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Characterization of Exocellular DNA in the Oligotrophic Ocean.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Characterization of Exocellular DNA in the Oligotrophic Ocean./
作者:	Linney, Morgan Donerly.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	166 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-04, Section: B.
Contained By:	Dissertations Abstracts International83-04B.
標題:	Biological oceanography. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28644792
ISBN:	9798471106970

Characterization of Exocellular DNA in the Oligotrophic Ocean.
Linney, Morgan Donerly.

Characterization of Exocellular DNA in the Oligotrophic Ocean. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 166 p.

Source: Dissertations Abstracts International, Volume: 83-04, Section: B.

Thesis (Ph.D.)--University of Hawai'i at Manoa, 2021.

This item must not be sold to any third party vendors.

Approximately half of the DNA in the open ocean is present outside of living cells. Together, this exocellular DNA (or dissolved DNA; D-DNA) is comprised of truly dissolved "free" DNA (F-DNA), virion encapsidated DNA, and DNA inside of membrane vesicles. It is ubiquitous in nature yet its sources, sinks, and ecological characteristics are largely unknown. One reason for the uncertainty is the methodological limitation that precludes a distinction among the three pools that comprise D-DNA. Using a novel method that provides complete separation of vesicles, viruses, and F-DNA, the first fully sequenced open ocean water-column profile of exocellular DNA was obtained. Euphotic zone F-DNA (75-125 m) contained mostly bacterial and viral sequences, with bacteria dominating in the mesopelagic zone (500-1000 m). A high proportion of mesopelagic zone (500 and 1000 m) F-DNA sequences appeared to originate from surface waters, including a large amount of DNA contributed by high-light Prochlorococcus ecotypes. These results indicate the composition of F-DNA in different regions of the water-column (euphotic and mesopelagic) and suggest potential mechanisms for dissolved organic matter cycling and export. Experiments designed to examine the dynamics of F-DNA suggest that it is produced by viral lysis of microbial cells, and may be consumed by heterotrophic bacteria and protists. Collectively, this research provides novel insights into the microbial origins and dynamics of F-DNA in the open ocean.

ISBN: 9798471106970Subjects--Topical Terms:

2122748
Biological oceanography.
Subjects--Index Terms:

Dissolved organic carbon

Characterization of Exocellular DNA in the Oligotrophic Ocean.
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520 $a Approximately half of the DNA in the open ocean is present outside of living cells. Together, this exocellular DNA (or dissolved DNA; D-DNA) is comprised of truly dissolved "free" DNA (F-DNA), virion encapsidated DNA, and DNA inside of membrane vesicles. It is ubiquitous in nature yet its sources, sinks, and ecological characteristics are largely unknown. One reason for the uncertainty is the methodological limitation that precludes a distinction among the three pools that comprise D-DNA. Using a novel method that provides complete separation of vesicles, viruses, and F-DNA, the first fully sequenced open ocean water-column profile of exocellular DNA was obtained. Euphotic zone F-DNA (75-125 m) contained mostly bacterial and viral sequences, with bacteria dominating in the mesopelagic zone (500-1000 m). A high proportion of mesopelagic zone (500 and 1000 m) F-DNA sequences appeared to originate from surface waters, including a large amount of DNA contributed by high-light Prochlorococcus ecotypes. These results indicate the composition of F-DNA in different regions of the water-column (euphotic and mesopelagic) and suggest potential mechanisms for dissolved organic matter cycling and export. Experiments designed to examine the dynamics of F-DNA suggest that it is produced by viral lysis of microbial cells, and may be consumed by heterotrophic bacteria and protists. Collectively, this research provides novel insights into the microbial origins and dynamics of F-DNA in the open ocean.
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