東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Gene Duplication, Divergence, and Wh...

Morgenthaler, Andrew Bank.

Linked to FindBook

Google Book

Amazon

博客來

Gene Duplication, Divergence, and What Lies in Between.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Gene Duplication, Divergence, and What Lies in Between./
Author:	Morgenthaler, Andrew Bank.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	223 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-12.
Contained By:	Dissertations Abstracts International81-12.
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27834114
ISBN:	9798645452117

Gene Duplication, Divergence, and What Lies in Between.
Morgenthaler, Andrew Bank.

Gene Duplication, Divergence, and What Lies in Between. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 223 p.

Source: Dissertations Abstracts International, Volume: 81-12.

Thesis (Ph.D.)--University of Colorado at Boulder, 2020.

This item must not be sold to any third party vendors.

Life has repeatedly evolved new functions throughout its history, allowing life to survive and diversify for 3.8 billion years. The evolution of a novel function frequently requires the evolution of a new gene that encodes for an enzyme with a new function. New enzymes often evolve by amplification and divergence of preexisting genes encoding enzymes with a weak ability to provide a new function. Previous experimental studies have followed the evolutionary trajectory of an amplified gene, but have not considered mutations elsewhere in the genome when fitness is limited by an evolving gene. I have evolved a strain of Escherichia coli in which a secondary promiscuous activity has been recruited to serve an essential function. The gene encoding the 'weak-link' enzyme amplified in all eight populations, but mutations improving the newly needed activity occurred in only one. Most adaptive mutations occurred elsewhere in the genome to improve flux through the bottlenecked pathway. Finally, I compared the evolution of a strain of Escherichia coli grown in two different selective conditions: one in which the original function of an evolving enzyme is no longer required for growth and one in which both the new and original functions are required for growth. While the amplified region of the genome surrounding the evolving gene differed between the two selective conditions, most of the adaptive mutations in the two conditions were similar, suggesting the new activity of the evolving enzyme is more rate-limiting for growth than its native activity. Together these results depict how an organism evolves during the evolution of a new enzymatic function.

ISBN: 9798645452117Subjects--Topical Terms:

517296
Molecular biology.
Subjects--Index Terms:

Enzyme evolution

Gene Duplication, Divergence, and What Lies in Between.
LDR:02856nmm a2200373 4500 001 2270233
005 20200921070800.5
008 220629s2020 ||||||||||||||||| ||eng d
020 $a 9798645452117
035 $a (MiAaPQ)AAI27834114
035 $a AAI27834114
040 $a MiAaPQ $c MiAaPQ
100 1 $a Morgenthaler, Andrew Bank. $3 3547604
245 1 0 $a Gene Duplication, Divergence, and What Lies in Between.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 223 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-12.
500 $a Advisor: Copley, Shelley D.
502 $a Thesis (Ph.D.)--University of Colorado at Boulder, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Life has repeatedly evolved new functions throughout its history, allowing life to survive and diversify for 3.8 billion years. The evolution of a novel function frequently requires the evolution of a new gene that encodes for an enzyme with a new function. New enzymes often evolve by amplification and divergence of preexisting genes encoding enzymes with a weak ability to provide a new function. Previous experimental studies have followed the evolutionary trajectory of an amplified gene, but have not considered mutations elsewhere in the genome when fitness is limited by an evolving gene. I have evolved a strain of Escherichia coli in which a secondary promiscuous activity has been recruited to serve an essential function. The gene encoding the 'weak-link' enzyme amplified in all eight populations, but mutations improving the newly needed activity occurred in only one. Most adaptive mutations occurred elsewhere in the genome to improve flux through the bottlenecked pathway. Finally, I compared the evolution of a strain of Escherichia coli grown in two different selective conditions: one in which the original function of an evolving enzyme is no longer required for growth and one in which both the new and original functions are required for growth. While the amplified region of the genome surrounding the evolving gene differed between the two selective conditions, most of the adaptive mutations in the two conditions were similar, suggesting the new activity of the evolving enzyme is more rate-limiting for growth than its native activity. Together these results depict how an organism evolves during the evolution of a new enzymatic function.
590 $a School code: 0051.
650 4 $a Molecular biology. $3 517296
650 4 $a Evolution & development. $3 3172418
650 4 $a Biochemistry. $3 518028
653 $a Enzyme evolution
653 $a Enzyme promiscuity
653 $a Gene duplication and divergence
653 $a Innovation-amplification-divergence
653 $a Metabolic pathway evolution
690 $a 0307
690 $a 0412
690 $a 0487
710 2 $a University of Colorado at Boulder. $b Molecular, Cellular and Developmental Biology. $3 1293773
773 0 $t Dissertations Abstracts International $g 81-12.
790 $a 0051
791 $a Ph.D.
792 $a 2020
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27834114