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Comparative Transcriptomics in the P...

Frangione, Erika .

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Comparative Transcriptomics in the Parasitic Plant Genus Cuscuta Reveals the Effects of Ploidy Level on Gene Expression.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Comparative Transcriptomics in the Parasitic Plant Genus Cuscuta Reveals the Effects of Ploidy Level on Gene Expression./
Author:	Frangione, Erika .
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	58 p.
Notes:	Source: Masters Abstracts International, Volume: 82-01.
Contained By:	Masters Abstracts International82-01.
Subject:	Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27672496
ISBN:	9798662390973

Comparative Transcriptomics in the Parasitic Plant Genus Cuscuta Reveals the Effects of Ploidy Level on Gene Expression.
Frangione, Erika .

Comparative Transcriptomics in the Parasitic Plant Genus Cuscuta Reveals the Effects of Ploidy Level on Gene Expression. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 58 p.

Source: Masters Abstracts International, Volume: 82-01.

Thesis (M.Sc.)--University of Toronto (Canada), 2020.

This item must not be sold to any third party vendors.

Polyploidy is known to influence the rapid evolution observed in angiosperms because it can spark near instantaneous divergence among species. My study system, Cuscuta section Denticulatae, involves two diploid progenitor species, C. denticulata and C. nevadensis, and two relatively recently formed allotetraploids, C. veatchii and C. psorothamnensis. Both parental species are further distinguished into genotypic forms labeled as D1, D2 and N1, N2, respectively. In C. denticulata, autotetraploid populations from D1 have been previously identified. Transcriptomes of both parents were assembled and annotated. To investigate the effects of gene dosage in this system, the transcriptomes were analyzed for differentially expressed genes within each parental species, including between diploid and autotetraploid populations. Differential gene expression was observed between forms D1 and D2 in C. denticulata, with a bias in gene upregulation in D1. Also, a comparative orthologue analysis found a high degree of similarity between the parents and their forms.

ISBN: 9798662390973Subjects--Topical Terms:

530508
Genetics.
Subjects--Index Terms:

Cuscuta

Comparative Transcriptomics in the Parasitic Plant Genus Cuscuta Reveals the Effects of Ploidy Level on Gene Expression.
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245 1 0 $a Comparative Transcriptomics in the Parasitic Plant Genus Cuscuta Reveals the Effects of Ploidy Level on Gene Expression.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 58 p.
500 $a Source: Masters Abstracts International, Volume: 82-01.
500 $a Includes supplementary digital materials.
500 $a Advisor: Stefanovic, Sasa.
502 $a Thesis (M.Sc.)--University of Toronto (Canada), 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Polyploidy is known to influence the rapid evolution observed in angiosperms because it can spark near instantaneous divergence among species. My study system, Cuscuta section Denticulatae, involves two diploid progenitor species, C. denticulata and C. nevadensis, and two relatively recently formed allotetraploids, C. veatchii and C. psorothamnensis. Both parental species are further distinguished into genotypic forms labeled as D1, D2 and N1, N2, respectively. In C. denticulata, autotetraploid populations from D1 have been previously identified. Transcriptomes of both parents were assembled and annotated. To investigate the effects of gene dosage in this system, the transcriptomes were analyzed for differentially expressed genes within each parental species, including between diploid and autotetraploid populations. Differential gene expression was observed between forms D1 and D2 in C. denticulata, with a bias in gene upregulation in D1. Also, a comparative orthologue analysis found a high degree of similarity between the parents and their forms.
590 $a School code: 0779.
650 4 $a Genetics. $3 530508
650 4 $a Bioinformatics. $3 553671
650 4 $a Evolution & development. $3 3172418
653 $a Cuscuta
653 $a Hybridization
653 $a Parasitic plants
653 $a Polyploidy
653 $a RNA-seq
653 $a Transcriptome
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690 $a 0412
710 2 $a University of Toronto (Canada). $b Ecology and Evolutionary Biology. $3 3190014
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