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Investigation of Terminal Alkene For...

Blake-Hedges, Jacquelyn M.

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Investigation of Terminal Alkene Formation by Acyl-CoA Dehydrogenases in the Biosynthesis of Complex Natural Products.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Investigation of Terminal Alkene Formation by Acyl-CoA Dehydrogenases in the Biosynthesis of Complex Natural Products./
作者:	Blake-Hedges, Jacquelyn M.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	147 p.
附註:	Source: Dissertations Abstracts International, Volume: 81-10, Section: B.
Contained By:	Dissertations Abstracts International81-10B.
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27668068
ISBN:	9781658465212

Investigation of Terminal Alkene Formation by Acyl-CoA Dehydrogenases in the Biosynthesis of Complex Natural Products.
Blake-Hedges, Jacquelyn M.

Investigation of Terminal Alkene Formation by Acyl-CoA Dehydrogenases in the Biosynthesis of Complex Natural Products. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 147 p.

Source: Dissertations Abstracts International, Volume: 81-10, Section: B.

Thesis (Ph.D.)--University of California, Berkeley, 2019.

This item must not be sold to any third party vendors.

Polyketides are a class of natural products known for their chemical complexity and bioactive properties. They are biosynthesized by an assembly line-like system termed polyketide synthases (PKSs), in which each module consists of various enzymatic domains, each of which has a single catalytic function. The polyketide is extended by two carbons and selectively reduced by each module. These biosynthetic principles make PKSs attractive engineering targets, because a simple swap of one enzymatic domain could change the final polyketide structure and effect an improved or novel bioactivity. However, gaping holes in our understanding of polyketide biosynthesis still exist. In particular, the activities of polyketide-associated enzymes remain relatively under-characterized and are generally not the primary focus of engineering efforts. These enzymes, which include glycosyl transferases, cytochrome P450s, and many others, often are responsible for imparting bioactive functional groups into the polyketide backbone or after the assembly of the "naked" polyketide. Many of these non-canonical functional groups discovered within polyketides are therefore the most desirable engineering targets. In this dissertation, I describe my efforts to characterize a family of terminal alkene-forming enzymes which were originally identified in a polyketide biosynthetic cluster. I show that the enzymes are regioselective and that regioselectivity is controlled by a shift in the protein structure. Finally, I show that these enzymes are widespread in not only polyketide biosynthetic gene clusters, but also in other natural product clusters in the genomes of diverse Actinomycetes.

ISBN: 9781658465212Subjects--Topical Terms:

3172791
Cellular biology.
Subjects--Index Terms:

Terminal alkene formation

Investigation of Terminal Alkene Formation by Acyl-CoA Dehydrogenases in the Biosynthesis of Complex Natural Products.
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520 $a Polyketides are a class of natural products known for their chemical complexity and bioactive properties. They are biosynthesized by an assembly line-like system termed polyketide synthases (PKSs), in which each module consists of various enzymatic domains, each of which has a single catalytic function. The polyketide is extended by two carbons and selectively reduced by each module. These biosynthetic principles make PKSs attractive engineering targets, because a simple swap of one enzymatic domain could change the final polyketide structure and effect an improved or novel bioactivity. However, gaping holes in our understanding of polyketide biosynthesis still exist. In particular, the activities of polyketide-associated enzymes remain relatively under-characterized and are generally not the primary focus of engineering efforts. These enzymes, which include glycosyl transferases, cytochrome P450s, and many others, often are responsible for imparting bioactive functional groups into the polyketide backbone or after the assembly of the "naked" polyketide. Many of these non-canonical functional groups discovered within polyketides are therefore the most desirable engineering targets. In this dissertation, I describe my efforts to characterize a family of terminal alkene-forming enzymes which were originally identified in a polyketide biosynthetic cluster. I show that the enzymes are regioselective and that regioselectivity is controlled by a shift in the protein structure. Finally, I show that these enzymes are widespread in not only polyketide biosynthetic gene clusters, but also in other natural product clusters in the genomes of diverse Actinomycetes.
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