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A High-throughput Gain of Function S...

Dunn, Patrick James.

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A High-throughput Gain of Function Screen Identifies ABC Transporters as Modulators of VRAC-Dependent ATP Release.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A High-throughput Gain of Function Screen Identifies ABC Transporters as Modulators of VRAC-Dependent ATP Release./
Author:	Dunn, Patrick James.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	96 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-10, Section: B.
Contained By:	Dissertations Abstracts International81-10B.
Subject:	Cellular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27541619
ISBN:	9798607316365

A High-throughput Gain of Function Screen Identifies ABC Transporters as Modulators of VRAC-Dependent ATP Release.
Dunn, Patrick James.

A High-throughput Gain of Function Screen Identifies ABC Transporters as Modulators of VRAC-Dependent ATP Release. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 96 p.

Source: Dissertations Abstracts International, Volume: 81-10, Section: B.

Thesis (Ph.D.)--Yale University, 2019.

This item must not be sold to any third party vendors.

Cells can communicate with each other by releasing molecules into the extracellular space, where they can then bind to receptors and activate a host of cellular responses. This form of communication is involved in many important biological functions such as flow-induced vasodilation and synaptic transmission. Understanding how these chemical messengers are released, which receptors they bind to, and how those receptors are regulated will allow us to better understand human physiology and provide new drug targets for treating human disease.In this dissertation, we established and performed a high-throughput gain of function screen to identify new chemical release machinery, new receptors, and new functional modulators of neurotransmitter receptors. We accomplished this task by assembling and screening the largest ORF collection to date (>17,000 nonredundant genes, ~90% of all protein-coding genes). Through this process, we identified specific modulators of 13 different neurotransmitter receptors, and identified several ABC transporters, including ABCG1, as modulators of cholesterol-dependent ATP release through volume regulated anion channels (VRACs) in cerebellar granule neurons.

ISBN: 9798607316365Subjects--Topical Terms:

3172791
Cellular biology.
Subjects--Index Terms:

ABC transporters

A High-throughput Gain of Function Screen Identifies ABC Transporters as Modulators of VRAC-Dependent ATP Release.
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245 1 0 $a A High-throughput Gain of Function Screen Identifies ABC Transporters as Modulators of VRAC-Dependent ATP Release.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 96 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-10, Section: B.
500 $a Advisor: Tomita, Susumu.
502 $a Thesis (Ph.D.)--Yale University, 2019.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a Cells can communicate with each other by releasing molecules into the extracellular space, where they can then bind to receptors and activate a host of cellular responses. This form of communication is involved in many important biological functions such as flow-induced vasodilation and synaptic transmission. Understanding how these chemical messengers are released, which receptors they bind to, and how those receptors are regulated will allow us to better understand human physiology and provide new drug targets for treating human disease.In this dissertation, we established and performed a high-throughput gain of function screen to identify new chemical release machinery, new receptors, and new functional modulators of neurotransmitter receptors. We accomplished this task by assembling and screening the largest ORF collection to date (>17,000 nonredundant genes, ~90% of all protein-coding genes). Through this process, we identified specific modulators of 13 different neurotransmitter receptors, and identified several ABC transporters, including ABCG1, as modulators of cholesterol-dependent ATP release through volume regulated anion channels (VRACs) in cerebellar granule neurons.
590 $a School code: 0265.
650 4 $a Cellular biology. $3 3172791
650 4 $a Physiology. $3 518431
653 $a ABC transporters
690 $a 0379
690 $a 0719
710 2 $a Yale University. $b Cellular and Molecular Physiology. $3 2101656
773 0 $t Dissertations Abstracts International $g 81-10B.
790 $a 0265
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27541619