東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Kinetic, Metabolic and Macromolecula...

Faghihzadeh, Fatemeh.

Linked to FindBook

Google Book

Amazon

博客來

Kinetic, Metabolic and Macromolecular Response of Bacteria to Antimicrobial Agents.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Kinetic, Metabolic and Macromolecular Response of Bacteria to Antimicrobial Agents./
Author:	Faghihzadeh, Fatemeh.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	174 p.
Notes:	Source: Dissertations Abstracts International, Volume: 79-10, Section: B.
Contained By:	Dissertations Abstracts International79-10B.
Subject:	Environmental Health. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10786097
ISBN:	9780355830002

Kinetic, Metabolic and Macromolecular Response of Bacteria to Antimicrobial Agents.
Faghihzadeh, Fatemeh.

Kinetic, Metabolic and Macromolecular Response of Bacteria to Antimicrobial Agents. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 174 p.

Source: Dissertations Abstracts International, Volume: 79-10, Section: B.

Thesis (Ph.D.)--University of Rhode Island, 2018.

This item is not available from ProQuest Dissertations & Theses.

Bacteria have the extraordinary capability to modify their phenotype in response to stress agents. Response mechanisms to stressor (such as nanoparticles or light treatments) are the result of an evolutionary process in which bacteria can become resistant, or adapt to the stressor. Therefore, it is essential to elucidate the bacterial adaptation mechanisms to metal nanoparticles or pulse ultraviolet light since these processes not only can compromise the efficacy of these treatment methods but also has implications to public health issues. Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials in consumer products and medical applications due to their antimicrobial properties. Also, pulsed lights (PL) applications for water treatment purposes are gaining increasing attention. PL has showed higher inactivation of microorganisms and degradation of PAHs, because of its rich and broad-spectrum UV content, high energy peaks and predictable treatment outcomes. However, the fate of microorganisms after exposure to AgNPs and pulsed lights as well as their negative impacts possible on the environment and public health are growing concerns. There are knowledge gaps related to: the studying molecular level change of microorganism after exposure to AgNPs by Fourier-transform infrared spectroscopy (FTIR); Bacterial adaptation to chronic exposure to nanoparticles in continuous culture: kinetic and macromolecular response; and the effect of pulsed ultraviolet light system for removal of polycyclic aromatic hydrocarbon and disinfection of pathogens in drinking water. In this study, Escherichia coli K-12 MG1655 ( E. coli) responses to AgNPs was assessed under batch and continuous conditions. Further, we investigated the response of the same bacteria to PL. In detail, we evaluated antimicrobial agent's impacts on metabolic functions and cell structure such as, colony formation units, membrane permeation, respiration, growth, gene regulation and changes in cellular composition. The results of batch culture for AgNPs toxicity test showed that bacteria developed resistance toward AgNPs and resulted in changes in the genotype and expression in the phenotype. Moreover, in continuous culture, results showed that culture growth conditions significantly affect bacterial response to nanoparticle exposure. Finally, from PL exposure to bacteria we obtained that the antimicrobial efficiency of PL depends on the PL lamp cut-off. This study provides data that have a predominant role to determine the performance of toxicological tests. Hence, the knowledge of nanoparticles fate in different growth condition minimizes the upcoming environmental and public health issues due to releasing nanoparticles release on the ecosystems. Also, understanding the bacterial responses to antimicrobial agents help us to select the more sensible agents for antimicrobial purposes.

ISBN: 9780355830002Subjects--Topical Terms:

578282
Environmental Health.
Subjects--Index Terms:

Cellular components

Kinetic, Metabolic and Macromolecular Response of Bacteria to Antimicrobial Agents.
LDR:04182nmm a2200373 4500 001 2272190
005 20201105105955.5
008 220629s2018 ||||||||||||||||| ||eng d
020 $a 9780355830002
035 $a (MiAaPQ)AAI10786097
035 $a (MiAaPQ)uri:11919
035 $a AAI10786097
040 $a MiAaPQ $c MiAaPQ
100 1 $a Faghihzadeh, Fatemeh. $3 3549620
245 1 0 $a Kinetic, Metabolic and Macromolecular Response of Bacteria to Antimicrobial Agents.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2018
300 $a 174 p.
500 $a Source: Dissertations Abstracts International, Volume: 79-10, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Craver, Vinka O.
502 $a Thesis (Ph.D.)--University of Rhode Island, 2018.
506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be sold to any third party vendors.
520 $a Bacteria have the extraordinary capability to modify their phenotype in response to stress agents. Response mechanisms to stressor (such as nanoparticles or light treatments) are the result of an evolutionary process in which bacteria can become resistant, or adapt to the stressor. Therefore, it is essential to elucidate the bacterial adaptation mechanisms to metal nanoparticles or pulse ultraviolet light since these processes not only can compromise the efficacy of these treatment methods but also has implications to public health issues. Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials in consumer products and medical applications due to their antimicrobial properties. Also, pulsed lights (PL) applications for water treatment purposes are gaining increasing attention. PL has showed higher inactivation of microorganisms and degradation of PAHs, because of its rich and broad-spectrum UV content, high energy peaks and predictable treatment outcomes. However, the fate of microorganisms after exposure to AgNPs and pulsed lights as well as their negative impacts possible on the environment and public health are growing concerns. There are knowledge gaps related to: the studying molecular level change of microorganism after exposure to AgNPs by Fourier-transform infrared spectroscopy (FTIR); Bacterial adaptation to chronic exposure to nanoparticles in continuous culture: kinetic and macromolecular response; and the effect of pulsed ultraviolet light system for removal of polycyclic aromatic hydrocarbon and disinfection of pathogens in drinking water. In this study, Escherichia coli K-12 MG1655 ( E. coli) responses to AgNPs was assessed under batch and continuous conditions. Further, we investigated the response of the same bacteria to PL. In detail, we evaluated antimicrobial agent's impacts on metabolic functions and cell structure such as, colony formation units, membrane permeation, respiration, growth, gene regulation and changes in cellular composition. The results of batch culture for AgNPs toxicity test showed that bacteria developed resistance toward AgNPs and resulted in changes in the genotype and expression in the phenotype. Moreover, in continuous culture, results showed that culture growth conditions significantly affect bacterial response to nanoparticle exposure. Finally, from PL exposure to bacteria we obtained that the antimicrobial efficiency of PL depends on the PL lamp cut-off. This study provides data that have a predominant role to determine the performance of toxicological tests. Hence, the knowledge of nanoparticles fate in different growth condition minimizes the upcoming environmental and public health issues due to releasing nanoparticles release on the ecosystems. Also, understanding the bacterial responses to antimicrobial agents help us to select the more sensible agents for antimicrobial purposes.
590 $a School code: 0186.
650 4 $a Environmental Health. $3 578282
653 $a Cellular components
653 $a Escherichia coli
653 $a Nanoparticles
653 $a Pulsed light
690 $a 0470
710 2 $a University of Rhode Island. $b Civil and Environmental Engineering. $3 2094053
773 0 $t Dissertations Abstracts International $g 79-10B.
790 $a 0186
791 $a Ph.D.
792 $a 2018
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10786097