東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep./
作者:	Middleton, Denzel Deshawn.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	94 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
Contained By:	Dissertations Abstracts International83-05B.
標題:	Animal sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28811942
ISBN:	9798494452191

The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep.
Middleton, Denzel Deshawn.

The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 94 p.

Source: Dissertations Abstracts International, Volume: 83-05, Section: B.

Thesis (Ph.D.)--West Virginia University, 2021.

This item must not be sold to any third party vendors.

Little is known about differences observed in early immunological signaling events that result in cellular infiltration and Th2 cytokine production that mediate parasite expulsion. Parasite resistant St. Croix (STC) sheep have been used as model for full host protective immunity as they present the ability to respond to larval stages of H. contortus. RNA sequencing analysis of peripheral blood mononuclear cells (PBMC) stimulated with H. contortus larval antigen (HcLA) revealed significant upregulation in NLRP3 and its pathway component TLR4. Predicted protein structural software identified distinct differences in NLRP3 protein structure in STC and parasite susceptible Suffolk (SUF) sheep. NLRP3 has been widely studied in a classic model of inflammatory infection, but little is known about its role in helminth infections. The inhibition of NLRP3 in a classic model of infection reduced STC PBMC responses on both the transcript and protein level but had no effect on the transcript level for SUF PBMC only the protein level. In an HcLA infection model PBMC from SUF with the inhibition of NLRP3 resulted in the upregulation of TLR4, TLR2, and IL-10. We hypothesize NLRP3 loss of function to have a significant impact on components of TLR4 signaling and inflammatory immune activation to pathogenic challenge. The inhibition of a dysfunctional NLRP3 receptor in SUF sheep maybe a potential target for chemotherapeutics to achieve full host protective immunity.

ISBN: 9798494452191Subjects--Topical Terms:

3174829
Animal sciences.
Subjects--Index Terms:

Haemonchus contortus

The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep.
LDR:02792nmm a2200421 4500 001 2342432
005 20220318093143.5
008 241004s2021 ||||||||||||||||| ||eng d
020 $a 9798494452191
035 $a (MiAaPQ)AAI28811942
035 $a (MiAaPQ)WVirginia9253
035 $a AAI28811942
040 $a MiAaPQ $c MiAaPQ
100 1 $a Middleton, Denzel Deshawn. $3 3680810
245 1 4 $a The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 94 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
500 $a Advisor: Bowdridge, Scott;Yao, Jianbo;Schafer, Rosana.
502 $a Thesis (Ph.D.)--West Virginia University, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a Little is known about differences observed in early immunological signaling events that result in cellular infiltration and Th2 cytokine production that mediate parasite expulsion. Parasite resistant St. Croix (STC) sheep have been used as model for full host protective immunity as they present the ability to respond to larval stages of H. contortus. RNA sequencing analysis of peripheral blood mononuclear cells (PBMC) stimulated with H. contortus larval antigen (HcLA) revealed significant upregulation in NLRP3 and its pathway component TLR4. Predicted protein structural software identified distinct differences in NLRP3 protein structure in STC and parasite susceptible Suffolk (SUF) sheep. NLRP3 has been widely studied in a classic model of inflammatory infection, but little is known about its role in helminth infections. The inhibition of NLRP3 in a classic model of infection reduced STC PBMC responses on both the transcript and protein level but had no effect on the transcript level for SUF PBMC only the protein level. In an HcLA infection model PBMC from SUF with the inhibition of NLRP3 resulted in the upregulation of TLR4, TLR2, and IL-10. We hypothesize NLRP3 loss of function to have a significant impact on components of TLR4 signaling and inflammatory immune activation to pathogenic challenge. The inhibition of a dysfunctional NLRP3 receptor in SUF sheep maybe a potential target for chemotherapeutics to achieve full host protective immunity.
590 $a School code: 0256.
650 4 $a Animal sciences. $3 3174829
650 4 $a Bioinformatics. $3 553671
650 4 $a Cellular biology. $3 3172791
650 4 $a Chemistry. $3 516420
650 4 $a Genetics. $3 530508
650 4 $a Immunology. $3 611031
650 4 $a Medicine. $3 641104
650 4 $a Parasitology. $3 635062
653 $a Haemonchus contortus
653 $a Immune response
653 $a Parasite susceptiblility
690 $a 0475
690 $a 0715
690 $a 0379
690 $a 0485
690 $a 0369
690 $a 0982
690 $a 0564
690 $a 0718
710 2 $a West Virginia University. $3 1017532
773 0 $t Dissertations Abstracts International $g 83-05B.
790 $a 0256
791 $a Ph.D.
792 $a 2021
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28811942