東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Evolution and Genetic Dissection of ...

Dew-Budd, Kelly.

Linked to FindBook

Google Book

Amazon

博客來

Evolution and Genetic Dissection of Two RNA-Dependent Processes in Brassicaceae.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Evolution and Genetic Dissection of Two RNA-Dependent Processes in Brassicaceae./
Author:	Dew-Budd, Kelly.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	97 p.
Notes:	Source: Dissertations Abstracts International, Volume: 82-05, Section: B.
Contained By:	Dissertations Abstracts International82-05B.
Subject:	Plant sciences. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28152705
ISBN:	9798691217012

Evolution and Genetic Dissection of Two RNA-Dependent Processes in Brassicaceae.
Dew-Budd, Kelly.

Evolution and Genetic Dissection of Two RNA-Dependent Processes in Brassicaceae. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 97 p.

Source: Dissertations Abstracts International, Volume: 82-05, Section: B.

Thesis (Ph.D.)--The University of Arizona, 2020.

This item must not be sold to any third party vendors.

The important role RNA plays in regulating and stabilizing genomes has garnered increased attention since the finding that the majority of the human genome is transcribed (1). However, determining which transcriptional products function in these roles, and which are transcriptional noise, has been challenging. A potential genomic watermark of loci that encode functional RNA is sequence conservation among related species, but the non-coding RNAs that have been characterized have highlighted the relaxed evolutionary constraints of functionally important non-coding RNAs. In some cases, the transcription of certain classes of non-coding RNAs are not in dispute, yet their functional importance remains elusive. To overcome these challenges researchers have begun to expand characterization of non-coding RNAs using a comparative framework, thereby connecting sequence conservation with functional conservation. In this dissertation, I expound on these solutions and use them to show the power of comparative evolutionary approaches when studying non-coding RNAs. First, I show the utility of CRISPR/Cas9 genome editing in generating mutants that permit the clear functional characterization of a long non-coding RNA locus whose identity was drawn into question because of lack of sequence conservation in critical domains. Then I describe a transformation method for the obligate outcrosser, Capsella grandiflora, that expands the genetic resources of the Brassicaceae available for comparative evolutionary analyses in the study of non-coding RNAs. Finally, I use a comparative approach to elucidate the spectrum of impacts resulting from alterations to the RNA-directed DNA Methylation (RdDM) pathway in three related species. More specifically, I probe the contributions of RdDM in mediating genomic conflicts during seed development. In sum, I demonstrate that the study of RNAs benefits from a multi-species approach, particularly when those species can be readily transformed.

ISBN: 9798691217012Subjects--Topical Terms:

3173832
Plant sciences.
Subjects--Index Terms:

Capsella grandiflora

Evolution and Genetic Dissection of Two RNA-Dependent Processes in Brassicaceae.
LDR:03244nmm a2200397 4500 001 2275857
005 20210401103754.5
008 220723s2020 ||||||||||||||||| ||eng d
020 $a 9798691217012
035 $a (MiAaPQ)AAI28152705
035 $a AAI28152705
040 $a MiAaPQ $c MiAaPQ
100 1 $a Dew-Budd, Kelly. $3 3554102
245 1 0 $a Evolution and Genetic Dissection of Two RNA-Dependent Processes in Brassicaceae.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 97 p.
500 $a Source: Dissertations Abstracts International, Volume: 82-05, Section: B.
500 $a Includes supplementary digital materials.
500 $a Advisor: Beilstein, Mark A.
502 $a Thesis (Ph.D.)--The University of Arizona, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a The important role RNA plays in regulating and stabilizing genomes has garnered increased attention since the finding that the majority of the human genome is transcribed (1). However, determining which transcriptional products function in these roles, and which are transcriptional noise, has been challenging. A potential genomic watermark of loci that encode functional RNA is sequence conservation among related species, but the non-coding RNAs that have been characterized have highlighted the relaxed evolutionary constraints of functionally important non-coding RNAs. In some cases, the transcription of certain classes of non-coding RNAs are not in dispute, yet their functional importance remains elusive. To overcome these challenges researchers have begun to expand characterization of non-coding RNAs using a comparative framework, thereby connecting sequence conservation with functional conservation. In this dissertation, I expound on these solutions and use them to show the power of comparative evolutionary approaches when studying non-coding RNAs. First, I show the utility of CRISPR/Cas9 genome editing in generating mutants that permit the clear functional characterization of a long non-coding RNA locus whose identity was drawn into question because of lack of sequence conservation in critical domains. Then I describe a transformation method for the obligate outcrosser, Capsella grandiflora, that expands the genetic resources of the Brassicaceae available for comparative evolutionary analyses in the study of non-coding RNAs. Finally, I use a comparative approach to elucidate the spectrum of impacts resulting from alterations to the RNA-directed DNA Methylation (RdDM) pathway in three related species. More specifically, I probe the contributions of RdDM in mediating genomic conflicts during seed development. In sum, I demonstrate that the study of RNAs benefits from a multi-species approach, particularly when those species can be readily transformed.
590 $a School code: 0009.
650 4 $a Plant sciences. $3 3173832
650 4 $a Genetics. $3 530508
650 4 $a Evolution & development. $3 3172418
653 $a Capsella grandiflora
653 $a Capsella rubella
653 $a CRISPR
653 $a Epigenetics
653 $a RNA-directed DNA methylation
653 $a Telomerase
690 $a 0479
690 $a 0369
690 $a 0412
710 2 $a The University of Arizona. $b Plant Science. $3 1677054
773 0 $t Dissertations Abstracts International $g 82-05B.
790 $a 0009
791 $a Ph.D.
792 $a 2020
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28152705