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Isolation and Characterization of Fluorescent Proteins and Small Molecules from Marine Vertebrates.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Isolation and Characterization of Fluorescent Proteins and Small Molecules from Marine Vertebrates./
作者:	Krivoshik, Sara Rose.
面頁冊數:	1 online resource (132 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-03, Section: B.
Contained By:	Dissertations Abstracts International84-03B.
標題:	Molecular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29325958click for full text (PQDT)
ISBN:	9798841796572

Isolation and Characterization of Fluorescent Proteins and Small Molecules from Marine Vertebrates.
Krivoshik, Sara Rose.

Isolation and Characterization of Fluorescent Proteins and Small Molecules from Marine Vertebrates. - 1 online resource (132 pages)

Source: Dissertations Abstracts International, Volume: 84-03, Section: B.

Thesis (Ph.D.)--City University of New York, 2022.

Includes bibliographical references

Fluorescence is a phenomenon in which a wavelength of light excites a fluorophore and then emits light at a higher, longer wavelength. This occurs throughout the biological world, in both the terrestrial and marine realms. Encompassing a diverse set of mechanisms from small molecules to large protein complexes; fluorescence has been used as a biological tool with a wide range of applications to study molecular and cellular biology for decades. This dissertation looks to identify and characterize novel fluorescent proteins and small molecules to allow for more efficient translation in developing new biotechnologies. Using a combination of RNA-Seq, Mass-Spec, protein expression and purification, novel fluorescent proteins and small molecules can be identified and characterized.There are several well-described fluorescent fatty-acid binding proteins present throughout the Anguilliformes order, common-name eels. Chlopsid FP is one novel fluorescent fatty acid-binding protein, which has been isolated from the Kaupichthys hyoproroides eel. In this study Chlopsid FP is further characterized by looking at its ability to bind its ligand bilirubin, through a point mutation study. By expanding the characterization of Chlopsid FP the significance of a glycine-proline-proline loop in fluorophore formation is confirmed. Furthermore, several specimens of eels are identified as being fluorescent, including a moray eel, Gymnothorax zonipectis. In doing so fluorescent proteins are implicated as the cause of fluorescence in several new eel specimens, expanding on the current understanding of fluorescent FABPs.In addition, Synodontidae, commonly called Lizardfish, a family of fish displaying both red and green fluorescence, are examined further. Until recently Lizardfish have been overlooked as potential keys to mechanistically understanding fluorescence. Using RNA-Seq technology and analyses, including transcriptomic annotation and differential expression, the Lizardfish family of fish is examined. Specifically, Saurida gracilis and Synodus lucioceps, and their associated fluorescent moieties are studied. The Saurida gracilis is examined due its dual fluorescence, as it expresses both red and green fluorescence. The Synodus lucioceps was selected as a vividly green fluorescent sample. Through examining both species a greater understanding of the Synodontidae family of fish is gained. Furthermore, using different approaches to examine each fish has allowed for the development of an efficient pathway to isolate and identify novel fluorescent moieties from marine organisms. Finally, two shark species, Cephaloscyllium ventriosum and Scyliorhinus retifer, are examined and characterized as cartilaginous fish expressing green fluorescence. A series of novel fluorescent metabolites are identified as small molecules responsible for fluorescence in both species. Additionally, the fluorescent properties of these metabolites, including excitation and emission spectra, are characterized. Ultimately, leading to the identification of a bromo-kynurenine metabolic pathway, furthering the current understanding of fluorescent small molecules.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798841796572Subjects--Topical Terms:

517296
Molecular biology.
Subjects--Index Terms:

FluorescenceIndex Terms--Genre/Form:

542853
Electronic books.

Isolation and Characterization of Fluorescent Proteins and Small Molecules from Marine Vertebrates.
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504 $a Includes bibliographical references
520 $a Fluorescence is a phenomenon in which a wavelength of light excites a fluorophore and then emits light at a higher, longer wavelength. This occurs throughout the biological world, in both the terrestrial and marine realms. Encompassing a diverse set of mechanisms from small molecules to large protein complexes; fluorescence has been used as a biological tool with a wide range of applications to study molecular and cellular biology for decades. This dissertation looks to identify and characterize novel fluorescent proteins and small molecules to allow for more efficient translation in developing new biotechnologies. Using a combination of RNA-Seq, Mass-Spec, protein expression and purification, novel fluorescent proteins and small molecules can be identified and characterized.There are several well-described fluorescent fatty-acid binding proteins present throughout the Anguilliformes order, common-name eels. Chlopsid FP is one novel fluorescent fatty acid-binding protein, which has been isolated from the Kaupichthys hyoproroides eel. In this study Chlopsid FP is further characterized by looking at its ability to bind its ligand bilirubin, through a point mutation study. By expanding the characterization of Chlopsid FP the significance of a glycine-proline-proline loop in fluorophore formation is confirmed. Furthermore, several specimens of eels are identified as being fluorescent, including a moray eel, Gymnothorax zonipectis. In doing so fluorescent proteins are implicated as the cause of fluorescence in several new eel specimens, expanding on the current understanding of fluorescent FABPs.In addition, Synodontidae, commonly called Lizardfish, a family of fish displaying both red and green fluorescence, are examined further. Until recently Lizardfish have been overlooked as potential keys to mechanistically understanding fluorescence. Using RNA-Seq technology and analyses, including transcriptomic annotation and differential expression, the Lizardfish family of fish is examined. Specifically, Saurida gracilis and Synodus lucioceps, and their associated fluorescent moieties are studied. The Saurida gracilis is examined due its dual fluorescence, as it expresses both red and green fluorescence. The Synodus lucioceps was selected as a vividly green fluorescent sample. Through examining both species a greater understanding of the Synodontidae family of fish is gained. Furthermore, using different approaches to examine each fish has allowed for the development of an efficient pathway to isolate and identify novel fluorescent moieties from marine organisms. Finally, two shark species, Cephaloscyllium ventriosum and Scyliorhinus retifer, are examined and characterized as cartilaginous fish expressing green fluorescence. A series of novel fluorescent metabolites are identified as small molecules responsible for fluorescence in both species. Additionally, the fluorescent properties of these metabolites, including excitation and emission spectra, are characterized. Ultimately, leading to the identification of a bromo-kynurenine metabolic pathway, furthering the current understanding of fluorescent small molecules.
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