東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Identification and Characterization ...

Alkahtani, Jawaher Salman.

Linked to FindBook

Google Book

Amazon

博客來

Identification and Characterization of Salinity Tolerance Genes by Activation Tagging in Arabidopsis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Identification and Characterization of Salinity Tolerance Genes by Activation Tagging in Arabidopsis./
Author:	Alkahtani, Jawaher Salman.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	188 p.
Notes:	Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Contained By:	Dissertation Abstracts International79-10B(E).
Subject:	Plant sciences. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10810519
ISBN:	9780438007284

Identification and Characterization of Salinity Tolerance Genes by Activation Tagging in Arabidopsis.
Alkahtani, Jawaher Salman.

Identification and Characterization of Salinity Tolerance Genes by Activation Tagging in Arabidopsis. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 188 p.

Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.

Thesis (Ph.D.)--University of Arkansas, 2018.

Salinity often affects irrigated areas in arid and semi-arid regions of the world. The existence and accumulation of soluble salts in the soil layers limit the growth of crops essential for our food. Salt stress dramatically affects plant growth, plant development, as well as crop yield. Arabidopsis thaliana is the plant model that provides a comprehensive knowledge of plant development, genetics and physiology, and response to abiotic stresses such as salinity. The redundancy of genes due to duplication, even in the simple model genome of Arabidopsis, limits the value of knockout (KO) mutagenesis to provide complete information on gene function. 'Gain-of-function' mutants are an alternative genetic tool to identify gene functions for redundant genes, and those with small effect or that respond to an environmental condition. Transposon-mediated 'activation tagging' is an efficient genetic tool that can randomly generate 'gain-of-function' mutants for a large number of genes. In the method used here, the transposable element Enhancer-Inhibitor (En-I/dSpm) system of maize was modified to develop an activation tag (AT) mutant library in Arabidopsis. The mobile I-AT transposon contains a transcriptional enhancer, from the cauliflower mosaic virus (CaMV) 35S promoter, located close to the right border of the transposon. This I-AT element was mobilized to randomly insert into the plant genome by transposition from the T-DNA, and can give rise to mutants differing in the level of overexpression of the adjacent genes. Consequently, the gain-of-function dominant phenotypes generated are displayed by the I-AT plants due to enhanced expression of the gene(s) adjacent to the 35S enhancer. In this study, the I-AT library was used to screen for salt tolerance, identified by enhanced growth or biomass of the tagged mutants compared to the wild-type grown in saline conditions. A number of tagged salt tolerance candidate genes were identified flanking the I-AT insertion, and their tagged genes characterized for their role in salt tolerance.

ISBN: 9780438007284Subjects--Topical Terms:

3173832
Plant sciences.

Identification and Characterization of Salinity Tolerance Genes by Activation Tagging in Arabidopsis.
LDR:03005nmm a2200289 4500 001 2203029
005 20190528122951.5
008 201008s2018 ||||||||||||||||| ||eng d
020 $a 9780438007284
035 $a (MiAaPQ)AAI10810519
035 $a (MiAaPQ)uark:12942
035 $a AAI10810519
040 $a MiAaPQ $c MiAaPQ
100 1 $a Alkahtani, Jawaher Salman. $3 3429809
245 1 0 $a Identification and Characterization of Salinity Tolerance Genes by Activation Tagging in Arabidopsis.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2018
300 $a 188 p.
500 $a Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
500 $a Adviser: Andy Pereira.
502 $a Thesis (Ph.D.)--University of Arkansas, 2018.
520 $a Salinity often affects irrigated areas in arid and semi-arid regions of the world. The existence and accumulation of soluble salts in the soil layers limit the growth of crops essential for our food. Salt stress dramatically affects plant growth, plant development, as well as crop yield. Arabidopsis thaliana is the plant model that provides a comprehensive knowledge of plant development, genetics and physiology, and response to abiotic stresses such as salinity. The redundancy of genes due to duplication, even in the simple model genome of Arabidopsis, limits the value of knockout (KO) mutagenesis to provide complete information on gene function. 'Gain-of-function' mutants are an alternative genetic tool to identify gene functions for redundant genes, and those with small effect or that respond to an environmental condition. Transposon-mediated 'activation tagging' is an efficient genetic tool that can randomly generate 'gain-of-function' mutants for a large number of genes. In the method used here, the transposable element Enhancer-Inhibitor (En-I/dSpm) system of maize was modified to develop an activation tag (AT) mutant library in Arabidopsis. The mobile I-AT transposon contains a transcriptional enhancer, from the cauliflower mosaic virus (CaMV) 35S promoter, located close to the right border of the transposon. This I-AT element was mobilized to randomly insert into the plant genome by transposition from the T-DNA, and can give rise to mutants differing in the level of overexpression of the adjacent genes. Consequently, the gain-of-function dominant phenotypes generated are displayed by the I-AT plants due to enhanced expression of the gene(s) adjacent to the 35S enhancer. In this study, the I-AT library was used to screen for salt tolerance, identified by enhanced growth or biomass of the tagged mutants compared to the wild-type grown in saline conditions. A number of tagged salt tolerance candidate genes were identified flanking the I-AT insertion, and their tagged genes characterized for their role in salt tolerance.
590 $a School code: 0011.
650 4 $a Plant sciences. $3 3173832
690 $a 0479
710 2 $a University of Arkansas. $b Cell and Molecular Biology. $3 3169436
773 0 $t Dissertation Abstracts International $g 79-10B(E).
790 $a 0011
791 $a Ph.D.
792 $a 2018
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10810519