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Understanding Freshwater Mussel Adhe...

Li, Kate Xiao Tong.

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Understanding Freshwater Mussel Adhesion Through Recombinant Synthesis of Dresseina bugensis Foot Protein 7.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Understanding Freshwater Mussel Adhesion Through Recombinant Synthesis of Dresseina bugensis Foot Protein 7./
作者:	Li, Kate Xiao Tong.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	99 p.
附註:	Source: Masters Abstracts International, Volume: 81-06.
Contained By:	Masters Abstracts International81-06.
標題:	Biomedical engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=22622639
ISBN:	9781392345016

Understanding Freshwater Mussel Adhesion Through Recombinant Synthesis of Dresseina bugensis Foot Protein 7.
Li, Kate Xiao Tong.

Understanding Freshwater Mussel Adhesion Through Recombinant Synthesis of Dresseina bugensis Foot Protein 7. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 99 p.

Source: Masters Abstracts International, Volume: 81-06.

Thesis (M.A.S.)--University of Toronto (Canada), 2019.

This item must not be sold to any third party vendors.

Zebra and quagga mussels (Dreissena polymorpha and Dreissena bugensis) are the most well-known invasive species in the freshwater ecosystems of North America. Both species exhibit incredible underwater adhesion but little is known regarding the adhesion proteins and mechanisms. In this thesis, the adhesive protein variants in quagga mussel, Dreissena bugensis foot protein 7 (Dbfp7) was selected based on previous MALDI and quantitative LC-MS/MS evidence. This analysis revealed a possible cleavage event undergone by all Dbfp7 variants prior to secretion onto the adhesive interface. For the first time, Dbfp7 variant 9 and variant 14 were produced recombinantly by insertion of quagga mussel Dbfp7 cDNA into an expression vector in E. coli. Dbfp7 variant 9 and 14 were expressed and purified by immobilized metal affinity chromatography at 0.3 mg/mL bacterial culture as a step towards investigating the adhesive properties of Dbfp7 for the development of novel bioinspired adhesives.

ISBN: 9781392345016Subjects--Topical Terms:

535387
Biomedical engineering.
Subjects--Index Terms:

bioadhesive

Understanding Freshwater Mussel Adhesion Through Recombinant Synthesis of Dresseina bugensis Foot Protein 7.
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