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The Design, Construction, And Testing of a Recombinant DNA Vaccine for Brucella Abortus and Brucella Melitensis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Design, Construction, And Testing of a Recombinant DNA Vaccine for Brucella Abortus and Brucella Melitensis./
Author:	Korle, Stephanie Lynn.
Description:	1 online resource (155 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 84-02, Section: B.
Contained By:	Dissertations Abstracts International84-02B.
Subject:	Infections. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29221738click for full text (PQDT)
ISBN:	9798841531173

The Design, Construction, And Testing of a Recombinant DNA Vaccine for Brucella Abortus and Brucella Melitensis.
Korle, Stephanie Lynn.

The Design, Construction, And Testing of a Recombinant DNA Vaccine for Brucella Abortus and Brucella Melitensis. - 1 online resource (155 pages)

Source: Dissertations Abstracts International, Volume: 84-02, Section: B.

Thesis (Ph.D.)--Louisiana State University and Agricultural & Mechanical College, 2022.

Includes bibliographical references

Brucella spp. are a bacterium that cause brucellosis, a zoonotic disease, which is commonly seen in cattle, sheep, goats, swine, and canines. Brucellosis is a problem worldwide, although it is eradicated in some countries (Garin-Bastuji et al. 1998). The reason for designing recombinant DNA (rDNA) vaccines opposed to utilizing the live-attenuated vaccines on the market is that they cannot be given to pregnant animals without potentially causing abortion, while an rDNA vaccine should be safe for pregnant animals since it does not contain viable bacteria. Also, there are no serological tests that can accurately distinguish between an animal vaccinated with the current vaccines available for brucellosis and an animal with an active Brucella infection because the current vaccines are live-attenuated and interfere with diagnostics. Recombinant DNA vaccines eliminate many safety concerns, which is why two rDNA vaccines were constructed in the same way, except for the length of the immunogenic epitopes selected from Brucella abortus.The epitopes from B. abortus that were chosen are present in the other main species of Brucella because the main species of Brucella, such as B. abortus, B. melitensis, and B. suis, are 99% identical at the DNA level.The epitopes range in length from 25 to 70 amino acids. Each vaccine contained a specific insert, with different lengths of epitopes and an antibiotic resistance gene that was incorporated for selection of successfully transfected cells during in vitro testing. After the vaccines were constructed, they were tested in vitro to determine the vaccine's ability to enter different cell types and show protein expression in a variety of cells. The vaccines were tested in a caprine model to determine the vaccine's ability to be successfully expressed in mammalian cells in vivo. It was determined that no significant antibody concentration was detected via the testing methods utilized.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798841531173Subjects--Topical Terms:

1621997
Infections.
Index Terms--Genre/Form:

542853
Electronic books.

The Design, Construction, And Testing of a Recombinant DNA Vaccine for Brucella Abortus and Brucella Melitensis.
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020 $a 9798841531173
035 $a (MiAaPQ)AAI29221738
035 $a (MiAaPQ)Louisianagradschool_dissertations-6866
035 $a AAI29221738
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Korle, Stephanie Lynn. $3 3696589
245 1 4 $a The Design, Construction, And Testing of a Recombinant DNA Vaccine for Brucella Abortus and Brucella Melitensis.
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300 $a 1 online resource (155 pages)
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500 $a Source: Dissertations Abstracts International, Volume: 84-02, Section: B.
500 $a Advisor: Cooper, Richard.
502 $a Thesis (Ph.D.)--Louisiana State University and Agricultural & Mechanical College, 2022.
504 $a Includes bibliographical references
520 $a Brucella spp. are a bacterium that cause brucellosis, a zoonotic disease, which is commonly seen in cattle, sheep, goats, swine, and canines. Brucellosis is a problem worldwide, although it is eradicated in some countries (Garin-Bastuji et al. 1998). The reason for designing recombinant DNA (rDNA) vaccines opposed to utilizing the live-attenuated vaccines on the market is that they cannot be given to pregnant animals without potentially causing abortion, while an rDNA vaccine should be safe for pregnant animals since it does not contain viable bacteria. Also, there are no serological tests that can accurately distinguish between an animal vaccinated with the current vaccines available for brucellosis and an animal with an active Brucella infection because the current vaccines are live-attenuated and interfere with diagnostics. Recombinant DNA vaccines eliminate many safety concerns, which is why two rDNA vaccines were constructed in the same way, except for the length of the immunogenic epitopes selected from Brucella abortus.The epitopes from B. abortus that were chosen are present in the other main species of Brucella because the main species of Brucella, such as B. abortus, B. melitensis, and B. suis, are 99% identical at the DNA level.The epitopes range in length from 25 to 70 amino acids. Each vaccine contained a specific insert, with different lengths of epitopes and an antibiotic resistance gene that was incorporated for selection of successfully transfected cells during in vitro testing. After the vaccines were constructed, they were tested in vitro to determine the vaccine's ability to enter different cell types and show protein expression in a variety of cells. The vaccines were tested in a caprine model to determine the vaccine's ability to be successfully expressed in mammalian cells in vivo. It was determined that no significant antibody concentration was detected via the testing methods utilized.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Infections. $3 1621997
650 4 $a Vaccines. $3 684854
650 4 $a Manufacturing. $3 3389707
650 4 $a Disease. $3 705846
650 4 $a Mutation. $3 837917
650 4 $a Bacteria. $3 550366
650 4 $a Adaptation. $3 3562958
650 4 $a Metabolism. $3 541349
650 4 $a Apoptosis. $3 600650
650 4 $a Cattle. $3 899336
650 4 $a Drug resistance. $3 1602664
650 4 $a Fetuses. $3 3696590
650 4 $a Plasmids. $3 3319070
650 4 $a Autoimmune diseases. $3 872484
650 4 $a Antibiotics. $3 670318
650 4 $a Immune system. $3 689864
650 4 $a Design. $3 518875
650 4 $a Antigens. $3 700737
650 4 $a Pathogenesis. $3 3561735
650 4 $a Abortion. $3 818164
650 4 $a Polypeptides. $3 1085124
650 4 $a Animal sciences. $3 3174829
650 4 $a Cellular biology. $3 3172791
650 4 $a Immunology. $3 611031
650 4 $a Industrial engineering. $3 526216
650 4 $a Medicine. $3 641104
650 4 $a Pharmaceutical sciences. $3 3173021
650 4 $a Pharmacology. $3 634543
655 7 $a Electronic books. $2 lcsh $3 542853
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690 $a 0546
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710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a Louisiana State University and Agricultural & Mechanical College. $3 783779
773 0 $t Dissertations Abstracts International $g 84-02B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29221738 $z click for full text (PQDT)