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Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli./
Author:	Gargatte, Sweta.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	39 p.
Notes:	Source: Masters Abstracts International, Volume: 83-02.
Contained By:	Masters Abstracts International83-02.
Subject:	Chemical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28322846
ISBN:	9798534660159

Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli.
Gargatte, Sweta.

Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 39 p.

Source: Masters Abstracts International, Volume: 83-02.

Thesis (M.S.)--Rutgers The State University of New Jersey, School of Graduate Studies, 2021.

This item must not be sold to any third party vendors.

4-hydroxystyrene is a phenolic chemical compound used in polymer and flavor industries. Microbial biosynthesis of this compound has been studied over recent years as a sustainable green alternative to synthetic chemical production. In this current study, 4-hydroxystyrene was biosynthesized using engineered E. coli. To improve the hydroxystyrene biosynthesis utilizing the carbon source following strategies were adopted. The pathway for bioproduction was bifurcated, and the co-culture of E. coli was implemented over monoculture. An amino acid exporter protein PhpCAT was utilized in combination biosensor-assisted cell selection mechanism to maximize the pathway intermediate tyrosine's biosynthesis. The exporter improved tyrosine's mass transfer between the co-culture members, and the resulting co-culture produced 298 mg/L 4-hydroxystyrene from 5 g/L glucose, which is 96% higher than the control co-culture without the exporter. The achieved yield of 0.06 g/g glucose is the highest among previous studies on de novo microbial biosynthesis. It shows that the exporter protein's utilization and biosensors enhance the pathway intermediate in a co-culture study and boost the hydroxystyrene biosynthesis performance. This strategy can pave the path for microbial biosynthesis for all the potential aromatic-derived compounds.

ISBN: 9798534660159Subjects--Topical Terms:

560457
Chemical engineering.
Subjects--Index Terms:

Amino acid exporter

Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli.
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260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 39 p.
500 $a Source: Masters Abstracts International, Volume: 83-02.
500 $a Advisor: Zhang, Haoran.
502 $a Thesis (M.S.)--Rutgers The State University of New Jersey, School of Graduate Studies, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a 4-hydroxystyrene is a phenolic chemical compound used in polymer and flavor industries. Microbial biosynthesis of this compound has been studied over recent years as a sustainable green alternative to synthetic chemical production. In this current study, 4-hydroxystyrene was biosynthesized using engineered E. coli. To improve the hydroxystyrene biosynthesis utilizing the carbon source following strategies were adopted. The pathway for bioproduction was bifurcated, and the co-culture of E. coli was implemented over monoculture. An amino acid exporter protein PhpCAT was utilized in combination biosensor-assisted cell selection mechanism to maximize the pathway intermediate tyrosine's biosynthesis. The exporter improved tyrosine's mass transfer between the co-culture members, and the resulting co-culture produced 298 mg/L 4-hydroxystyrene from 5 g/L glucose, which is 96% higher than the control co-culture without the exporter. The achieved yield of 0.06 g/g glucose is the highest among previous studies on de novo microbial biosynthesis. It shows that the exporter protein's utilization and biosensors enhance the pathway intermediate in a co-culture study and boost the hydroxystyrene biosynthesis performance. This strategy can pave the path for microbial biosynthesis for all the potential aromatic-derived compounds.
590 $a School code: 0190.
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650 4 $a Microbiology. $3 536250
650 4 $a Food science. $3 3173303
650 4 $a Polymer chemistry. $3 3173488
650 4 $a Biochemistry. $3 518028
650 4 $a Biosynthesis. $3 516407
650 4 $a Amino acids. $3 558768
650 4 $a Ratios. $3 3561161
650 4 $a E coli. $3 3556834
650 4 $a Metabolism. $3 541349
650 4 $a Kinases. $3 3558077
650 4 $a Proteins. $3 558769
650 4 $a Plasmids. $3 3319070
650 4 $a Gene expression. $3 643979
650 4 $a Glycerol. $3 3685180
650 4 $a Hypotheses. $3 3560118
650 4 $a Trace elements. $3 602273
650 4 $a Experiments. $3 525909
650 4 $a Carbon. $3 604057
650 4 $a Solvents. $3 620946
650 4 $a Sensors. $3 3549539
650 4 $a Glucose. $3 2084335
650 4 $a Engineering. $3 586835
650 4 $a Biosensors. $3 621001
650 4 $a Enzymes. $3 520899
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653 $a Metabolite
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