東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Newly recognized rat parvoviruses: Characterization and diagnostic assay development.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Newly recognized rat parvoviruses: Characterization and diagnostic assay development./
作者:	Wan, Cho-Hua.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2001,
面頁冊數:	90 p.
附註:	Source: Dissertations Abstracts International, Volume: 63-04, Section: B.
Contained By:	Dissertations Abstracts International63-04B.
標題:	Veterinary services. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3013040
ISBN:	9780493233772

Newly recognized rat parvoviruses: Characterization and diagnostic assay development.
Wan, Cho-Hua.

Newly recognized rat parvoviruses: Characterization and diagnostic assay development. - Ann Arbor : ProQuest Dissertations & Theses, 2001 - 90 p.

Source: Dissertations Abstracts International, Volume: 63-04, Section: B.

Thesis (Ph.D.)--University of Missouri - Columbia, 2001.

This item must not be sold to any third party vendors.

Three rat parvoviruses, rat minute virus 1a, b, c (RMV-1a, b, c), distinct from Kilham rat virus (KRV), Toolan's H-1 parvovirus (H-1), and rat parvovirus 1a (RPV-1a), have been identified in naturally infected rats of four origin. The viral DNA genome sequences of three RMV-1 variants have been determined. Comparisons of nucleotide and amino acid sequences revealed the three RMV-1 variants are closely related to each other, are distinct but closely related to KRV and H-1, and are significantly different from RPV-1a. Two diagnostic PCR assays to specifically detect RMV-1 and RPV-1 were developed. These two PCR assays were shown to be sensitive, specific and rapid methods to detect RMV-1 and RPV-1 infections in laboratory rats. These assays may also be used to detect RMV-1 and RPV-1 contamination of cell lines and other biological samples. An enzyme-linked immunosorbent assay (ELISA) that uses recombinant capsid protein of RMV-1 as an antigen has been developed to detect antibodies in RMV-1 infected rats. The cDNA of the major capsid protein (VP2) was cloned from RMV-1 infected tissues, expressed in insect cells and used to develop an ELISA (RMV rVP2 ELISA). Comparison of the RMV rVP2 ELISA with the rNS1 ELISA indicated that the RMV rVP2 ELISA was more sensitive and specific for detection of RMV-1 antibodies.

ISBN: 9780493233772Subjects--Topical Terms:

3433982
Veterinary services.
Subjects--Index Terms:

Antibodies

Newly recognized rat parvoviruses: Characterization and diagnostic assay development.
LDR:02578nmm a2200373 4500 001 2349435
005 20220920134315.5
008 241004s2001 ||||||||||||||||| ||eng d
020 $a 9780493233772
035 $a (MiAaPQ)AAI3013040
035 $a AAI3013040
040 $a MiAaPQ $c MiAaPQ
100 1 $a Wan, Cho-Hua. $3 3688848
245 1 0 $a Newly recognized rat parvoviruses: Characterization and diagnostic assay development.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2001
300 $a 90 p.
500 $a Source: Dissertations Abstracts International, Volume: 63-04, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Riley, Lela K.
502 $a Thesis (Ph.D.)--University of Missouri - Columbia, 2001.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a Three rat parvoviruses, rat minute virus 1a, b, c (RMV-1a, b, c), distinct from Kilham rat virus (KRV), Toolan's H-1 parvovirus (H-1), and rat parvovirus 1a (RPV-1a), have been identified in naturally infected rats of four origin. The viral DNA genome sequences of three RMV-1 variants have been determined. Comparisons of nucleotide and amino acid sequences revealed the three RMV-1 variants are closely related to each other, are distinct but closely related to KRV and H-1, and are significantly different from RPV-1a. Two diagnostic PCR assays to specifically detect RMV-1 and RPV-1 were developed. These two PCR assays were shown to be sensitive, specific and rapid methods to detect RMV-1 and RPV-1 infections in laboratory rats. These assays may also be used to detect RMV-1 and RPV-1 contamination of cell lines and other biological samples. An enzyme-linked immunosorbent assay (ELISA) that uses recombinant capsid protein of RMV-1 as an antigen has been developed to detect antibodies in RMV-1 infected rats. The cDNA of the major capsid protein (VP2) was cloned from RMV-1 infected tissues, expressed in insect cells and used to develop an ELISA (RMV rVP2 ELISA). Comparison of the RMV rVP2 ELISA with the rNS1 ELISA indicated that the RMV rVP2 ELISA was more sensitive and specific for detection of RMV-1 antibodies.
590 $a School code: 0133.
650 4 $a Veterinary services. $3 3433982
650 4 $a Molecular biology. $3 517296
653 $a Antibodies
653 $a Capsid protein
653 $a Diagnostic assay
653 $a Parvoviruses
690 $a 0778
690 $a 0307
710 2 $a University of Missouri - Columbia. $3 1017522
773 0 $t Dissertations Abstracts International $g 63-04B.
790 $a 0133
791 $a Ph.D.
792 $a 2001
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3013040