東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Early Detection of Cancer Metastasis...

Spicer, Graham L. C.

Linked to FindBook

Google Book

Amazon

博客來

Early Detection of Cancer Metastasis at a Synthetic Pre-Metastatic Niche Using Inverse Spectroscopic Optical Coherence Tomography.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Early Detection of Cancer Metastasis at a Synthetic Pre-Metastatic Niche Using Inverse Spectroscopic Optical Coherence Tomography./
Author:	Spicer, Graham L. C.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	220 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
Contained By:	Dissertations Abstracts International81-03B.
Subject:	Medical imaging. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13881616
ISBN:	9781085635912

Early Detection of Cancer Metastasis at a Synthetic Pre-Metastatic Niche Using Inverse Spectroscopic Optical Coherence Tomography.
Spicer, Graham L. C.

Early Detection of Cancer Metastasis at a Synthetic Pre-Metastatic Niche Using Inverse Spectroscopic Optical Coherence Tomography. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 220 p.

Source: Dissertations Abstracts International, Volume: 81-03, Section: B.

Thesis (Ph.D.)--Northwestern University, 2019.

This item must not be sold to any third party vendors.

Cancer progression is a complex process, leading to metastatic spread of primary tumor cells that colonize distant vital organs and mortality if not stopped. Since clinical strategies to stem this progression are still being developed, it is of great importance to detect this end stage metastatic spread as early as possible when the burden of the disease is still manageable by currently available treatments. The pre-metastatic niche describes a local tissue microenvironment where changes in chemokine signaling and immune cell recruitment allow for homing to and metastatic colonization of a specific organ site. An emerging approach to detect metastasis at its earliest stages is through the use of an implanted biomaterial scaffold to mimic this pre-metastatic niche and recruit circulating metastatic cells, lessening metastatic burden in organs and allowing for minimally invasive detection at a subcutaneous location. Using inverse spectroscopic optical coherence tomography (ISOCT), a novel optical spectral tomographic imaging technique, changes in nanoscale structure of the scaffold site in mice have been measured and correlated with presence of metastatic disease. This dissertation describes the development of ISOCT for early detection of metastasis via measurement of the scaffold in vivo and elucidates the milieu of cancer-associated restructuring effects detected with this technique. Laboratory models of carcinogenesis determined ISOCT sensitivity to both extracellular matrix crosslinking and its subsequent effect on proliferative and structural phenotype of cancer cells. A dual-bandwidth ISOCT benchtop instrument and an ISOCT probe housed in a hypodermic needle were developed to address the challenge of imaging and measurement of a scaffold through the skin. Studies of scaffold structure determined increased cancer-associated fibrosis, dysregulated angiogenesis, and differences in scaffold-resident cell population and structure to be the prevailing changes in scaffold structure that allow for potential metastasis screening with ISOCT. Separate development and application of partial wave spectroscopic microscopy for gold nanoparticle imaging, tracking, and spectral analysis allowed for spectral classification of gold nanoparticles based on cellular internalization, with potential for future application to enhancing ISOCT contrast. Development of ISOCT measurement techniques and processing algorithms enabled novel application to imaging and measurement of optical, ultrastructural, and chemical properties in living corals.

ISBN: 9781085635912Subjects--Topical Terms:

3172799
Medical imaging.
Subjects--Index Terms:

Biomaterials

Early Detection of Cancer Metastasis at a Synthetic Pre-Metastatic Niche Using Inverse Spectroscopic Optical Coherence Tomography.
LDR:04014nmm a2200469 4500 001 2272529
005 20201105110116.5
008 220629s2019 ||||||||||||||||| ||eng d
020 $a 9781085635912
035 $a (MiAaPQ)AAI13881616
035 $a AAI13881616
040 $a MiAaPQ $c MiAaPQ
100 1 $a Spicer, Graham L. C. $3 3549964
245 1 0 $a Early Detection of Cancer Metastasis at a Synthetic Pre-Metastatic Niche Using Inverse Spectroscopic Optical Coherence Tomography.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 220 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
500 $a Includes supplementary digital materials.
500 $a Advisor: Backman, Vadim;Shea, Lonnie D.
502 $a Thesis (Ph.D.)--Northwestern University, 2019.
506 $a This item must not be sold to any third party vendors.
520 $a Cancer progression is a complex process, leading to metastatic spread of primary tumor cells that colonize distant vital organs and mortality if not stopped. Since clinical strategies to stem this progression are still being developed, it is of great importance to detect this end stage metastatic spread as early as possible when the burden of the disease is still manageable by currently available treatments. The pre-metastatic niche describes a local tissue microenvironment where changes in chemokine signaling and immune cell recruitment allow for homing to and metastatic colonization of a specific organ site. An emerging approach to detect metastasis at its earliest stages is through the use of an implanted biomaterial scaffold to mimic this pre-metastatic niche and recruit circulating metastatic cells, lessening metastatic burden in organs and allowing for minimally invasive detection at a subcutaneous location. Using inverse spectroscopic optical coherence tomography (ISOCT), a novel optical spectral tomographic imaging technique, changes in nanoscale structure of the scaffold site in mice have been measured and correlated with presence of metastatic disease. This dissertation describes the development of ISOCT for early detection of metastasis via measurement of the scaffold in vivo and elucidates the milieu of cancer-associated restructuring effects detected with this technique. Laboratory models of carcinogenesis determined ISOCT sensitivity to both extracellular matrix crosslinking and its subsequent effect on proliferative and structural phenotype of cancer cells. A dual-bandwidth ISOCT benchtop instrument and an ISOCT probe housed in a hypodermic needle were developed to address the challenge of imaging and measurement of a scaffold through the skin. Studies of scaffold structure determined increased cancer-associated fibrosis, dysregulated angiogenesis, and differences in scaffold-resident cell population and structure to be the prevailing changes in scaffold structure that allow for potential metastasis screening with ISOCT. Separate development and application of partial wave spectroscopic microscopy for gold nanoparticle imaging, tracking, and spectral analysis allowed for spectral classification of gold nanoparticles based on cellular internalization, with potential for future application to enhancing ISOCT contrast. Development of ISOCT measurement techniques and processing algorithms enabled novel application to imaging and measurement of optical, ultrastructural, and chemical properties in living corals.
590 $a School code: 0163.
650 4 $a Medical imaging. $3 3172799
650 4 $a Zoology. $3 518878
650 4 $a Biomedical engineering. $3 535387
650 4 $a Physiology. $3 518431
650 4 $a Epidemiology. $3 568544
650 4 $a Public health. $3 534748
650 4 $a Oncology. $3 751006
650 4 $a Health sciences. $3 3168359
650 4 $a Pathology. $3 643180
653 $a Biomaterials
653 $a Cancer detection
653 $a Coral
653 $a Light scattering
653 $a Optical coherence tomography
653 $a Tissue Engineering
690 $a 0574
690 $a 0472
690 $a 0541
690 $a 0573
690 $a 0571
690 $a 0566
690 $a 0992
690 $a 0766
690 $a 0719
710 2 $a Northwestern University. $b Chemical and Biological Engineering. $3 1023594
773 0 $t Dissertations Abstracts International $g 81-03B.
790 $a 0163
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13881616