東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Investigating the Use of Human Monoc...

Zhang, Xiaoqing.

Linked to FindBook

Google Book

Amazon

博客來

Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration./
Author:	Zhang, Xiaoqing.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	252 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-05, Section: B.
Contained By:	Dissertations Abstracts International81-05B.
Subject:	Biomedical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=22587504
ISBN:	9781392732427

Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration.
Zhang, Xiaoqing.

Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 252 p.

Source: Dissertations Abstracts International, Volume: 81-05, Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2019.

This item must not be sold to any third party vendors.

In vascular tissue engineering, it is desirable to promote the proliferation and uniform distribution of the tissue-specific cells within a biomaterial scaffold, and to stimulate the cells to synthesize and deposit appropriate extracellular matrix (ECM). The ability to generate and accumulate tissue-specific ECM is especially important since the ECM not only provides structural support for the engineered tissue, but also directs signalling towards surrounding cells in order to initiate remodelling processes. However, currently there is limited ability to promote ECM production during in vitro vascular tissue engineering. Aside from the limited ECM-promoting strategies, another challenge that has been recognized is the lack of a vascular smooth muscle cell (VSMC) source that is effective, robust and safe. The thesis project investigated in vitro VSMC-monocyte co-culture systems. It was found that achieving a desired release profile of growth factors and hydrolytic proteases to direct ECM-promoting and ECM-degrading activities in the multi-cellular microenvironment is effective at promoting ECM accumulation during vascular tissue engineering. Additionally, adipose derived stromal cells (ASCs) were differentiated into VSMC-like cells. ASC-VSMC-monocyte co-culture systems were constructed to track the potential phenotypic changes of ASC-VSMCs (in order to further assess the feasibility of applying ASC-VSMCs as a replacement cell source for mature tissue VSMCs). The study demonstrated that the degradable polar hydrophobic ionic polyurethane (D-PHI) biomaterial scaffold based ASC-VSMC-monocyte co-culture system showed more release of proinflammatory cytokines at week 1 and 2. However, it showed more anti-inflammatory/wound-healing cytokine release at week 4. This study revealed important signalling factors and some mechanisms underlying phenotypic switches of ASC-VSMCs exposed to monocytes during in vitro tissue engineering processes. This can provide insights into the feasibility of ASC-VSMCs for vascular tissue development. Additionally, this work improved understanding of the balance between inflammation and wound-healing processes during tissue regeneration and remodeling using biomaterial scaffolds.

ISBN: 9781392732427Subjects--Topical Terms:

535387
Biomedical engineering.
Subjects--Index Terms:

Vascular smooth muscle cells

Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration.
LDR:03458nmm a2200337 4500 001 2272697
005 20201105110200.5
008 220629s2019 ||||||||||||||||| ||eng d
020 $a 9781392732427
035 $a (MiAaPQ)AAI22587504
035 $a AAI22587504
040 $a MiAaPQ $c MiAaPQ
100 1 $a Zhang, Xiaoqing. $3 3494925
245 1 0 $a Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 252 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-05, Section: B.
500 $a Advisor: Santerre, J. Paul;Simmons, Craig.
502 $a Thesis (Ph.D.)--University of Toronto (Canada), 2019.
506 $a This item must not be sold to any third party vendors.
520 $a In vascular tissue engineering, it is desirable to promote the proliferation and uniform distribution of the tissue-specific cells within a biomaterial scaffold, and to stimulate the cells to synthesize and deposit appropriate extracellular matrix (ECM). The ability to generate and accumulate tissue-specific ECM is especially important since the ECM not only provides structural support for the engineered tissue, but also directs signalling towards surrounding cells in order to initiate remodelling processes. However, currently there is limited ability to promote ECM production during in vitro vascular tissue engineering. Aside from the limited ECM-promoting strategies, another challenge that has been recognized is the lack of a vascular smooth muscle cell (VSMC) source that is effective, robust and safe. The thesis project investigated in vitro VSMC-monocyte co-culture systems. It was found that achieving a desired release profile of growth factors and hydrolytic proteases to direct ECM-promoting and ECM-degrading activities in the multi-cellular microenvironment is effective at promoting ECM accumulation during vascular tissue engineering. Additionally, adipose derived stromal cells (ASCs) were differentiated into VSMC-like cells. ASC-VSMC-monocyte co-culture systems were constructed to track the potential phenotypic changes of ASC-VSMCs (in order to further assess the feasibility of applying ASC-VSMCs as a replacement cell source for mature tissue VSMCs). The study demonstrated that the degradable polar hydrophobic ionic polyurethane (D-PHI) biomaterial scaffold based ASC-VSMC-monocyte co-culture system showed more release of proinflammatory cytokines at week 1 and 2. However, it showed more anti-inflammatory/wound-healing cytokine release at week 4. This study revealed important signalling factors and some mechanisms underlying phenotypic switches of ASC-VSMCs exposed to monocytes during in vitro tissue engineering processes. This can provide insights into the feasibility of ASC-VSMCs for vascular tissue development. Additionally, this work improved understanding of the balance between inflammation and wound-healing processes during tissue regeneration and remodeling using biomaterial scaffolds.
590 $a School code: 0779.
650 4 $a Biomedical engineering. $3 535387
650 4 $a Cellular biology. $3 3172791
653 $a Vascular smooth muscle cells
653 $a Adipose derived stromal cells
653 $a Proinflammatory cytokines
690 $a 0541
690 $a 0379
710 2 $a University of Toronto (Canada). $b Biomedical Engineering. $3 2101094
773 0 $t Dissertations Abstracts International $g 81-05B.
790 $a 0779
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=22587504