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Respiratory Chain Activity and Prote...

Alcorn de Feliz, Earsie L.

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Respiratory Chain Activity and Protein Expression in Skeletal Muscles from High and Low Feed Efficient Swine within the Same Genetic Line.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Respiratory Chain Activity and Protein Expression in Skeletal Muscles from High and Low Feed Efficient Swine within the Same Genetic Line./
Author:	Alcorn de Feliz, Earsie L.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	129 p.
Notes:	Source: Dissertations Abstracts International, Volume: 82-03, Section: B.
Contained By:	Dissertations Abstracts International82-03B.
Subject:	Animal sciences. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28030124
ISBN:	9798664733167

Respiratory Chain Activity and Protein Expression in Skeletal Muscles from High and Low Feed Efficient Swine within the Same Genetic Line.
Alcorn de Feliz, Earsie L.

Respiratory Chain Activity and Protein Expression in Skeletal Muscles from High and Low Feed Efficient Swine within the Same Genetic Line. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 129 p.

Source: Dissertations Abstracts International, Volume: 82-03, Section: B.

Thesis (Ph.D.)--University of Arkansas, 2020.

This item must not be sold to any third party vendors.

Current as well as past studies have provided evidence for a connection between macroeconomic/agricultural and cellular/biochemical basis of feed efficiency (FE; gain:feed) in farm animal production. Our investigation of respiratory chain activity, mitochondrial protein oxidation and protein expression was an expansion to the subcellular/molecular level. In this study we investigated the entire complex (Cx) of the electron transport chain (ETC) for differences in Cx activities in mitochondria isolated from skeletal muscle in high (HFE) and low (LFE) feed efficient swine within the same genetic line. Review of literature provided information for the connection between feed efficiency, mitochondrial function, and muscle type. Using UV spectrophotometry, gel electrophoresis and western blotting methods, we were able to study mitochondria from swine Longissimus (LM) and Spinalis dorsi muscles for differences in respiratory chain complex (I - V) activities, protein oxidation and protein expression of eleven subunits of the five electron transport chain complexes. We observed muscle dependent differences in Cx I and II, protein carbonyls and five subunits of the ETC (Cx I 30, Cor II, Cox IV, alpha and beta ATPase). We concluded after comparing our results with findings in literature, that respiratory chain activity protein oxidation and expression of protein subunits may be both skeletal muscle as well as a species dependent. These differential expressions may be the result of increased incidents of reactive oxygen species causing defects in Cx I or Cx II that may precipitate significant mitochondrial events and cellular pathways that provide links to the phenotypic expression of feed efficiency. Of high interest in relation to the mitochondrial link to FE are apoptotic, mTor and myostatin pathways.

ISBN: 9798664733167Subjects--Topical Terms:

3174829
Animal sciences.
Subjects--Index Terms:

Complex (Cx)

Respiratory Chain Activity and Protein Expression in Skeletal Muscles from High and Low Feed Efficient Swine within the Same Genetic Line.
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100 1 $a Alcorn de Feliz, Earsie L. $3 3554055
245 1 0 $a Respiratory Chain Activity and Protein Expression in Skeletal Muscles from High and Low Feed Efficient Swine within the Same Genetic Line.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 129 p.
500 $a Source: Dissertations Abstracts International, Volume: 82-03, Section: B.
500 $a Advisor: Maxwell, Charles V.
502 $a Thesis (Ph.D.)--University of Arkansas, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Current as well as past studies have provided evidence for a connection between macroeconomic/agricultural and cellular/biochemical basis of feed efficiency (FE; gain:feed) in farm animal production. Our investigation of respiratory chain activity, mitochondrial protein oxidation and protein expression was an expansion to the subcellular/molecular level. In this study we investigated the entire complex (Cx) of the electron transport chain (ETC) for differences in Cx activities in mitochondria isolated from skeletal muscle in high (HFE) and low (LFE) feed efficient swine within the same genetic line. Review of literature provided information for the connection between feed efficiency, mitochondrial function, and muscle type. Using UV spectrophotometry, gel electrophoresis and western blotting methods, we were able to study mitochondria from swine Longissimus (LM) and Spinalis dorsi muscles for differences in respiratory chain complex (I - V) activities, protein oxidation and protein expression of eleven subunits of the five electron transport chain complexes. We observed muscle dependent differences in Cx I and II, protein carbonyls and five subunits of the ETC (Cx I 30, Cor II, Cox IV, alpha and beta ATPase). We concluded after comparing our results with findings in literature, that respiratory chain activity protein oxidation and expression of protein subunits may be both skeletal muscle as well as a species dependent. These differential expressions may be the result of increased incidents of reactive oxygen species causing defects in Cx I or Cx II that may precipitate significant mitochondrial events and cellular pathways that provide links to the phenotypic expression of feed efficiency. Of high interest in relation to the mitochondrial link to FE are apoptotic, mTor and myostatin pathways.
590 $a School code: 0011.
650 4 $a Animal sciences. $3 3174829
650 4 $a Food science. $3 3173303
650 4 $a Physiology. $3 518431
650 4 $a Hogs. $3 3554056
650 4 $a Protein expression. $3 3554057
653 $a Complex (Cx)
653 $a Feed efficiency
653 $a Longissimus muscle (LM)
653 $a Mitochondria isolated
653 $a Respiratory chain Activity
653 $a Spinalis dorsi muscle (SpD)
690 $a 0475
690 $a 0359
690 $a 0719
710 2 $a University of Arkansas. $b Food Science. $3 3282884
773 0 $t Dissertations Abstracts International $g 82-03B.
790 $a 0011
791 $a Ph.D.
792 $a 2020
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28030124