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The Effects of Electrical and Physic...

Blatz, Joshua M.

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The Effects of Electrical and Physical Parameters on a Dielectric Barrier Discharge.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Effects of Electrical and Physical Parameters on a Dielectric Barrier Discharge./
Author:	Blatz, Joshua M.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	220 p.
Notes:	Source: Dissertations Abstracts International, Volume: 82-11, Section: B.
Contained By:	Dissertations Abstracts International82-11B.
Subject:	Applied physics. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28418365
ISBN:	9798708759016

The Effects of Electrical and Physical Parameters on a Dielectric Barrier Discharge.
Blatz, Joshua M.

The Effects of Electrical and Physical Parameters on a Dielectric Barrier Discharge. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 220 p.

Source: Dissertations Abstracts International, Volume: 82-11, Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2021.

This item must not be sold to any third party vendors.

Recently, promise has been shown for the use of non-thermal atmospheric-pressure plasmas in the fields of biology and medicine. One such technique is PLasma-Initiated Modification of Biomolecules or PLIMB, which uses hydroxyl radicals generated by a dielectric barrier discharge to label proteins in solution.The hypothesis of this work is that by optimizing the electrical and physical parameters, as well as gas composition of a dielectric-barrier discharge, hydroxyl-radical generation can be improved while minimizing detrimental change in quantities such as solution pH and temperature.The changes in electrical parameters include frequency (2 to 16 kHz), applied voltage (4 to 9 kV), voltage offset (-5 to +5 kV) and voltage waveform (sinusoid, triangle, square). Physical properties include the distance between the high-voltage electrode and the protein-containing solution surface (0.25 to 2.25 mm) as well as electrode shape. The composition of the gas (air, helium, nitrogen, argon) in which the plasma was generated was also varied. Electrical and spectrographic measurements were gathered and used as aids to understand the origin of these results.For electrical parameters, it was found that an increase in either frequency or voltage yielded an increase in hydroxyl-radical generation while voltage offset had little to no effect. Each of the three tested waveforms showed little difference in hydroxyl-radical generation for ten second exposures but square and sinusoidal waveforms generated more discharges.For physical parameters, an optimal distance of 0.75mm separating the high voltage electrode and the solution surface was found. Investigations into electrode shape showed that a pointed tip is useful for the consistent generation of plasma discharges, but finer pointed tips showed a reduction in hydroxyl-radical generation.For gas composition, air generated the largest number of hydroxyl radicals due to a larger presence of water vapor. It was also found that solution temperature and pH were least affected when using helium.From these results, numerous means of improving the rate of hydroxyl-radical generation have been identified. Additionally, conditions have been identified which can be useful for proteins that are more susceptible to perturbations due to heat or changes in pH.

ISBN: 9798708759016Subjects--Topical Terms:

3343996
Applied physics.
Subjects--Index Terms:

Atmospheric-Pressure Plasma

The Effects of Electrical and Physical Parameters on a Dielectric Barrier Discharge.
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245 1 4 $a The Effects of Electrical and Physical Parameters on a Dielectric Barrier Discharge.
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300 $a 220 p.
500 $a Source: Dissertations Abstracts International, Volume: 82-11, Section: B.
500 $a Advisor: Shohet, Juda L.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a Recently, promise has been shown for the use of non-thermal atmospheric-pressure plasmas in the fields of biology and medicine. One such technique is PLasma-Initiated Modification of Biomolecules or PLIMB, which uses hydroxyl radicals generated by a dielectric barrier discharge to label proteins in solution.The hypothesis of this work is that by optimizing the electrical and physical parameters, as well as gas composition of a dielectric-barrier discharge, hydroxyl-radical generation can be improved while minimizing detrimental change in quantities such as solution pH and temperature.The changes in electrical parameters include frequency (2 to 16 kHz), applied voltage (4 to 9 kV), voltage offset (-5 to +5 kV) and voltage waveform (sinusoid, triangle, square). Physical properties include the distance between the high-voltage electrode and the protein-containing solution surface (0.25 to 2.25 mm) as well as electrode shape. The composition of the gas (air, helium, nitrogen, argon) in which the plasma was generated was also varied. Electrical and spectrographic measurements were gathered and used as aids to understand the origin of these results.For electrical parameters, it was found that an increase in either frequency or voltage yielded an increase in hydroxyl-radical generation while voltage offset had little to no effect. Each of the three tested waveforms showed little difference in hydroxyl-radical generation for ten second exposures but square and sinusoidal waveforms generated more discharges.For physical parameters, an optimal distance of 0.75mm separating the high voltage electrode and the solution surface was found. Investigations into electrode shape showed that a pointed tip is useful for the consistent generation of plasma discharges, but finer pointed tips showed a reduction in hydroxyl-radical generation.For gas composition, air generated the largest number of hydroxyl radicals due to a larger presence of water vapor. It was also found that solution temperature and pH were least affected when using helium.From these results, numerous means of improving the rate of hydroxyl-radical generation have been identified. Additionally, conditions have been identified which can be useful for proteins that are more susceptible to perturbations due to heat or changes in pH.
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653 $a Protein Footprinting
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710 2 $a The University of Wisconsin - Madison. $b Electrical Engineering. $3 2095968
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856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28418365