東華大學圖書館 |

The Effect of Induced Changes in gut Microbiota on Fish Performance.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Effect of Induced Changes in gut Microbiota on Fish Performance./
Author:	Deng, Yale.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
Description:	205 p.
Notes:	Source: Dissertations Abstracts International, Volume: 84-01, Section: B.
Contained By:	Dissertations Abstracts International84-01B.
Subject:	Aquaculture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29185646
ISBN:	9798835552931

The Effect of Induced Changes in gut Microbiota on Fish Performance.
Deng, Yale.

The Effect of Induced Changes in gut Microbiota on Fish Performance. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 205 p.

Source: Dissertations Abstracts International, Volume: 84-01, Section: B.

Thesis (Ph.D.)--Wageningen University and Research, 2022.

This item must not be sold to any third party vendors.

Fish-microbe interactions play an important role in the feed digestion, nutrient absorption, immune system development and disease susceptibility of the host. Therefore, a healthy and stable gut microbiota contributes to the growth and overall health status of the host. In aquaculture, practices like disinfection, water exchange, fish transfer, change in diet composition or feeding regime, and fluctuations in water quality affect fish microbiota. To improve the microbial management in aquaculture, it is important to know the main the factors governing fish gut microbiota development and to understand to which extent the induced changes in gut microbiota composition affect fish performance.Apart from host selection, water and feed are two main sources for the microbial colonisation of the intestinal tract of fish. Therefore, the aim of this thesis was to modulate fish gut microbiota by culturing the fish under different rearing conditions and by dietary supplementation of feed additives, with the expectation to improve the growth of the fish and gut microbiota homeostasis.The recent development and challenges faced by the aquaculture industry are summarized in Chapter 1. To improve the productivity and sustainability of aquaculture, the importance of environment-microbes-host interactions is highlighted. Furthermore, the factors mainly affecting fish gut microbiota composition and dynamics are reviewed, providing potential strategies to modulate fish gut microbiota. Besides, the analytical method of gut microbiota and the model species used are described. This chapter ends with an overview of the experimental work done during the thesis.In Chapter 2, the effect of different rearing conditions on the initial colonisation of the fish gut microbiota was investigated. To do so, Nile tilapia embryos were incubated and the hatched larva were cultured for 26 days in a flow-through system (FTS) or a recirculating aquaculture system (RAS). In addition, a treatment in which the probiotic Bacillus subtilis was administrated through the diet in RAS (RASB) was also examined. We show that larvae reared in FTS showed significantly lower survival than those in RAS and RASB, while no differences were observed in fish growth and the apparent feed conversion ratio between treatments. Different treatments resulted in different gut microbiota compositions, which strongly correlated with the survival rate and standard body length at harvest. Our findings indicate that survival of Nile tilapia larvae in RAS is higher than in FTS, while dietary probiotic supplementation further stimulates the presence of beneficial bacteria during early life.The growth-promoting effect of biofloc might be due to its nutritional value or its probiotic effect. To test this hypothesis, in Chapter 3 the effect of in-situ biofloc (LW) and dietary supplementation of ex-situ live (LF) or dead biofloc (DF) on fish gut microbiota composition and growth performance was compared to a biofloc-free control treatment. We identified a core gut prokaryotic community among all treatments. The relative abundance of the core community was significantly influenced by LW treatment, while the DF or LF treatments only caused minor changes on taxa not belonging to the core gut microbiota. The distinct microbial community in the biofloc water was associated with the modulatory effect of LW on tilapia gut microbiota. A growth-promoting effect on tilapia was observed from the LW treatment, while DF and LF treatments had no effect on fish growth performance as compared to the control treatment. Our study highlights the probiotic effect of in-situ biofloc water, in contrast to processed biofloc which showed little nutritional or probiotic effect.In addition to test the effect of different rearing environments on fish gut microbiota composition and fish performance in the previous chapters, Chapter 4 was designed to verify how dietary supplementation with probiotics and enzymes influence the nutrient digestibility and volatile fatty acid (VFA) content along the gastrointestinal tract and the microbial composition in the distal gut of juvenile Nile tilapia.

ISBN: 9798835552931Subjects--Topical Terms:

545878
Aquaculture.

The Effect of Induced Changes in gut Microbiota on Fish Performance.
LDR:05361nmm a2200409 4500 001 2348289
005 20220908123022.5
008 241004s2022 ||||||||||||||||| ||eng d
020 $a 9798835552931
035 $a (MiAaPQ)AAI29185646
035 $a (MiAaPQ)Wageningen595620
035 $a AAI29185646
040 $a MiAaPQ $c MiAaPQ
100 1 $a Deng, Yale. $3 3687620
245 1 4 $a The Effect of Induced Changes in gut Microbiota on Fish Performance.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2022
300 $a 205 p.
500 $a Source: Dissertations Abstracts International, Volume: 84-01, Section: B.
500 $a Advisor: Verdegem, M. ;Kokou, F. .
502 $a Thesis (Ph.D.)--Wageningen University and Research, 2022.
506 $a This item must not be sold to any third party vendors.
520 $a Fish-microbe interactions play an important role in the feed digestion, nutrient absorption, immune system development and disease susceptibility of the host. Therefore, a healthy and stable gut microbiota contributes to the growth and overall health status of the host. In aquaculture, practices like disinfection, water exchange, fish transfer, change in diet composition or feeding regime, and fluctuations in water quality affect fish microbiota. To improve the microbial management in aquaculture, it is important to know the main the factors governing fish gut microbiota development and to understand to which extent the induced changes in gut microbiota composition affect fish performance.Apart from host selection, water and feed are two main sources for the microbial colonisation of the intestinal tract of fish. Therefore, the aim of this thesis was to modulate fish gut microbiota by culturing the fish under different rearing conditions and by dietary supplementation of feed additives, with the expectation to improve the growth of the fish and gut microbiota homeostasis.The recent development and challenges faced by the aquaculture industry are summarized in Chapter 1. To improve the productivity and sustainability of aquaculture, the importance of environment-microbes-host interactions is highlighted. Furthermore, the factors mainly affecting fish gut microbiota composition and dynamics are reviewed, providing potential strategies to modulate fish gut microbiota. Besides, the analytical method of gut microbiota and the model species used are described. This chapter ends with an overview of the experimental work done during the thesis.In Chapter 2, the effect of different rearing conditions on the initial colonisation of the fish gut microbiota was investigated. To do so, Nile tilapia embryos were incubated and the hatched larva were cultured for 26 days in a flow-through system (FTS) or a recirculating aquaculture system (RAS). In addition, a treatment in which the probiotic Bacillus subtilis was administrated through the diet in RAS (RASB) was also examined. We show that larvae reared in FTS showed significantly lower survival than those in RAS and RASB, while no differences were observed in fish growth and the apparent feed conversion ratio between treatments. Different treatments resulted in different gut microbiota compositions, which strongly correlated with the survival rate and standard body length at harvest. Our findings indicate that survival of Nile tilapia larvae in RAS is higher than in FTS, while dietary probiotic supplementation further stimulates the presence of beneficial bacteria during early life.The growth-promoting effect of biofloc might be due to its nutritional value or its probiotic effect. To test this hypothesis, in Chapter 3 the effect of in-situ biofloc (LW) and dietary supplementation of ex-situ live (LF) or dead biofloc (DF) on fish gut microbiota composition and growth performance was compared to a biofloc-free control treatment. We identified a core gut prokaryotic community among all treatments. The relative abundance of the core community was significantly influenced by LW treatment, while the DF or LF treatments only caused minor changes on taxa not belonging to the core gut microbiota. The distinct microbial community in the biofloc water was associated with the modulatory effect of LW on tilapia gut microbiota. A growth-promoting effect on tilapia was observed from the LW treatment, while DF and LF treatments had no effect on fish growth performance as compared to the control treatment. Our study highlights the probiotic effect of in-situ biofloc water, in contrast to processed biofloc which showed little nutritional or probiotic effect.In addition to test the effect of different rearing environments on fish gut microbiota composition and fish performance in the previous chapters, Chapter 4 was designed to verify how dietary supplementation with probiotics and enzymes influence the nutrient digestibility and volatile fatty acid (VFA) content along the gastrointestinal tract and the microbial composition in the distal gut of juvenile Nile tilapia.
590 $a School code: 2157.
650 4 $a Aquaculture. $3 545878
650 4 $a Cod. $3 3685254
650 4 $a Feeds. $3 659935
650 4 $a Food. $3 551593
650 4 $a Probiotics. $3 1532006
650 4 $a Zebrafish. $3 3685278
650 4 $a Disease. $3 705846
650 4 $a Nutrition research. $3 3564650
650 4 $a Antibiotics. $3 670318
650 4 $a Mortality. $3 533218
650 4 $a Carbohydrates. $3 522687
650 4 $a Immune system. $3 689864
650 4 $a Bacteria. $3 550366
650 4 $a Diet. $3 626002
650 4 $a Biomass. $3 1013462
650 4 $a Carp. $3 3687621
650 4 $a Enzymes. $3 520899
650 4 $a Microorganisms. $3 666946
650 4 $a Metabolites. $3 683644
650 4 $a Nitrogen. $3 1314426
650 4 $a Fish. $3 3564374
650 4 $a Alternative medicine. $3 536195
650 4 $a Animal sciences. $3 3174829
650 4 $a Aquatic sciences. $3 3174300
650 4 $a Biological oceanography. $3 2122748
650 4 $a Biology. $3 522710
650 4 $a Immunology. $3 611031
650 4 $a Medicine. $3 641104
650 4 $a Microbiology. $3 536250
650 4 $a Nutrition. $3 517777
650 4 $a Pharmaceutical sciences. $3 3173021
690 $a 0496
690 $a 0475
690 $a 0792
690 $a 0416
690 $a 0306
690 $a 0982
690 $a 0564
690 $a 0410
690 $a 0570
690 $a 0572
710 2 $a Wageningen University and Research. $3 3557914
773 0 $t Dissertations Abstracts International $g 84-01B.
790 $a 2157
791 $a Ph.D.
792 $a 2022
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29185646