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Enzyme-linked immunosorbent assay (E...

Hosseini, Samira.

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Enzyme-linked immunosorbent assay (ELISA) = from A to Z /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Enzyme-linked immunosorbent assay (ELISA)/ by Samira Hosseini ... [et al.].
Reminder of title:	from A to Z /
other author:	Hosseini, Samira.
Published:	Singapore :Springer Singapore : : 2018.,
Description:	xi, 115 p. :ill., digital ;24 cm.
[NT 15003449]:	1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.
Contained By:	Springer eBooks
Subject:	Enzyme-linked immunosorbent assay. -
Online resource:	http://dx.doi.org/10.1007/978-981-10-6766-2
ISBN:	9789811067662

Enzyme-linked immunosorbent assay (ELISA) = from A to Z /
Enzyme-linked immunosorbent assay (ELISA)from A to Z /[electronic resource] :by Samira Hosseini ... [et al.]. - Singapore :Springer Singapore :2018. - xi, 115 p. :ill., digital ;24 cm. - SpringerBriefs in applied sciences and technology,2191-530X. - SpringerBriefs in applied sciences and technology..

1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.

This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

ISBN: 9789811067662

Standard No.: 10.1007/978-981-10-6766-2doiSubjects--Topical Terms:

3299846
Enzyme-linked immunosorbent assay.

LC Class. No.: QP519.9.E48

Dewey Class. No.: 610.28

Enzyme-linked immunosorbent assay (ELISA) = from A to Z /
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245 0 0 $a Enzyme-linked immunosorbent assay (ELISA) $h [electronic resource] : $b from A to Z / $c by Samira Hosseini ... [et al.].
260 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2018.
300 $a xi, 115 p. : $b ill., digital ; $c 24 cm.
490 1 $a SpringerBriefs in applied sciences and technology, $x 2191-530X
505 0 $a 1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.
520 $a This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
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