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Production and characterization of monoclonal antibodies against infectious bursal disease virus and studies on antigenicity and immunogenicity of the virus.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Production and characterization of monoclonal antibodies against infectious bursal disease virus and studies on antigenicity and immunogenicity of the virus./
作者:	Tsai, Hsiang-Jung.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 1991,
面頁冊數:	128 p.
附註:	Source: Dissertations Abstracts International, Volume: 53-07, Section: B.
Contained By:	Dissertations Abstracts International53-07B.
標題:	Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9201766
ISBN:	9798207733760

Production and characterization of monoclonal antibodies against infectious bursal disease virus and studies on antigenicity and immunogenicity of the virus.
Tsai, Hsiang-Jung.

Production and characterization of monoclonal antibodies against infectious bursal disease virus and studies on antigenicity and immunogenicity of the virus. - Ann Arbor : ProQuest Dissertations & Theses, 1991 - 128 p.

Source: Dissertations Abstracts International, Volume: 53-07, Section: B.

Thesis (Ph.D.)--The Ohio State University, 1991.

This item must not be sold to any third party vendors.

A panel of hybridomas secreting monoclonal antibodies (MAbs) against infectious bursal disease virus (IBDV) were generated and characterized. All the non-neutralizing MAbs were found to bind to VP-3 of IBDV in a western blotting assay. These MAbs were shown to cross react with serotypes and subtypes of IBDV by indirect immunofluorescence assay, ELISA, and western blotting assay. One of the non-neutralizing MAb (26E10) was used in an immunoperoxidase (IP) staining technique to detect IBDV in the bursal samples of experimentally infected birds. The results were satisfactory when compared to virus isolation and dot-blot hybridization results. Yet, the IP staining method using the MAb is a simpler and more time economic diagnostic method compared to the virus isolation approach. A neutralizing MAb (33E8) was generated and characterized. MAb 33E8 neutralized both cell-adapted "standard" and "variant" strains of serotype 1 IBDVs in vitro at a similar titer. However, it did not neutralize serotype 2 IBDV. This indicated the epitope which MAb 33E8 against probably is a serotype 1 IBDV specific antigen. MAb 33E8 did not bind to any separated IBDV polypeptides in Western blotting assay. Thus the neutralizing epitope which MAb 33E8 against probably is conformational. Two variant strains of IBDV (IN and E) were adapted and passaged on the BGM-70 cell line for 30 and 40 times. Passage in tissue culture resulted in loss of pathogenicity. However, both viruses maintained their antigenicities as demonstrated by two in vitro tests (immunofluorescence test and neutralization test) and two in vivo experiments (inactivated preparation of both viruses induced satisfactory protection in vaccinated SPF chickens). The passaged viruses also became poor immunogens, since no protection was induced when the viruses were given to SPF chickens as live vaccines. It seems likely that the passaged viruses might have lost the ability to replicate in their natural host.

ISBN: 9798207733760Subjects--Topical Terms:

536250
Microbiology.
Subjects--Index Terms:

chickens

Production and characterization of monoclonal antibodies against infectious bursal disease virus and studies on antigenicity and immunogenicity of the virus.
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520 $a A panel of hybridomas secreting monoclonal antibodies (MAbs) against infectious bursal disease virus (IBDV) were generated and characterized. All the non-neutralizing MAbs were found to bind to VP-3 of IBDV in a western blotting assay. These MAbs were shown to cross react with serotypes and subtypes of IBDV by indirect immunofluorescence assay, ELISA, and western blotting assay. One of the non-neutralizing MAb (26E10) was used in an immunoperoxidase (IP) staining technique to detect IBDV in the bursal samples of experimentally infected birds. The results were satisfactory when compared to virus isolation and dot-blot hybridization results. Yet, the IP staining method using the MAb is a simpler and more time economic diagnostic method compared to the virus isolation approach. A neutralizing MAb (33E8) was generated and characterized. MAb 33E8 neutralized both cell-adapted "standard" and "variant" strains of serotype 1 IBDVs in vitro at a similar titer. However, it did not neutralize serotype 2 IBDV. This indicated the epitope which MAb 33E8 against probably is a serotype 1 IBDV specific antigen. MAb 33E8 did not bind to any separated IBDV polypeptides in Western blotting assay. Thus the neutralizing epitope which MAb 33E8 against probably is conformational. Two variant strains of IBDV (IN and E) were adapted and passaged on the BGM-70 cell line for 30 and 40 times. Passage in tissue culture resulted in loss of pathogenicity. However, both viruses maintained their antigenicities as demonstrated by two in vitro tests (immunofluorescence test and neutralization test) and two in vivo experiments (inactivated preparation of both viruses induced satisfactory protection in vaccinated SPF chickens). The passaged viruses also became poor immunogens, since no protection was induced when the viruses were given to SPF chickens as live vaccines. It seems likely that the passaged viruses might have lost the ability to replicate in their natural host.
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