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Characterization of Heavy Metal Resi...

Patel, Esha.

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Characterization of Heavy Metal Resistance Genes from a Cryptic Plasmid of Pseudomonas B0623, Isolated from the Deep Subsurface of Savannah River, Ga.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of Heavy Metal Resistance Genes from a Cryptic Plasmid of Pseudomonas B0623, Isolated from the Deep Subsurface of Savannah River, Ga./
Author:	Patel, Esha.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	38 p.
Notes:	Source: Masters Abstracts International, Volume: 82-01.
Contained By:	Masters Abstracts International82-01.
Subject:	Biology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27999191
ISBN:	9798661932518

Characterization of Heavy Metal Resistance Genes from a Cryptic Plasmid of Pseudomonas B0623, Isolated from the Deep Subsurface of Savannah River, Ga.
Patel, Esha.

Characterization of Heavy Metal Resistance Genes from a Cryptic Plasmid of Pseudomonas B0623, Isolated from the Deep Subsurface of Savannah River, Ga. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 38 p.

Source: Masters Abstracts International, Volume: 82-01.

Thesis (M.S.)--Adelphi University, 2020.

This item must not be sold to any third party vendors.

Pseudomonas sp. B0623 was obtained from the Savannah River Site, a nuclear reservation in South Carolina that is highly contaminated with heavy metals. The bacterial isolates are therefore expected to contain heavy metal resistance genes that allow them to survive in a contaminated environment. Among all those bacterial isolates, the strain B0623 was cultured, its plasmid DNA was extracted and purified using a plasmid extraction kit and long-read whole genome sequencing was performed using Pac-Bio sequencing. After quality control checks, assembly was performed using the program Canu within the Cyverse Discovery Environment, which allowed 7.2 million reads to be assembled into 20,636 contigs. Visualization of the resulting assembly graph in the program Bandage revealed that 3 plasmids were assembled precisely during assembly, and at least 5 plasmids were likely present in Pseudomonas sp B0623. CyVerse and RAST were used to annotate the largest plasmid, and the RAST output file was screened for presence of metal resistance genes.

ISBN: 9798661932518Subjects--Topical Terms:

522710
Biology.
Subjects--Index Terms:

Savannah River, Georgia

Characterization of Heavy Metal Resistance Genes from a Cryptic Plasmid of Pseudomonas B0623, Isolated from the Deep Subsurface of Savannah River, Ga.
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245 1 0 $a Characterization of Heavy Metal Resistance Genes from a Cryptic Plasmid of Pseudomonas B0623, Isolated from the Deep Subsurface of Savannah River, Ga.
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300 $a 38 p.
500 $a Source: Masters Abstracts International, Volume: 82-01.
500 $a Advisor: Coombs, Jonna.
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506 $a This item must not be sold to any third party vendors.
520 $a Pseudomonas sp. B0623 was obtained from the Savannah River Site, a nuclear reservation in South Carolina that is highly contaminated with heavy metals. The bacterial isolates are therefore expected to contain heavy metal resistance genes that allow them to survive in a contaminated environment. Among all those bacterial isolates, the strain B0623 was cultured, its plasmid DNA was extracted and purified using a plasmid extraction kit and long-read whole genome sequencing was performed using Pac-Bio sequencing. After quality control checks, assembly was performed using the program Canu within the Cyverse Discovery Environment, which allowed 7.2 million reads to be assembled into 20,636 contigs. Visualization of the resulting assembly graph in the program Bandage revealed that 3 plasmids were assembled precisely during assembly, and at least 5 plasmids were likely present in Pseudomonas sp B0623. CyVerse and RAST were used to annotate the largest plasmid, and the RAST output file was screened for presence of metal resistance genes.
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650 4 $a Limnology. $3 545788
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653 $a Heavy metals
653 $a Bacterial isolates
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773 0 $t Masters Abstracts International $g 82-01.
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