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The Effect of Probiotic Isolates on Pathogenic Bacteria.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Effect of Probiotic Isolates on Pathogenic Bacteria./
Author:	Ellis, Christi.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
Description:	83 p.
Notes:	Source: Masters Abstracts International, Volume: 83-12.
Contained By:	Masters Abstracts International83-12.
Subject:	Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29210334
ISBN:	9798834020776

The Effect of Probiotic Isolates on Pathogenic Bacteria.
Ellis, Christi.

The Effect of Probiotic Isolates on Pathogenic Bacteria. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 83 p.

Source: Masters Abstracts International, Volume: 83-12.

Thesis (M.S.)--Southeast Missouri State University, 2022.

This item must not be sold to any third party vendors.

The objective of this study was to see if there was evidence to support the idea that probiotic bacteria help prevent the growth and colonization of pathogenic bacteria in the human digestive system. Nine organisms were isolated from consumer-available probiotic sources. Through various biochemical and molecular approaches, seven of the isolates were identified as being from the phylum Firmicutes, one from the phylum Actinobacteria, and one from the phylum Bacteroidota. These probiotic isolates were cultured with two attenuated pathogenic bacteria, Escherichia coli and Salmonella enterica, to assess the probiotics' effect on two common human digestive system pathogens. Under the conditions of these competition studies, the ANOVA test showed that the probiotic treatments lead to no statistically significant reductions in pathogenic bacteria growth. However, the ad hoc test, Mann-Whitney U, showed that there were significant increases and decreases in growth of some of the probiotic and pathogenic bacteria. The media used was effective at measuring the individual bacterial populations from mixed broth cultures. The potential for the isolates to function as probiotic organisms was discussed. Both the isolated probiotic organisms and the culturing techniques are of considerable interest for further investigation.

ISBN: 9798834020776Subjects--Topical Terms:

536250
Microbiology.
Subjects--Index Terms:

Escherichia coli

The Effect of Probiotic Isolates on Pathogenic Bacteria.
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500 $a Source: Masters Abstracts International, Volume: 83-12.
500 $a Advisor: Champine, James.
502 $a Thesis (M.S.)--Southeast Missouri State University, 2022.
506 $a This item must not be sold to any third party vendors.
520 $a The objective of this study was to see if there was evidence to support the idea that probiotic bacteria help prevent the growth and colonization of pathogenic bacteria in the human digestive system. Nine organisms were isolated from consumer-available probiotic sources. Through various biochemical and molecular approaches, seven of the isolates were identified as being from the phylum Firmicutes, one from the phylum Actinobacteria, and one from the phylum Bacteroidota. These probiotic isolates were cultured with two attenuated pathogenic bacteria, Escherichia coli and Salmonella enterica, to assess the probiotics' effect on two common human digestive system pathogens. Under the conditions of these competition studies, the ANOVA test showed that the probiotic treatments lead to no statistically significant reductions in pathogenic bacteria growth. However, the ad hoc test, Mann-Whitney U, showed that there were significant increases and decreases in growth of some of the probiotic and pathogenic bacteria. The media used was effective at measuring the individual bacterial populations from mixed broth cultures. The potential for the isolates to function as probiotic organisms was discussed. Both the isolated probiotic organisms and the culturing techniques are of considerable interest for further investigation.
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650 4 $a Biology. $3 522710
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653 $a Escherichia coli
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710 2 $a Southeast Missouri State University. $b College of Science, Technology, & Agriculture. $3 3346549
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