東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Plant Uptake and Metabolism of Antim...

Huynh, Khang Vinh.

Linked to FindBook

Google Book

Amazon

博客來

Plant Uptake and Metabolism of Antimicrobials and Antibiotics.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Plant Uptake and Metabolism of Antimicrobials and Antibiotics./
Author:	Huynh, Khang Vinh.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	345 p.
Notes:	Source: Dissertations Abstracts International, Volume: 80-10, Section: B.
Contained By:	Dissertations Abstracts International80-10B.
Subject:	Environmental Studies. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13859536
ISBN:	9781392071489

Plant Uptake and Metabolism of Antimicrobials and Antibiotics.
Huynh, Khang Vinh.

Plant Uptake and Metabolism of Antimicrobials and Antibiotics. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 345 p.

Source: Dissertations Abstracts International, Volume: 80-10, Section: B.

Thesis (Ph.D.)--Michigan State University, 2019.

This item must not be sold to any third party vendors.

Targeted and untargeted metabolomics using high resolution mass spectrometry and multivariate statistical analysis, coupled with 14C-labeled chemicals studies were used to identify novel phytometabolites and quantify the fate of common antimicrobials in plant tissues. Triclocarban (TCC), an antimicrobial that is commonly found in personal care products, was metabolized by jalapeno pepper plants during long-term exposure (12 weeks), leading to the formation of several phase I transformation products (e.g. 2'-OH-TCC and 6-OH-TCC) and phase II glycosylated OH-TCC. Importantly, the concentrations of TCC metabolites were more than 20 times greater than the concentrations of TCC in the above-ground tissues of the pepper plants after 12 weeks. Approximately 95.6% of the TCC was present as metabolites in the fruits. For sulfonamides, upon exposure to the model plant Arabidopsis thaliana, sulfamethazine (SMT) and sulfamethoxazole (SMX) were also prone to extensive metabolism in plant tissues. Untargeted screening of extractable metabolites revealed that glycosylated conjugates were the most abundant metabolites, which accounted for 80-90% of the total metabolites in plant tissues. Other conjugates, such as pterin- and methylsalicyclate-, were present at lower concentrations. Phase I transformation products, such as hydroxyl-, acetyl-, desulfo, and desamino-, were identifed as minor metabolites in plant tissues. For tetracyclines, abiotic transformation and plant metabolism played the key roles in their fate during exposure to Arabidopsis thaliana. Plant metabolism of chlortetracycline (CTC) also led to the formation of glycosylated conjugates and the corresponding 4-epi isomers. More importantly, although CTC was solely added into the experimental reactors, other tetracycline antimicrobials such as tetracycline, 4-epi-tetracycline, demeclocycline, and 4-epi-demeclocycline were detected in the plant tissues. Preliminary studies using soil columns planted with A. thaliana plants showed that phytometabolism of sulfonamides was probably similar with that under hydroponic conditions, with glycosylated conjugates identified as the major phytometabolites. The majority of the uptaken parent sulfonamides and metabolites were found in the plant roots, with limited root-to-shoot translocation. In conclusion, this research indicates that untransformed antimicrobials only represent a small proportion of the total compounds taken up in plant tissues when transformed, conjugated, and sequestered phytometabolites are considered. Consequently, phytometabolism of antimicrobials in planted systems is a critical point for comprehensively addressing human exposure to contaminants of emerging concerns through food chains.

ISBN: 9781392071489Subjects--Topical Terms:

1669635
Environmental Studies.
Subjects--Index Terms:

Antibiotics

Plant Uptake and Metabolism of Antimicrobials and Antibiotics.
LDR:03980nmm a2200397 4500 001 2272465
005 20201105110101.5
008 220629s2019 ||||||||||||||||| ||eng d
020 $a 9781392071489
035 $a (MiAaPQ)AAI13859536
035 $a (MiAaPQ)grad.msu:16675
035 $a AAI13859536
040 $a MiAaPQ $c MiAaPQ
100 1 $a Huynh, Khang Vinh. $3 3549902
245 1 0 $a Plant Uptake and Metabolism of Antimicrobials and Antibiotics.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 345 p.
500 $a Source: Dissertations Abstracts International, Volume: 80-10, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Reinhold, Dawn M.
502 $a Thesis (Ph.D.)--Michigan State University, 2019.
506 $a This item must not be sold to any third party vendors.
520 $a Targeted and untargeted metabolomics using high resolution mass spectrometry and multivariate statistical analysis, coupled with 14C-labeled chemicals studies were used to identify novel phytometabolites and quantify the fate of common antimicrobials in plant tissues. Triclocarban (TCC), an antimicrobial that is commonly found in personal care products, was metabolized by jalapeno pepper plants during long-term exposure (12 weeks), leading to the formation of several phase I transformation products (e.g. 2'-OH-TCC and 6-OH-TCC) and phase II glycosylated OH-TCC. Importantly, the concentrations of TCC metabolites were more than 20 times greater than the concentrations of TCC in the above-ground tissues of the pepper plants after 12 weeks. Approximately 95.6% of the TCC was present as metabolites in the fruits. For sulfonamides, upon exposure to the model plant Arabidopsis thaliana, sulfamethazine (SMT) and sulfamethoxazole (SMX) were also prone to extensive metabolism in plant tissues. Untargeted screening of extractable metabolites revealed that glycosylated conjugates were the most abundant metabolites, which accounted for 80-90% of the total metabolites in plant tissues. Other conjugates, such as pterin- and methylsalicyclate-, were present at lower concentrations. Phase I transformation products, such as hydroxyl-, acetyl-, desulfo, and desamino-, were identifed as minor metabolites in plant tissues. For tetracyclines, abiotic transformation and plant metabolism played the key roles in their fate during exposure to Arabidopsis thaliana. Plant metabolism of chlortetracycline (CTC) also led to the formation of glycosylated conjugates and the corresponding 4-epi isomers. More importantly, although CTC was solely added into the experimental reactors, other tetracycline antimicrobials such as tetracycline, 4-epi-tetracycline, demeclocycline, and 4-epi-demeclocycline were detected in the plant tissues. Preliminary studies using soil columns planted with A. thaliana plants showed that phytometabolism of sulfonamides was probably similar with that under hydroponic conditions, with glycosylated conjugates identified as the major phytometabolites. The majority of the uptaken parent sulfonamides and metabolites were found in the plant roots, with limited root-to-shoot translocation. In conclusion, this research indicates that untransformed antimicrobials only represent a small proportion of the total compounds taken up in plant tissues when transformed, conjugated, and sequestered phytometabolites are considered. Consequently, phytometabolism of antimicrobials in planted systems is a critical point for comprehensively addressing human exposure to contaminants of emerging concerns through food chains.
590 $a School code: 0128.
650 4 $a Environmental Studies. $3 1669635
650 4 $a Plant sciences. $3 3173832
653 $a Antibiotics
653 $a PPCPs
653 $a Phytometabolism
653 $a Plant uptake
653 $a UPLC-QTOF-MS
653 $a Untargeted metabolomics
690 $a 0477
690 $a 0479
710 2 $a Michigan State University. $b Biosystems Engineering. $3 1676179
773 0 $t Dissertations Abstracts International $g 80-10B.
790 $a 0128
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13859536