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Local Nucleic Acid Base Conformation...

Ji, Huiying.

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Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA./
Author:	Ji, Huiying.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	180 p.
Notes:	Source: Dissertation Abstracts International, Volume: 80-08(E), Section: B.
Contained By:	Dissertation Abstracts International80-08B(E).
Subject:	Chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13425709
ISBN:	9781392059418

Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
Ji, Huiying.

Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 180 p.

Source: Dissertation Abstracts International, Volume: 80-08(E), Section: B.

Thesis (Ph.D.)--University of Oregon, 2019.

Understanding the local conformations of DNA at the level of individual nucleic acid bases is important for the study of the mechanism of DNA sequence-dependent behavior. Here we apply linear absorption, circular dichroism (CD), and fluorescence spectroscopy to study the DNA local base conformation using 6-methyl Isoxanthopterin (6-MI) labeled DNA. We interpret excitation--emission peak shift (EES) measurements of the 6-MI, both as a ribonucleotide monophosphate in solution and as a site-specific substituent for guanine in various DNA constructs, by implementing a simple two-state model. We show that the spectroscopic properties of the 6-MI probe in DNA can be used to obtain detailed information about local base conformations and conformational heterogeneity and fluctuations. Based on these findings, we apply a simple theoretical model to calculate CD of 6-MI substituted DNA constructs. We find that our model can be used to extract basesequence- dependent information about the local conformation of the 6-MI probe as modulated by the local base or base-pair environment. We next apply 6-MI to probe the ligand insertion of small molecules to duplex DNA, further extending the potential of 6- MI as a useful reporter of local nucleic acid base conformation. These studies served to establish a new level of sophistication in qualitatively analyzing 6-MI structural behavior in terms of local base stacking and unstacking conformations.

ISBN: 9781392059418Subjects--Topical Terms:

516420
Chemistry.

Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
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245 1 0 $a Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
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300 $a 180 p.
500 $a Source: Dissertation Abstracts International, Volume: 80-08(E), Section: B.
500 $a Advisers: Andrew H. Marcud; Peter H. von Hippel.
502 $a Thesis (Ph.D.)--University of Oregon, 2019.
520 $a Understanding the local conformations of DNA at the level of individual nucleic acid bases is important for the study of the mechanism of DNA sequence-dependent behavior. Here we apply linear absorption, circular dichroism (CD), and fluorescence spectroscopy to study the DNA local base conformation using 6-methyl Isoxanthopterin (6-MI) labeled DNA. We interpret excitation--emission peak shift (EES) measurements of the 6-MI, both as a ribonucleotide monophosphate in solution and as a site-specific substituent for guanine in various DNA constructs, by implementing a simple two-state model. We show that the spectroscopic properties of the 6-MI probe in DNA can be used to obtain detailed information about local base conformations and conformational heterogeneity and fluctuations. Based on these findings, we apply a simple theoretical model to calculate CD of 6-MI substituted DNA constructs. We find that our model can be used to extract basesequence- dependent information about the local conformation of the 6-MI probe as modulated by the local base or base-pair environment. We next apply 6-MI to probe the ligand insertion of small molecules to duplex DNA, further extending the potential of 6- MI as a useful reporter of local nucleic acid base conformation. These studies served to establish a new level of sophistication in qualitatively analyzing 6-MI structural behavior in terms of local base stacking and unstacking conformations.
520 $a This dissertation contains previously published and unpublished co-authored material.
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