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Laser Induced Differentiation of Human Adipose Derived Stem Cells to Functional Fibroblasts and Chondrocytes of the Temporomandibular Joint Disc - an in Vitro Study.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Laser Induced Differentiation of Human Adipose Derived Stem Cells to Functional Fibroblasts and Chondrocytes of the Temporomandibular Joint Disc - an in Vitro Study./
Author:	Karic, Vesna.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	134 p.
Notes:	Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
Contained By:	Dissertations Abstracts International83-05B.
Subject:	Tissue engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28701213
ISBN:	9798544211044

Laser Induced Differentiation of Human Adipose Derived Stem Cells to Functional Fibroblasts and Chondrocytes of the Temporomandibular Joint Disc - an in Vitro Study.
Karic, Vesna.

Laser Induced Differentiation of Human Adipose Derived Stem Cells to Functional Fibroblasts and Chondrocytes of the Temporomandibular Joint Disc - an in Vitro Study. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 134 p.

Source: Dissertations Abstracts International, Volume: 83-05, Section: B.

Thesis (D.Tech.)--University of Johannesburg (South Africa), 2021.

This item must not be sold to any third party vendors.

Temporomandibular disorders (TMD) are defined as a group of disorders of the temporomandibular joint (TMJ) and its musculature (Durham and Wassel, 2011). The treatment of degenerative changes in TMJ disc and TMD is challenging with no permanent solution. The population of fibroblasts and chondrocytes in TMJ disc has provided a hope to treat this disorder using modern techniques (Maenpaa et al., 2010). Photobiomodulation and stem cell therapy have emerged as novel treatments for TMD and degenerative changes of the TMJ disc (Zhu Xu et al., 2018, Zhang et al., 2015). ADSCs have been proven to have the capacity to differentiate into many cell types and tissues (Mazini 2019). Research has shown that photobiomodulation increases stem cell proliferation (Mvula et al., 2010; Mvula and Abrahamse, 2014). Stem cell therapy has been used in an attempt to replace degenerative TMJ disc cells (Zhang et al., 2015). However, despite significant improvements in the search for new treatments to improve TMDs, conclusive evidence of possible treatment regimes for degeneration of the TMJ disc remains to be discovered.The objective of this study was to explore the effect of laser irradiation with 660 and 940 nm diode lasers in differentiating ADSCs into fibroblasts and chondrocytes. The laser irradiation with/without basic fibroblast growth factor (bFGF) was used to seek permanent treatment of degenerative TMJ disc. The study used immortal ADSCs purchased from ATCC for all experiments. The ADSCs were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% foetal bovine serum, antibiotic and antimycotic supplements (Zuk et al., 2001). The cultures were then incubated at 37°C in a humidified atmosphere of 5% carbon dioxide (CO2).The ADSCs were harvested at a confluence not more than 90% and the viable cell numbers were quantified using an Invitrogen Countess™ II FL automated cell counter for seeding calculations. The morphology and cell growth were observed in a 3,4 cm diameter plate with 2 ml of complete v media at time intervals of 24, 48, 72 h; and 1-week and 2, 3-weeks. The optimized ADSCs in each group were irradiated with 660 nm and 940 nm diode lasers with/without basic fibroblast growth factor (bFGF) at 5 J/cm2 fluence, to induce differentiation into fibroblasts and chondrocytes. The bFGF is a known strong mitogen for many cell types (Nukavarapu et al., 2015; Desai et al., 2014). However, the use of bFGF was omitted in the differentiation of chondrocytes to avoid the interference of fibroblasts in culture. Hence, the significance of the laser alone in differentiation was determined.The experimental groups were divided into four: Group 1 (C) was a control group of ADSCs with no laser irradiation and basic fibroblast growth factor (bFGF) added. Group 2 (LB) with bFGF added at 10 ng/ml, before irradiation. Group 3 (B) with (10 ng/ml) bFGF alone and ADSCs exposed to laser irradiation alone, group 4 (L). Semiconfluent monolayer ADSCs were exposed to laser irradiation at 5 J/cm2 fluence with 660 nm and 940 nm diode lasers in separate experiments.

ISBN: 9798544211044Subjects--Topical Terms:

823582
Tissue engineering.

Laser Induced Differentiation of Human Adipose Derived Stem Cells to Functional Fibroblasts and Chondrocytes of the Temporomandibular Joint Disc - an in Vitro Study.
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520 $a Temporomandibular disorders (TMD) are defined as a group of disorders of the temporomandibular joint (TMJ) and its musculature (Durham and Wassel, 2011). The treatment of degenerative changes in TMJ disc and TMD is challenging with no permanent solution. The population of fibroblasts and chondrocytes in TMJ disc has provided a hope to treat this disorder using modern techniques (Maenpaa et al., 2010). Photobiomodulation and stem cell therapy have emerged as novel treatments for TMD and degenerative changes of the TMJ disc (Zhu Xu et al., 2018, Zhang et al., 2015). ADSCs have been proven to have the capacity to differentiate into many cell types and tissues (Mazini 2019). Research has shown that photobiomodulation increases stem cell proliferation (Mvula et al., 2010; Mvula and Abrahamse, 2014). Stem cell therapy has been used in an attempt to replace degenerative TMJ disc cells (Zhang et al., 2015). However, despite significant improvements in the search for new treatments to improve TMDs, conclusive evidence of possible treatment regimes for degeneration of the TMJ disc remains to be discovered.The objective of this study was to explore the effect of laser irradiation with 660 and 940 nm diode lasers in differentiating ADSCs into fibroblasts and chondrocytes. The laser irradiation with/without basic fibroblast growth factor (bFGF) was used to seek permanent treatment of degenerative TMJ disc. The study used immortal ADSCs purchased from ATCC for all experiments. The ADSCs were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% foetal bovine serum, antibiotic and antimycotic supplements (Zuk et al., 2001). The cultures were then incubated at 37°C in a humidified atmosphere of 5% carbon dioxide (CO2).The ADSCs were harvested at a confluence not more than 90% and the viable cell numbers were quantified using an Invitrogen Countess™ II FL automated cell counter for seeding calculations. The morphology and cell growth were observed in a 3,4 cm diameter plate with 2 ml of complete v media at time intervals of 24, 48, 72 h; and 1-week and 2, 3-weeks. The optimized ADSCs in each group were irradiated with 660 nm and 940 nm diode lasers with/without basic fibroblast growth factor (bFGF) at 5 J/cm2 fluence, to induce differentiation into fibroblasts and chondrocytes. The bFGF is a known strong mitogen for many cell types (Nukavarapu et al., 2015; Desai et al., 2014). However, the use of bFGF was omitted in the differentiation of chondrocytes to avoid the interference of fibroblasts in culture. Hence, the significance of the laser alone in differentiation was determined.The experimental groups were divided into four: Group 1 (C) was a control group of ADSCs with no laser irradiation and basic fibroblast growth factor (bFGF) added. Group 2 (LB) with bFGF added at 10 ng/ml, before irradiation. Group 3 (B) with (10 ng/ml) bFGF alone and ADSCs exposed to laser irradiation alone, group 4 (L). Semiconfluent monolayer ADSCs were exposed to laser irradiation at 5 J/cm2 fluence with 660 nm and 940 nm diode lasers in separate experiments.
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