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Point Spread Function Engineered Sup...

Schroeder, Bryce.

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Point Spread Function Engineered Super-Resolution Microscopy.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Point Spread Function Engineered Super-Resolution Microscopy./
Author:	Schroeder, Bryce.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	49 p.
Notes:	Source: Dissertation Abstracts International, Volume: 80-02(E), Section: B.
Contained By:	Dissertation Abstracts International80-02B(E).
Subject:	Optics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10790502
ISBN:	9780438561960

Point Spread Function Engineered Super-Resolution Microscopy.
Schroeder, Bryce.

Point Spread Function Engineered Super-Resolution Microscopy. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 49 p.

Source: Dissertation Abstracts International, Volume: 80-02(E), Section: B.

Thesis (Ph.D.)--State University of New York at Stony Brook, 2018.

The application of point-spread function engineering to super-resolution fluorescence microscopy is investigated using a spatial light modulator to apply phase modulation to the emission path of a high-resolution microscopy system. Methods for recovering 3D position information from single fluorescent molecules are explored in the context of STORM (Stochastic Optical Reconstruction Microscopy), as is a technique for obtaining spectral information from the fluorescent emitters. The problem of optimizing the signal-to-noise ratio to overcome photon loss in the modulation system is discussed. Representative applications of the developed techniques are considered. General methods for point-spread function engineering using optimization metaheuristics are developed, including a versatile software framework for the design and evaluation of phase modulation patterns.

ISBN: 9780438561960Subjects--Topical Terms:

517925
Optics.

Point Spread Function Engineered Super-Resolution Microscopy.
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500 $a Source: Dissertation Abstracts International, Volume: 80-02(E), Section: B.
500 $a Adviser: Shu Jia.
502 $a Thesis (Ph.D.)--State University of New York at Stony Brook, 2018.
520 $a The application of point-spread function engineering to super-resolution fluorescence microscopy is investigated using a spatial light modulator to apply phase modulation to the emission path of a high-resolution microscopy system. Methods for recovering 3D position information from single fluorescent molecules are explored in the context of STORM (Stochastic Optical Reconstruction Microscopy), as is a technique for obtaining spectral information from the fluorescent emitters. The problem of optimizing the signal-to-noise ratio to overcome photon loss in the modulation system is discussed. Representative applications of the developed techniques are considered. General methods for point-spread function engineering using optimization metaheuristics are developed, including a versatile software framework for the design and evaluation of phase modulation patterns.
590 $a School code: 0771.
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650 4 $a Biomedical engineering. $3 535387
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