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A New Definition and Classification of Antibody Complementarity Determining Regions: Unsupervised Learning of Protein Backbone Conformations Informs Antibody Structural Bioinformatics and Design.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A New Definition and Classification of Antibody Complementarity Determining Regions: Unsupervised Learning of Protein Backbone Conformations Informs Antibody Structural Bioinformatics and Design./
Author:	Kelow, Simon Paul.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	169 p.
Notes:	Source: Dissertations Abstracts International, Volume: 83-03, Section: A.
Contained By:	Dissertations Abstracts International83-03A.
Subject:	Biophysics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28651122
ISBN:	9798535590899

A New Definition and Classification of Antibody Complementarity Determining Regions: Unsupervised Learning of Protein Backbone Conformations Informs Antibody Structural Bioinformatics and Design.
Kelow, Simon Paul.

A New Definition and Classification of Antibody Complementarity Determining Regions: Unsupervised Learning of Protein Backbone Conformations Informs Antibody Structural Bioinformatics and Design. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 169 p.

Source: Dissertations Abstracts International, Volume: 83-03, Section: A.

Thesis (Ph.D.)--University of Pennsylvania, 2021.

This item must not be sold to any third party vendors.

One of the main challenges in modern molecular biology is to establish general, robust, and precise descriptions of the relationship between structural features of molecules (DNA, RNA, proteins, and glycans) and the sequence of their constituent chemical building blocks (nucleotides, amino acids, monosachharides). In his 1951 Nobel lecture, Linus Pauling predicted that chemistry of the future would rely upon these descriptions to solve problems in biological medicine relevant to human health. As of July 8, 2021, X-ray crystallography, NMR, and Cryo-EM have solved 179,842 molecular structures, which have been deposited in the Protein Data Bank (PDB) along with their associated sequences. Antibodies are the largest such family of deposited protein structures in the PDB, and their importance to human health and research in molecular biology is widely acknowledged. In this work, I first show the development and validation of unsupervised learning software to cluster protein backbone conformations (clustering of backbones for Ramachandran analysis, or COBRA). I then describe the application of this software to the wealth of antibody data in the PDB to provide a novel, electron density validated classification of the antibody complementarity determining regions (CDRs). I compare this new classification to previous classifications of the CDRs to show the improvement of the association between the sequences and structures of the CDRs, the ability to robustly separate various CDR families, and the ability to assess the confidence in the quality of CDR families using electron density as support. In addition to providing a new classification of the antibody CDRs by clustering their backbone conformations, I provide an expanded definition of the antibody binding region by defining, naming, and classifying an antibody V-region segment named the "DE loop", which resembles the other six CDRs in sequence and structural variability, ability to bind antigen, and ability to stabilize antibodies, but has no current recognition as a canonical member of the CDRs. Finally, I show examples implementing these analyses in RosettaAntibodyDesign (RAbD) software to design antibodies towards SARS-COV-2 Spike Protein Type 1 (S1) Receptor Binding Domain (RBD), and show the experimental data for the generated antibody designs.

ISBN: 9798535590899Subjects--Topical Terms:

518360
Biophysics.
Subjects--Index Terms:

Antibody classification

A New Definition and Classification of Antibody Complementarity Determining Regions: Unsupervised Learning of Protein Backbone Conformations Informs Antibody Structural Bioinformatics and Design.
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300 $a 169 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-03, Section: A.
500 $a Advisor: Dunbrack, Roland L.;Sharp, Kim A.
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520 $a One of the main challenges in modern molecular biology is to establish general, robust, and precise descriptions of the relationship between structural features of molecules (DNA, RNA, proteins, and glycans) and the sequence of their constituent chemical building blocks (nucleotides, amino acids, monosachharides). In his 1951 Nobel lecture, Linus Pauling predicted that chemistry of the future would rely upon these descriptions to solve problems in biological medicine relevant to human health. As of July 8, 2021, X-ray crystallography, NMR, and Cryo-EM have solved 179,842 molecular structures, which have been deposited in the Protein Data Bank (PDB) along with their associated sequences. Antibodies are the largest such family of deposited protein structures in the PDB, and their importance to human health and research in molecular biology is widely acknowledged. In this work, I first show the development and validation of unsupervised learning software to cluster protein backbone conformations (clustering of backbones for Ramachandran analysis, or COBRA). I then describe the application of this software to the wealth of antibody data in the PDB to provide a novel, electron density validated classification of the antibody complementarity determining regions (CDRs). I compare this new classification to previous classifications of the CDRs to show the improvement of the association between the sequences and structures of the CDRs, the ability to robustly separate various CDR families, and the ability to assess the confidence in the quality of CDR families using electron density as support. In addition to providing a new classification of the antibody CDRs by clustering their backbone conformations, I provide an expanded definition of the antibody binding region by defining, naming, and classifying an antibody V-region segment named the "DE loop", which resembles the other six CDRs in sequence and structural variability, ability to bind antigen, and ability to stabilize antibodies, but has no current recognition as a canonical member of the CDRs. Finally, I show examples implementing these analyses in RosettaAntibodyDesign (RAbD) software to design antibodies towards SARS-COV-2 Spike Protein Type 1 (S1) Receptor Binding Domain (RBD), and show the experimental data for the generated antibody designs.
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650 4 $a Bioinformatics. $3 553671
650 4 $a Molecular physics. $3 3174737
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650 4 $a Datasets. $3 3541416
650 4 $a Hydrogen bonds. $3 768332
650 4 $a Mutation. $3 837917
650 4 $a Cysts. $3 3683664
650 4 $a Binding sites. $3 3560349
650 4 $a Amino acids. $3 558768
650 4 $a Clustering. $3 3559215
650 4 $a Peptides. $3 605772
650 4 $a Mechanics. $3 525881
650 4 $a Drug dosages. $3 3557732
650 4 $a Proteins. $3 558769
650 4 $a Monte Carlo simulation. $3 3564916
650 4 $a Liver. $3 3564653
650 4 $a Medical research. $2 bicssc $3 1556686
650 4 $a Classification. $3 595585
650 4 $a Insects. $3 516323
650 4 $a Design. $3 518875
650 4 $a Ligands. $3 686413
650 4 $a Antigens. $3 700737
650 4 $a Algorithms. $3 536374
650 4 $a Homocysteine. $3 3302341
650 4 $a Enzymes. $3 520899
650 4 $a Statistical methods. $3 731085
653 $a Antibody classification
653 $a Antibody design
653 $a Backbone conformation clustering
653 $a SARS-COV-2
653 $a Structural bioinformatics
653 $a Unsupervised machine learning
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