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Stretchable impedance spectroscopy s...

Zhang, Xudong.

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Stretchable impedance spectroscopy sensor for mammalian cell studies.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Stretchable impedance spectroscopy sensor for mammalian cell studies./
作者:	Zhang, Xudong.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2016,
面頁冊數:	156 p.
附註:	Source: Dissertation Abstracts International, Volume: 78-04(E), Section: B.
Contained By:	Dissertation Abstracts International78-04B(E).
標題:	Mechanical engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10159947
ISBN:	9781369148749

Stretchable impedance spectroscopy sensor for mammalian cell studies.
Zhang, Xudong.

Stretchable impedance spectroscopy sensor for mammalian cell studies. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 156 p.

Source: Dissertation Abstracts International, Volume: 78-04(E), Section: B.

Thesis (Ph.D.)--The City College of New York, 2016.

Endothelial cells (ECs) are exposed to circumferential cyclic stretch that modulates cell functions, morphology, proliferation and gene expression. The research of mechanical stimuli on ECs proliferation is significant to investigate wound healing, thrombus formation and vascular biology. However, the influence of circumference stretch duration and magnitude on ECs remains unclear. To date, fluorescent staining is a common method for analyzing cell status and proliferation. However fluorescent dyes are usually phototoxic and invasive influencing cell viability and can only be carried out at endpoints without dynamic information. Biosensors are able to detect the cell behavior and morphology in real time based on Electric Cell-substrate Impedance Sensing (ECIS) technique with a non-invasive approach in real time.

ISBN: 9781369148749Subjects--Topical Terms:

649730
Mechanical engineering.

Stretchable impedance spectroscopy sensor for mammalian cell studies.
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520 $a Endothelial cells (ECs) are exposed to circumferential cyclic stretch that modulates cell functions, morphology, proliferation and gene expression. The research of mechanical stimuli on ECs proliferation is significant to investigate wound healing, thrombus formation and vascular biology. However, the influence of circumference stretch duration and magnitude on ECs remains unclear. To date, fluorescent staining is a common method for analyzing cell status and proliferation. However fluorescent dyes are usually phototoxic and invasive influencing cell viability and can only be carried out at endpoints without dynamic information. Biosensors are able to detect the cell behavior and morphology in real time based on Electric Cell-substrate Impedance Sensing (ECIS) technique with a non-invasive approach in real time.
520 $a In this study, ECIS sensors with microfluidic cell culture chambers were developed and it can shorten the response time compared with traditional open well ECIS sensor in water toxicity testing.
520 $a An ECIS mathematical model was established to provide a theoretical foundation and optimize the sensor design. The correlation between the sensor detection sensitivity and its design was determined through the experimental data and was supported by the mathematic model. A stretchable impedance spectroscopy biosensor and its fabrication procedures have been developed based on ECIS technique. Through these optimized procedures, Au coated ECIS electrodes were successfully fabricated on the stretchable PDMS membrane. The electrodes and electrical connections on the stretchable ECIS sensor maintained excellent conductivity after cyclic stretch. The stretchable impedance sensors were evaluated by measuring cell impedance with cyclic stretching. The elastic properties of the ECIS sensors enable the simulation and replication of the dynamic environment of organism, in such ways as pulsation, bending and stretching. The stretchable ECIS sensors enable investigation on cell behavior that undergoes mechanical stimuli in biological tissue. Bovine aortic endothelial cells (BAECs) have been studied, because they are in an environment under cyclical stretch. The stretchable impedance sensors were used to analyze the proliferation of BAECs with different cyclic mechanical stimuli in vitro. The results of fluorescent cell proliferation assays confirm that the stretchable ECIS sensors are able to analyze the real-time proliferation of BAECs in a non-invasive and label-free method.
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