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The Effect of Induced Changes in gut Microbiota on Fish Performance.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Effect of Induced Changes in gut Microbiota on Fish Performance./
作者:	Deng, Yale.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
面頁冊數:	205 p.
附註:	Source: Dissertations Abstracts International, Volume: 84-01, Section: B.
Contained By:	Dissertations Abstracts International84-01B.
標題:	Aquaculture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29185646
ISBN:	9798835552931

The Effect of Induced Changes in gut Microbiota on Fish Performance.
Deng, Yale.

The Effect of Induced Changes in gut Microbiota on Fish Performance. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 205 p.

Source: Dissertations Abstracts International, Volume: 84-01, Section: B.

Thesis (Ph.D.)--Wageningen University and Research, 2022.

This item must not be sold to any third party vendors.

Fish-microbe interactions play an important role in the feed digestion, nutrient absorption, immune system development and disease susceptibility of the host. Therefore, a healthy and stable gut microbiota contributes to the growth and overall health status of the host. In aquaculture, practices like disinfection, water exchange, fish transfer, change in diet composition or feeding regime, and fluctuations in water quality affect fish microbiota. To improve the microbial management in aquaculture, it is important to know the main the factors governing fish gut microbiota development and to understand to which extent the induced changes in gut microbiota composition affect fish performance.Apart from host selection, water and feed are two main sources for the microbial colonisation of the intestinal tract of fish. Therefore, the aim of this thesis was to modulate fish gut microbiota by culturing the fish under different rearing conditions and by dietary supplementation of feed additives, with the expectation to improve the growth of the fish and gut microbiota homeostasis.The recent development and challenges faced by the aquaculture industry are summarized in Chapter 1. To improve the productivity and sustainability of aquaculture, the importance of environment-microbes-host interactions is highlighted. Furthermore, the factors mainly affecting fish gut microbiota composition and dynamics are reviewed, providing potential strategies to modulate fish gut microbiota. Besides, the analytical method of gut microbiota and the model species used are described. This chapter ends with an overview of the experimental work done during the thesis.In Chapter 2, the effect of different rearing conditions on the initial colonisation of the fish gut microbiota was investigated. To do so, Nile tilapia embryos were incubated and the hatched larva were cultured for 26 days in a flow-through system (FTS) or a recirculating aquaculture system (RAS). In addition, a treatment in which the probiotic Bacillus subtilis was administrated through the diet in RAS (RASB) was also examined. We show that larvae reared in FTS showed significantly lower survival than those in RAS and RASB, while no differences were observed in fish growth and the apparent feed conversion ratio between treatments. Different treatments resulted in different gut microbiota compositions, which strongly correlated with the survival rate and standard body length at harvest. Our findings indicate that survival of Nile tilapia larvae in RAS is higher than in FTS, while dietary probiotic supplementation further stimulates the presence of beneficial bacteria during early life.The growth-promoting effect of biofloc might be due to its nutritional value or its probiotic effect. To test this hypothesis, in Chapter 3 the effect of in-situ biofloc (LW) and dietary supplementation of ex-situ live (LF) or dead biofloc (DF) on fish gut microbiota composition and growth performance was compared to a biofloc-free control treatment. We identified a core gut prokaryotic community among all treatments. The relative abundance of the core community was significantly influenced by LW treatment, while the DF or LF treatments only caused minor changes on taxa not belonging to the core gut microbiota. The distinct microbial community in the biofloc water was associated with the modulatory effect of LW on tilapia gut microbiota. A growth-promoting effect on tilapia was observed from the LW treatment, while DF and LF treatments had no effect on fish growth performance as compared to the control treatment. Our study highlights the probiotic effect of in-situ biofloc water, in contrast to processed biofloc which showed little nutritional or probiotic effect.In addition to test the effect of different rearing environments on fish gut microbiota composition and fish performance in the previous chapters, Chapter 4 was designed to verify how dietary supplementation with probiotics and enzymes influence the nutrient digestibility and volatile fatty acid (VFA) content along the gastrointestinal tract and the microbial composition in the distal gut of juvenile Nile tilapia.

ISBN: 9798835552931Subjects--Topical Terms:

545878
Aquaculture.

The Effect of Induced Changes in gut Microbiota on Fish Performance.
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