東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Optimization of a Viral System to Pr...

Diamos, Andy.

Linked to FindBook

Google Book

Amazon

博客來

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants./
Author:	Diamos, Andy.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	272 p.
Notes:	Source: Dissertation Abstracts International, Volume: 79-04(E), Section: B.
Contained By:	Dissertation Abstracts International79-04B(E).
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10681475
ISBN:	9780355512892

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.
Diamos, Andy.

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 272 p.

Source: Dissertation Abstracts International, Volume: 79-04(E), Section: B.

Thesis (Ph.D.)--Arizona State University, 2017.

Plants are a promising upcoming platform for production of vaccine components and other desirable pharmaceutical proteins that can only, at present, be made in living systems. The unique soil microbe Agrobacterium tumefaciens can transfer DNA to plants very efficiently, essentially turning plants into factories capable of producing virtually any gene. While genetically modified bacteria have historically been used for producing useful biopharmaceuticals like human insulin, plants can assemble much more complicated proteins, like human antibodies, that bacterial systems cannot. As plants do not harbor human pathogens, they are also safer alternatives than animal cell cultures. Additionally, plants can be grown very cheaply, in massive quantities.

ISBN: 9780355512892Subjects--Topical Terms:

517296
Molecular biology.

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.
LDR:03190nmm a2200325 4500 001 2160497
005 20180727091503.5
008 190424s2017 ||||||||||||||||| ||eng d
020 $a 9780355512892
035 $a (MiAaPQ)AAI10681475
035 $a (MiAaPQ)asu:17585
035 $a AAI10681475
040 $a MiAaPQ $c MiAaPQ
100 1 $a Diamos, Andy. $3 3348419
245 1 0 $a Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2017
300 $a 272 p.
500 $a Source: Dissertation Abstracts International, Volume: 79-04(E), Section: B.
500 $a Adviser: Hugh S. Mason.
502 $a Thesis (Ph.D.)--Arizona State University, 2017.
520 $a Plants are a promising upcoming platform for production of vaccine components and other desirable pharmaceutical proteins that can only, at present, be made in living systems. The unique soil microbe Agrobacterium tumefaciens can transfer DNA to plants very efficiently, essentially turning plants into factories capable of producing virtually any gene. While genetically modified bacteria have historically been used for producing useful biopharmaceuticals like human insulin, plants can assemble much more complicated proteins, like human antibodies, that bacterial systems cannot. As plants do not harbor human pathogens, they are also safer alternatives than animal cell cultures. Additionally, plants can be grown very cheaply, in massive quantities.
520 $a In my research, I have studied the genetic mechanisms that underlie gene expression, in order to improve plant-based biopharmaceutical production. To do this, inspiration was drawn from naturally-occurring gene regulatory mechanisms, especially those from plant viruses, which have evolved mechanisms to co-opt the plant cellular machinery to produce high levels of viral proteins. By testing, modifying, and combining genetic elements from diverse sources, an optimized expression system has been developed that allows very rapid production of vaccine components, monoclonal antibodies, and other biopharmaceuticals. To improve target gene expression while maintaining the health and function of the plants, I identified, studied, and modified 5' untranslated regions, combined gene terminators, and a nuclear matrix attachment region. The replication mechanisms of a plant geminivirus were also studied, which lead to additional strategies to produce more toxic biopharmaceutical proteins. Finally, the mechanisms employed by a geminivirus to spread between cells were investigated. It was demonstrated that these movement mechanisms can be functionally transplanted into a separate genus of geminivirus, allowing modified virus-based gene expression vectors to be spread between neighboring plant cells. Additionally, my work helps shed light on the basic genetic mechanisms employed by all living organisms to control gene expression.
590 $a School code: 0010.
650 4 $a Molecular biology. $3 517296
650 4 $a Genetics. $3 530508
650 4 $a Virology. $3 642304
690 $a 0307
690 $a 0369
690 $a 0720
710 2 $a Arizona State University. $b Microbiology. $3 3348420
773 0 $t Dissertation Abstracts International $g 79-04B(E).
790 $a 0010
791 $a Ph.D.
792 $a 2017
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10681475