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Detection and Serotyping of Foot-and...

Ortega Polo, Rodrigo.

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Detection and Serotyping of Foot-and-Mouth Disease Virus with Laboratory and In Silico Methods.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Detection and Serotyping of Foot-and-Mouth Disease Virus with Laboratory and In Silico Methods./
Author:	Ortega Polo, Rodrigo.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	148 p.
Notes:	Source: Masters Abstracts International, Volume: 80-08.
Contained By:	Masters Abstracts International80-08.
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10981111
ISBN:	9780438796201

Detection and Serotyping of Foot-and-Mouth Disease Virus with Laboratory and In Silico Methods.
Ortega Polo, Rodrigo.

Detection and Serotyping of Foot-and-Mouth Disease Virus with Laboratory and In Silico Methods. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 148 p.

Source: Masters Abstracts International, Volume: 80-08.

Thesis (M.Sc.)--University of Lethbridge (Canada), 2018.

This item must not be sold to any third party vendors.

Foot-and-mouth disease virus (FMDV) is a highly contagious animal pathogen and it has a variable genome and high antigenic variation. There are seven known serotypes of this virus: A, O, C, Asia1, SAT1, SAT2, and SAT3. The rapid detection and serotype characterization of the virus is instrumental for the prompt response by animal health authorities. This thesis presents the design and development of the first electronic microarray assay for the simultaneous detection and subtyping of FMDV. The assay was evaluated in silico and it was tested with 19 synthetic DNA constructs representing all 7 serotypes, followed by the testing with 23 viral RNA samples representing all 7 serotypes. Also, various in silico methods were compared for the classification of FMDV sequences using complete genomes and next generation sequencing (NGS) data. Finally, highly specific and highly sensitive single nucleotide variant signatures that distinguish the seven FMDV serotypes were discovered.

ISBN: 9780438796201Subjects--Topical Terms:

517296
Molecular biology.

Detection and Serotyping of Foot-and-Mouth Disease Virus with Laboratory and In Silico Methods.
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500 $a Source: Masters Abstracts International, Volume: 80-08.
500 $a Publisher info.: Dissertation/Thesis.
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506 $a This item must not be sold to any third party vendors.
520 $a Foot-and-mouth disease virus (FMDV) is a highly contagious animal pathogen and it has a variable genome and high antigenic variation. There are seven known serotypes of this virus: A, O, C, Asia1, SAT1, SAT2, and SAT3. The rapid detection and serotype characterization of the virus is instrumental for the prompt response by animal health authorities. This thesis presents the design and development of the first electronic microarray assay for the simultaneous detection and subtyping of FMDV. The assay was evaluated in silico and it was tested with 19 synthetic DNA constructs representing all 7 serotypes, followed by the testing with 23 viral RNA samples representing all 7 serotypes. Also, various in silico methods were compared for the classification of FMDV sequences using complete genomes and next generation sequencing (NGS) data. Finally, highly specific and highly sensitive single nucleotide variant signatures that distinguish the seven FMDV serotypes were discovered.
590 $a School code: 1112.
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