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Protocols in biochemistry and clinic...

Pandey, Shweta.

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Protocols in biochemistry and clinical biochemistry

Record Type:	Electronic resources : Monograph/item
Title/Author:	Protocols in biochemistry and clinical biochemistry/ Shweta Pandey, Shyamal K. Goswami, Buddhi Prakash Jain.
Author:	Pandey, Shweta.
other author:	Goswami, Shyamal K.
Published:	Amsterdam :Academic Press, : 2021.,
Description:	1 online resource :col. ill.
[NT 15003449]:	Unit I: Buffer, solution and pH 1. Preparation of normal and molar solution 2. Buffer preparation 3. Determination of pKa, pH Unit II: Nucleic Acids 1. Isolation and Purification of DNA 2. Isolation and Purification of RNA 3. Purification of Nucleic Acid 4. Quantitative estimation of Nucleic acid 5. Agarose gel electrophoresis for separation of nucleic acid Unit III: Carbohydrate and Lipids 1. Qualitative test for carbohydrate (Benedict, Molisch etc) 2. Qualitative test for Lipids 3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids Unit III: Proteins 1. Isolation of Protein 2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc) 3. Estimation of Protein (Bradford, Lowry etc) 4. Paper Chromatography for separation and identification of amino acid 5. Ion exchange chromatography for separation of amino acid 6. Protein purification 7. SDS PAGE analysis of proteins 8. Western blotting for protein analysis 9. Isoelectric pH determination Unit IV: Enzymes 1. Enzyme kinetics 2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity 3. Enzymatic activity of Trypsin and Lipase 4. Enzyme purification 5. Determination of Km, Vmax and Ki Unit V: Clinical test 1. Acid and Alkaline phosphatase activity 2. SGPT and SGOT test 3. Estimation of Urea, Uric acid and Creatinine 4. Estimation of Glucose 5. Estimation of Cholesterol 6. Estimation of Vitamins 7. Glucose tolerance test 8. Estimation of Ca2+ and T4 in serum 9. Pregnancy test by HCG hormone 10. Estimation of Glycosylated haemoglobin.
Subject:	Biochemistry - Technique. -
Online resource:	https://www.sciencedirect.com/science/book/9780128220078
ISBN:	9780128220085 (electronic bk.)

Protocols in biochemistry and clinical biochemistry
Pandey, Shweta.

Protocols in biochemistry and clinical biochemistry[electronic resource] /Shweta Pandey, Shyamal K. Goswami, Buddhi Prakash Jain. - Amsterdam :Academic Press,2021. - 1 online resource :col. ill.

Includes bibliographical references and index.

Unit I: Buffer, solution and pH 1. Preparation of normal and molar solution 2. Buffer preparation 3. Determination of pKa, pH Unit II: Nucleic Acids 1. Isolation and Purification of DNA 2. Isolation and Purification of RNA 3. Purification of Nucleic Acid 4. Quantitative estimation of Nucleic acid 5. Agarose gel electrophoresis for separation of nucleic acid Unit III: Carbohydrate and Lipids 1. Qualitative test for carbohydrate (Benedict, Molisch etc) 2. Qualitative test for Lipids 3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids Unit III: Proteins 1. Isolation of Protein 2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc) 3. Estimation of Protein (Bradford, Lowry etc) 4. Paper Chromatography for separation and identification of amino acid 5. Ion exchange chromatography for separation of amino acid 6. Protein purification 7. SDS PAGE analysis of proteins 8. Western blotting for protein analysis 9. Isoelectric pH determination Unit IV: Enzymes 1. Enzyme kinetics 2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity 3. Enzymatic activity of Trypsin and Lipase 4. Enzyme purification 5. Determination of Km, Vmax and Ki Unit V: Clinical test 1. Acid and Alkaline phosphatase activity 2. SGPT and SGOT test 3. Estimation of Urea, Uric acid and Creatinine 4. Estimation of Glucose 5. Estimation of Cholesterol 6. Estimation of Vitamins 7. Glucose tolerance test 8. Estimation of Ca2+ and T4 in serum 9. Pregnancy test by HCG hormone 10. Estimation of Glycosylated haemoglobin.

ISBN: 9780128220085 (electronic bk.)

Nat. Bib. No.: GBC0E3371bnbSubjects--Topical Terms:

661280
Biochemistry
--Technique.Index Terms--Genre/Form:

542853
Electronic books.

LC Class. No.: QP519.7

Dewey Class. No.: 572

Protocols in biochemistry and clinical biochemistry
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020 $a 9780128220078 (pbk.)
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100 1 $a Pandey, Shweta. $3 3615077
245 1 0 $a Protocols in biochemistry and clinical biochemistry $h [electronic resource] / $c Shweta Pandey, Shyamal K. Goswami, Buddhi Prakash Jain.
260 $a Amsterdam : $b Academic Press, $c 2021.
300 $a 1 online resource : $b col. ill.
504 $a Includes bibliographical references and index.
505 0 $a Unit I: Buffer, solution and pH 1. Preparation of normal and molar solution 2. Buffer preparation 3. Determination of pKa, pH Unit II: Nucleic Acids 1. Isolation and Purification of DNA 2. Isolation and Purification of RNA 3. Purification of Nucleic Acid 4. Quantitative estimation of Nucleic acid 5. Agarose gel electrophoresis for separation of nucleic acid Unit III: Carbohydrate and Lipids 1. Qualitative test for carbohydrate (Benedict, Molisch etc) 2. Qualitative test for Lipids 3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids Unit III: Proteins 1. Isolation of Protein 2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc) 3. Estimation of Protein (Bradford, Lowry etc) 4. Paper Chromatography for separation and identification of amino acid 5. Ion exchange chromatography for separation of amino acid 6. Protein purification 7. SDS PAGE analysis of proteins 8. Western blotting for protein analysis 9. Isoelectric pH determination Unit IV: Enzymes 1. Enzyme kinetics 2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity 3. Enzymatic activity of Trypsin and Lipase 4. Enzyme purification 5. Determination of Km, Vmax and Ki Unit V: Clinical test 1. Acid and Alkaline phosphatase activity 2. SGPT and SGOT test 3. Estimation of Urea, Uric acid and Creatinine 4. Estimation of Glucose 5. Estimation of Cholesterol 6. Estimation of Vitamins 7. Glucose tolerance test 8. Estimation of Ca2+ and T4 in serum 9. Pregnancy test by HCG hormone 10. Estimation of Glycosylated haemoglobin.
650 0 $a Biochemistry $x Technique. $3 661280
655 4 $a Electronic books. $2 lcsh $3 542853
700 1 $a Goswami, Shyamal K. $3 3615078
700 1 $a Jain, Buddhi Prakash. $3 3615079
856 4 0 $u https://www.sciencedirect.com/science/book/9780128220078