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Novel Multi-Axial Cartesian Bioreact...

Lu, Jeffrey Cheng-Tung.

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Novel Multi-Axial Cartesian Bioreactor Engineered for Stimulation of Mechanically Regulated Gene Promoters in Human Chondrocytes.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Novel Multi-Axial Cartesian Bioreactor Engineered for Stimulation of Mechanically Regulated Gene Promoters in Human Chondrocytes./
Author:	Lu, Jeffrey Cheng-Tung.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	82 p.
Notes:	Source: Dissertation Abstracts International, Volume: 78-10(E), Section: B.
Contained By:	Dissertation Abstracts International78-10B(E).
Subject:	Biomedical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10252301
ISBN:	9781369795783

Novel Multi-Axial Cartesian Bioreactor Engineered for Stimulation of Mechanically Regulated Gene Promoters in Human Chondrocytes.
Lu, Jeffrey Cheng-Tung.

Novel Multi-Axial Cartesian Bioreactor Engineered for Stimulation of Mechanically Regulated Gene Promoters in Human Chondrocytes. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 82 p.

Source: Dissertation Abstracts International, Volume: 78-10(E), Section: B.

Thesis (Ph.D.)--University of California, Davis, 2017.

Insufficient knowledge behind chondrocyte mechanotransduction remains a core factor of why osteoarthritis aetiology is poorly understood. A large variation in conditions used in mechanical loading studies have resulted in large variations in the metabolic response of chondrocytes, especially in cell-seeded scaffolds. We seek to elucidate specific chondrocyte mechanotransduction pathways to determine how cartilage tissues are maintained and adapted by focusing on how differing types of mechanical loading influence DNA elements that confer mechanical sensitivity to a gene. Our hypothesis was that specific mechano-regulatory DNA elements exist within the promoter region of mechanoresponsive genes in chondrocytes responsible for cartilage maintenance and repair. A custom multi-axial bioreactor was designed and fabricated to decouple matrix stimulation from fluid shear stresses through uniform mechanical loading. Chondrocytes transduced with mechanoresponsive hCOMP promoter sequences driving luciferase reporters identified new upregulatory and silencing gene sequences through responses to different types of mechanical loading. These results identified important elements in the chondrocyte mechanotransduction pathway, as well as providing insight into mechanical bioreactor design to minimize large variations in metabolic response.

ISBN: 9781369795783Subjects--Topical Terms:

535387
Biomedical engineering.

Novel Multi-Axial Cartesian Bioreactor Engineered for Stimulation of Mechanically Regulated Gene Promoters in Human Chondrocytes.
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500 $a Adviser: Dominik R. Haudenschild.
502 $a Thesis (Ph.D.)--University of California, Davis, 2017.
520 $a Insufficient knowledge behind chondrocyte mechanotransduction remains a core factor of why osteoarthritis aetiology is poorly understood. A large variation in conditions used in mechanical loading studies have resulted in large variations in the metabolic response of chondrocytes, especially in cell-seeded scaffolds. We seek to elucidate specific chondrocyte mechanotransduction pathways to determine how cartilage tissues are maintained and adapted by focusing on how differing types of mechanical loading influence DNA elements that confer mechanical sensitivity to a gene. Our hypothesis was that specific mechano-regulatory DNA elements exist within the promoter region of mechanoresponsive genes in chondrocytes responsible for cartilage maintenance and repair. A custom multi-axial bioreactor was designed and fabricated to decouple matrix stimulation from fluid shear stresses through uniform mechanical loading. Chondrocytes transduced with mechanoresponsive hCOMP promoter sequences driving luciferase reporters identified new upregulatory and silencing gene sequences through responses to different types of mechanical loading. These results identified important elements in the chondrocyte mechanotransduction pathway, as well as providing insight into mechanical bioreactor design to minimize large variations in metabolic response.
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