for beta III. The GTPase activity of colchicine complexes of beta III was significantly higher than that of other beta-tubulin isotypes. Microtubule assembly of PC-tubulin, beta II, beta III and beta IV in the presence of Chromium GTP proceeded at a slower rate and to a lesser extent than that in the presence of Magnesium GTP. After cold-induced depolymerization, beta III lost its ability to reassemble into microtubles while PC-tubulin, beta II and beta IV could reassemble into microtubules. The study of the kinetics of the hydrolysis of CrGTP showed that beta III isotype was less efficient in hydrolyzing Chromium GTP. Our studies have shown that the isotypic composition of beta-tubulin exerts a great effects on the kinetics and dynamic behavior of microtubule assembly. The GTPase activity of tubulin during microtubule nucleation and elongation also varied among the beta-tubulin isotypes. The present study provides some further insight about the functional effects of different beta-tubulin isotypes on microtubule assembly and GTPase activity. It enables us to further investigate the functional role of different tubulin isotypes in vivo and in vitro.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798209406433Subjects--Topical Terms:

3172791
Cellular biology.
Subjects--Index Terms:

tubulinIndex Terms--Genre/Form:

542853
Electronic books.

Characterization of microtubule assembly of beta-tubulin isotypes.
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504 $a Includes bibliographical references
520 $a Microtubule assembly of different beta-tubulin isotypes in the presence of 4 M glycerol and 6 mM magnesium ion demonstrates significantly different characteristics. Beta II and beta IV assembled faster and to a greater extent than did phosphocellulose purified tubulin (PC-tubulin). Microtubule assembly from beta III showed a distinctive delay in nucleation. However, treatment of beta-tubulin isotypes with subtilisin to remove the C-terminal domain of the tubulin dimer abolished these differences in microtubule assembly pattern. Elongation of beta III from microtubule seeds had a lower association constant and a lower dissociation constant than did beta II and beta IV. The initial rates of GTPase activities of beta II and beta IV were the highest, while that of beta III was the lowest during the nucleation phase of microtubule assembly. During microtubule elongation, the rate of GTP hydrolysis was the lowest for beta III. The GTPase activity of colchicine complexes of beta III was significantly higher than that of other beta-tubulin isotypes. Microtubule assembly of PC-tubulin, beta II, beta III and beta IV in the presence of Chromium GTP proceeded at a slower rate and to a lesser extent than that in the presence of Magnesium GTP. After cold-induced depolymerization, beta III lost its ability to reassemble into microtubles while PC-tubulin, beta II and beta IV could reassemble into microtubules. The study of the kinetics of the hydrolysis of CrGTP showed that beta III isotype was less efficient in hydrolyzing Chromium GTP. Our studies have shown that the isotypic composition of beta-tubulin exerts a great effects on the kinetics and dynamic behavior of microtubule assembly. The GTPase activity of tubulin during microtubule nucleation and elongation also varied among the beta-tubulin isotypes. The present study provides some further insight about the functional effects of different beta-tubulin isotypes on microtubule assembly and GTPase activity. It enables us to further investigate the functional role of different tubulin isotypes in vivo and in vitro.
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538 $a Mode of access: World Wide Web
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