東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Evaluation of Telomere Length in Spermatozoa as a Diagnostic Tool for Male Factor Infertility.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Evaluation of Telomere Length in Spermatozoa as a Diagnostic Tool for Male Factor Infertility./
作者:	Kurjanowicz, Pamela.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
面頁冊數:	249 p.
附註:	Source: Dissertations Abstracts International, Volume: 84-02, Section: B.
Contained By:	Dissertations Abstracts International84-02B.
標題:	Medicine. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29069642
ISBN:	9798834077435

Evaluation of Telomere Length in Spermatozoa as a Diagnostic Tool for Male Factor Infertility.
Kurjanowicz, Pamela.

Evaluation of Telomere Length in Spermatozoa as a Diagnostic Tool for Male Factor Infertility. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 249 p.

Source: Dissertations Abstracts International, Volume: 84-02, Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2022.

This item must not be sold to any third party vendors.

Over forty years since its inception, in vitro fertilization (IVF) has revolutionized the treatment of infertility (Edwards & Steptoe, 1980). The efficacy of IVF to treat severe male factor infertility, however, remains low due to the considerable knowledge gap in our understanding of its underlying etiology (Bernardini, 2000; Durak Aras et al., 2012; Tan et al., 1992). Clinical tests that hold predictive value towards pregnancy outcomes are still needed. In the last decade, the relationship between telomere length in sperm and male infertility has generated numerous confounding publications. In my research, I found that the effect size of sperm telomere length on fertility parameters (i.e., male age, sperm concentration, etc.) was relatively small; therefore, differences in study design moderator variables introduced sampling error and decreased the sensitivity of detection in previous publications. I also demonstrated that gold standard methods for telomere length determination were not applicable in sperm because the genome contained two distinct populations of telomeric DNA - telomeres on chromosome ends and free-floating extrachromosomal telomeric DNA repeats. I then applied the haloFISH assay, which measured the percentage of single sperm with truncated telomeres in individual patients, and confirmed that the presence of extrachromosomal telomeric DNA was a normal physiological characteristic of sperm, and that men who had shorter average sperm chromosomal telomere lengths had higher telomere length heterogeneity between sperm cells; an effect that is indicative of telomere crisis in somatic cells (Lansdorp, 1996; F. Wang et al., 2013). Given the preliminary sample size, the study was not adequately powered to detect a significant difference in chromosomal telomere length between groups, but a 'medium' effect size warrants further investigation to determine whether the length of telomeres in sperm can serve as a predictive clinical test for patients with male factor infertility.

ISBN: 9798834077435Subjects--Topical Terms:

641104
Medicine.
Subjects--Index Terms:

Spermatozoa

Evaluation of Telomere Length in Spermatozoa as a Diagnostic Tool for Male Factor Infertility.
LDR:03065nmm a2200313 4500 001 2352838
005 20221205085619.5
008 241004s2022 ||||||||||||||||| ||eng d
020 $a 9798834077435
035 $a (MiAaPQ)AAI29069642
035 $a AAI29069642
040 $a MiAaPQ $c MiAaPQ
100 1 $a Kurjanowicz, Pamela. $3 3692499
245 1 0 $a Evaluation of Telomere Length in Spermatozoa as a Diagnostic Tool for Male Factor Infertility.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2022
300 $a 249 p.
500 $a Source: Dissertations Abstracts International, Volume: 84-02, Section: B.
500 $a Advisor: Librach, Clifford.
502 $a Thesis (Ph.D.)--University of Toronto (Canada), 2022.
506 $a This item must not be sold to any third party vendors.
520 $a Over forty years since its inception, in vitro fertilization (IVF) has revolutionized the treatment of infertility (Edwards & Steptoe, 1980). The efficacy of IVF to treat severe male factor infertility, however, remains low due to the considerable knowledge gap in our understanding of its underlying etiology (Bernardini, 2000; Durak Aras et al., 2012; Tan et al., 1992). Clinical tests that hold predictive value towards pregnancy outcomes are still needed. In the last decade, the relationship between telomere length in sperm and male infertility has generated numerous confounding publications. In my research, I found that the effect size of sperm telomere length on fertility parameters (i.e., male age, sperm concentration, etc.) was relatively small; therefore, differences in study design moderator variables introduced sampling error and decreased the sensitivity of detection in previous publications. I also demonstrated that gold standard methods for telomere length determination were not applicable in sperm because the genome contained two distinct populations of telomeric DNA - telomeres on chromosome ends and free-floating extrachromosomal telomeric DNA repeats. I then applied the haloFISH assay, which measured the percentage of single sperm with truncated telomeres in individual patients, and confirmed that the presence of extrachromosomal telomeric DNA was a normal physiological characteristic of sperm, and that men who had shorter average sperm chromosomal telomere lengths had higher telomere length heterogeneity between sperm cells; an effect that is indicative of telomere crisis in somatic cells (Lansdorp, 1996; F. Wang et al., 2013). Given the preliminary sample size, the study was not adequately powered to detect a significant difference in chromosomal telomere length between groups, but a 'medium' effect size warrants further investigation to determine whether the length of telomeres in sperm can serve as a predictive clinical test for patients with male factor infertility.
590 $a School code: 0779.
650 4 $a Medicine. $3 641104
653 $a Spermatozoa
653 $a Male infertility
690 $a 0564
710 2 $a University of Toronto (Canada). $b Physiology. $3 3285885
773 0 $t Dissertations Abstracts International $g 84-02B.
790 $a 0779
791 $a Ph.D.
792 $a 2022
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29069642