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DNA-Origami-based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.

Record Type:	Electronic resources : Monograph/item
Title/Author:	DNA-Origami-based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells./
Author:	Williams, Nathan David.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	92 p.
Notes:	Source: Dissertations Abstracts International, Volume: 83-02, Section: A.
Contained By:	Dissertations Abstracts International83-02A.
Subject:	Nanotechnology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28313950
ISBN:	9798522948092

DNA-Origami-based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.
Williams, Nathan David.

DNA-Origami-based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 92 p.

Source: Dissertations Abstracts International, Volume: 83-02, Section: A.

Thesis (Ph.D.)--Yale University, 2021.

This item must not be sold to any third party vendors.

Fluorescence microscopy has been one of the most discovery-rich methods in biology. In the digital age, the discipline is becoming increasingly quantitative. Virtually all biological laboratories have access to fluorescence microscopes, but abilities to quantify biomolecule copy numbers are limited by the complexity and sophistication associated with current quantification methods. Here, we present DNA-origami-based fluorescence brightness standards for counting 5-300 copies of proteins in bacterial and mammalian cells, tagged with fluorescent proteins or membrane-permeable organic dyes. Compared to conventional quantification techniques, our brightness standards are robust, straightforward to use, and compatible with nearly all fluorescence imaging applications, thereby providing a practical and versatile tool to quantify biomolecules via fluorescence microscopy.

ISBN: 9798522948092Subjects--Topical Terms:

526235
Nanotechnology.
Subjects--Index Terms:

Conjugation

DNA-Origami-based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.
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245 1 0 $a DNA-Origami-based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 92 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-02, Section: A.
500 $a Advisor: Lin, Chenxiang.
502 $a Thesis (Ph.D.)--Yale University, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a Fluorescence microscopy has been one of the most discovery-rich methods in biology. In the digital age, the discipline is becoming increasingly quantitative. Virtually all biological laboratories have access to fluorescence microscopes, but abilities to quantify biomolecule copy numbers are limited by the complexity and sophistication associated with current quantification methods. Here, we present DNA-origami-based fluorescence brightness standards for counting 5-300 copies of proteins in bacterial and mammalian cells, tagged with fluorescent proteins or membrane-permeable organic dyes. Compared to conventional quantification techniques, our brightness standards are robust, straightforward to use, and compatible with nearly all fluorescence imaging applications, thereby providing a practical and versatile tool to quantify biomolecules via fluorescence microscopy.
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650 4 $a Calibration. $3 2068745
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650 4 $a Amino acids. $3 558768
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