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Structure and mechanochemistry of ic...

Zeng, Cheng.

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Structure and mechanochemistry of icosahedral viruses and virus shells studied by atomic force microscopy.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Structure and mechanochemistry of icosahedral viruses and virus shells studied by atomic force microscopy./
Author:	Zeng, Cheng.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	158 p.
Notes:	Source: Dissertation Abstracts International, Volume: 78-07(E), Section: B.
Contained By:	Dissertation Abstracts International78-07B(E).
Subject:	Physical chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10257038
ISBN:	9781369593983

Structure and mechanochemistry of icosahedral viruses and virus shells studied by atomic force microscopy.
Zeng, Cheng.

Structure and mechanochemistry of icosahedral viruses and virus shells studied by atomic force microscopy. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 158 p.

Source: Dissertation Abstracts International, Volume: 78-07(E), Section: B.

Thesis (Ph.D.)--Indiana University, 2017.

Viruses are ubiquitous biological entities that have been increasingly adopted for positive applications. Fundamental knowledge of virus structure and stabilities is vital for understanding virus infection and development of novel virus-based materials. During an infectious cycle, the viral capsid has to pass through numerous cellular barriers and simultaneously encounter forces. Thus, it is important to understand how virus capsids react to mechanical perturbations.

ISBN: 9781369593983Subjects--Topical Terms:

1981412
Physical chemistry.

Structure and mechanochemistry of icosahedral viruses and virus shells studied by atomic force microscopy.
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100 1 $a Zeng, Cheng. $3 3341202
245 1 0 $a Structure and mechanochemistry of icosahedral viruses and virus shells studied by atomic force microscopy.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2017
300 $a 158 p.
500 $a Source: Dissertation Abstracts International, Volume: 78-07(E), Section: B.
500 $a Adviser: Bogdan Dragnea.
502 $a Thesis (Ph.D.)--Indiana University, 2017.
520 $a Viruses are ubiquitous biological entities that have been increasingly adopted for positive applications. Fundamental knowledge of virus structure and stabilities is vital for understanding virus infection and development of novel virus-based materials. During an infectious cycle, the viral capsid has to pass through numerous cellular barriers and simultaneously encounter forces. Thus, it is important to understand how virus capsids react to mechanical perturbations.
520 $a This dissertation work takes on the structure and mechanochemistry of icosahedral viruses and virus shells. Atomic force microscopy (AFM) imaging and nano-indentation are the primary methods utilized in this study because they provide good spatial and force resolution for understanding the nano-mechanical events. The types of strain that were examined include compression, flattening, shrinkage, collapse, etc.
520 $a Firstly, we studied the adsorption of viruses on AFM substrates to understand the impact of adhesive interactions. We have discovered an orientation bias and a surface-induced deformation upon adsorption. A physical model was also proposed to describe the mechanochemical properties of the capsid without performing indentation. Secondly, an AFM nano-indentation assay in presence of macromolecules was carried out to mimic crowding effect. A small external osmotic pressure was found to stiffen the capsid, while higher osmotic pressure can soften the capsid. Thirdly, the resilience of virus capsids was studied by dehydration and nano-indentation. We observed a remarkable recovery of capsid after a dehydration/rehydration cycle. Plastic deformation was observed for BMV after deep indentation. Fourthly, the capsomeric structure of virus-like particles (VLPs) was determined for the first time for both spherical and spherocylindrical particles. Structural analyses were performed to understand the origin of defects as well. Lastly, the surface adsorption and elasticity was examined for a gene therapy vector. Connections were found between mechanochemical properties and encapsulated cargo. A statistical model was put forward for prediction of cargo type.
590 $a School code: 0093.
650 4 $a Physical chemistry. $3 1981412
650 4 $a Virology. $3 642304
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710 2 $a Indiana University. $b Chemistry. $3 1020748
773 0 $t Dissertation Abstracts International $g 78-07B(E).
790 $a 0093
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792 $a 2017
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10257038