東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Nitrogen Isotope Fractionation and T...

Armstrong, C. Taylor.

Linked to FindBook

Google Book

Amazon

博客來

Nitrogen Isotope Fractionation and Toxin Production During the Uptake of Micromolar Concentrations of Nitrate, Ammonium, and Urea by a Marine Dinoflagellate.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Nitrogen Isotope Fractionation and Toxin Production During the Uptake of Micromolar Concentrations of Nitrate, Ammonium, and Urea by a Marine Dinoflagellate./
Author:	Armstrong, C. Taylor.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	93 p.
Notes:	Source: Masters Abstracts International, Volume: 56-04.
Contained By:	Masters Abstracts International56-04(E).
Subject:	Aquatic sciences. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10260838
ISBN:	9781369702309

Nitrogen Isotope Fractionation and Toxin Production During the Uptake of Micromolar Concentrations of Nitrate, Ammonium, and Urea by a Marine Dinoflagellate.
Armstrong, C. Taylor.

Nitrogen Isotope Fractionation and Toxin Production During the Uptake of Micromolar Concentrations of Nitrate, Ammonium, and Urea by a Marine Dinoflagellate. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 93 p.

Source: Masters Abstracts International, Volume: 56-04.

Thesis (M.S.)--The College of William and Mary, 2017.

Despite an increased global interest in harmful algal bloom (HAB) species and eutrophication, the relationship between nutrient sources and changes in species composition or toxicity remains unclear. Stable isotopes are routinely used to identify and track nitrogen (N) sources to water bodies, as sources can be differentiated based on stable isotope values. While literature is available describing N fractionation by diatoms and coccolithophores, data are greatly lacking regarding isotope fractionation by dinoflagellates. Here we investigate the fractionation of nitrogen isotopes by saxitoxin-producing Alexandrium fundyense, to validate the use of the delta 15N of particulate organic matter and identify the nitrogen source fueling a dinoflagellate bloom and its toxicity. The effects of N chemical form on isotope fractionation, toxin content, and toxicity, were investigated using isolates in single-N and mixed-N experiments. Growth on NO3 -, NH4+, or urea, resulted in isotope fractionation of 2.761.48&permil;, 29.019.32&permil;, or 0.340.19&permil;, respectively, with the lowest cellular toxicity and toxin quotas reported during urea utilization. Toxin composition and growth rates, however, remained constant across all N treatments, showing no effects of NO3-, NH 4+, or urea utilization. Alexandrium fundyense was then preconditioned to either NO3-, NH4 +, or urea, and abruptly inoculated into mixed-N medium containing all three chemical forms. All treatments initially utilized NH4 + and urea upon inoculation into mixed medium, suggesting no effect of preconditioning. Cells only began utilizing NO3- after NH4+ decreased below 2-4 microM in the medium. During the inhibition of NO3- uptake by NH 4+ utilization, the cellular delta15N was at its lowest (-5&permil;), and through the course of the experiment, the delta15N continuously changed to mimic the isotope value of the most recent N source(s) being utilized. When utilizing multiple sources, the isotope signature of the cells fell between the signal of the two N sources. Together this suggests that in NO3- and urea rich environments, the delta15NPOM would reliably look like the source or sources of nitrogen utilized, but that caution should be taken in NH4+ rich environments where the large value could lead to misinterpretation of the signal. Nutrients are only one factor influencing bloom dynamics, but information about the relative importance of natural or anthropogenic nutrients in the development and toxicity of bloom events is necessary to predict future shifts in phytoplankton species composition, density, and toxicity.

ISBN: 9781369702309Subjects--Topical Terms:

3174300
Aquatic sciences.

Nitrogen Isotope Fractionation and Toxin Production During the Uptake of Micromolar Concentrations of Nitrate, Ammonium, and Urea by a Marine Dinoflagellate.
LDR:03649nmm a2200313 4500 001 2158682
005 20180618102530.5
008 190424s2017 ||||||||||||||||| ||eng d
020 $a 9781369702309
035 $a (MiAaPQ)AAI10260838
035 $a (MiAaPQ)vims:10013
035 $a AAI10260838
040 $a MiAaPQ $c MiAaPQ
100 1 $a Armstrong, C. Taylor. $3 3346511
245 1 0 $a Nitrogen Isotope Fractionation and Toxin Production During the Uptake of Micromolar Concentrations of Nitrate, Ammonium, and Urea by a Marine Dinoflagellate.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2017
300 $a 93 p.
500 $a Source: Masters Abstracts International, Volume: 56-04.
500 $a Adviser: Juliette L. Smith.
502 $a Thesis (M.S.)--The College of William and Mary, 2017.
520 $a Despite an increased global interest in harmful algal bloom (HAB) species and eutrophication, the relationship between nutrient sources and changes in species composition or toxicity remains unclear. Stable isotopes are routinely used to identify and track nitrogen (N) sources to water bodies, as sources can be differentiated based on stable isotope values. While literature is available describing N fractionation by diatoms and coccolithophores, data are greatly lacking regarding isotope fractionation by dinoflagellates. Here we investigate the fractionation of nitrogen isotopes by saxitoxin-producing Alexandrium fundyense, to validate the use of the delta 15N of particulate organic matter and identify the nitrogen source fueling a dinoflagellate bloom and its toxicity. The effects of N chemical form on isotope fractionation, toxin content, and toxicity, were investigated using isolates in single-N and mixed-N experiments. Growth on NO3 -, NH4+, or urea, resulted in isotope fractionation of 2.761.48&permil;, 29.019.32&permil;, or 0.340.19&permil;, respectively, with the lowest cellular toxicity and toxin quotas reported during urea utilization. Toxin composition and growth rates, however, remained constant across all N treatments, showing no effects of NO3-, NH 4+, or urea utilization. Alexandrium fundyense was then preconditioned to either NO3-, NH4 +, or urea, and abruptly inoculated into mixed-N medium containing all three chemical forms. All treatments initially utilized NH4 + and urea upon inoculation into mixed medium, suggesting no effect of preconditioning. Cells only began utilizing NO3- after NH4+ decreased below 2-4 microM in the medium. During the inhibition of NO3- uptake by NH 4+ utilization, the cellular delta15N was at its lowest (-5&permil;), and through the course of the experiment, the delta15N continuously changed to mimic the isotope value of the most recent N source(s) being utilized. When utilizing multiple sources, the isotope signature of the cells fell between the signal of the two N sources. Together this suggests that in NO3- and urea rich environments, the delta15NPOM would reliably look like the source or sources of nitrogen utilized, but that caution should be taken in NH4+ rich environments where the large value could lead to misinterpretation of the signal. Nutrients are only one factor influencing bloom dynamics, but information about the relative importance of natural or anthropogenic nutrients in the development and toxicity of bloom events is necessary to predict future shifts in phytoplankton species composition, density, and toxicity.
590 $a School code: 0261.
650 4 $a Aquatic sciences. $3 3174300
650 4 $a Biology. $3 522710
650 4 $a Toxicology. $3 556884
690 $a 0792
690 $a 0306
690 $a 0383
710 2 $a The College of William and Mary. $b School of Marine Science. $3 3281332
773 0 $t Masters Abstracts International $g 56-04(E).
790 $a 0261
791 $a M.S.
792 $a 2017
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10260838