東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Mechanisms of RNA sorting into exosomes.

Shurtleff, Matthew James.

FindBook

Google Book

Amazon

博客來

Mechanisms of RNA sorting into exosomes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Mechanisms of RNA sorting into exosomes./
作者:	Shurtleff, Matthew James.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2016,
面頁冊數:	72 p.
附註:	Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.
Contained By:	Dissertation Abstracts International78-06B(E).
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10192363
ISBN:	9781369558821

Mechanisms of RNA sorting into exosomes.
Shurtleff, Matthew James.

Mechanisms of RNA sorting into exosomes. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 72 p.

Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.

Thesis (Ph.D.)--University of California, Berkeley, 2016.

Exosomes are vesicles that are released by cells into the extracellular environment and populate all bodily fluids. These vesicles contain molecular cargo, including RNA, proteins and lipids and therefore may serve as vehicles for intercellular communication by transferring unconventional signals between cells. Despite widespread scientific interest in the physiological role of exosomes in health and disease, little is currently known about how molecules are selectively sorted into exosomes.

ISBN: 9781369558821Subjects--Topical Terms:

3172791
Cellular biology.

Mechanisms of RNA sorting into exosomes.
LDR:02951nmm a2200301 4500 001 2124019
005 20171023101704.5
008 180830s2016 ||||||||||||||||| ||eng d
020 $a 9781369558821
035 $a (MiAaPQ)AAI10192363
035 $a AAI10192363
040 $a MiAaPQ $c MiAaPQ
100 1 $a Shurtleff, Matthew James. $3 3285984
245 1 0 $a Mechanisms of RNA sorting into exosomes.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2016
300 $a 72 p.
500 $a Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.
500 $a Adviser: Randy W. Schekman.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2016.
520 $a Exosomes are vesicles that are released by cells into the extracellular environment and populate all bodily fluids. These vesicles contain molecular cargo, including RNA, proteins and lipids and therefore may serve as vehicles for intercellular communication by transferring unconventional signals between cells. Despite widespread scientific interest in the physiological role of exosomes in health and disease, little is currently known about how molecules are selectively sorted into exosomes.
520 $a In the work described herein, I used biochemical approaches to purify exosomes from cells grown in culture and identify microRNAs that are selectively sorted into exosomes. I then developed a cell-free reaction that reconstitutes the selective sorting of microRNA into exosomes in vitro. The reaction was then utilized to identify an RNA binding protein, Y-box Protein I (YBX1), that is required for sorting an exosomal microRNA. Next, I used the cell-free reaction as the basis for a selection strategy (termed Exo-SELEX) to identify primary RNA sequence motifs that act as positive and negative sorting signals in vitro. Finally, I used a high throughput RNA sequencing approach (TGIRT-seq) that allowed for a comprehensive transcriptome analysis (including highly structured or modified transcripts) of purified exosomes from normal and YBX1 knockout cells. TGIRT-seq analysis revealed that the most abundant transcript biotypes in exosomes are tRNA followed by other small non-coding RNA species. The abundant small non-coding RNA (tRNA, Y-RNA and Vault RNA) were strongly depleted in YBX1 knockout exosomes while sequences representing long non-coding RNA and protein coding genes were unaffected, indicating that these are sorted through a separate mechanism and allowing for broad classification of exosomal RNA based on YBX1-dependence or independence. In sum, this work provides a preliminary mechanistic understanding of the process of RNA sorting into exosomes and establishes multiple tools for the continued dissection of these pathways.
590 $a School code: 0028.
650 4 $a Cellular biology. $3 3172791
650 4 $a Biochemistry. $3 518028
690 $a 0379
690 $a 0487
710 2 $a University of California, Berkeley. $b Microbiology. $3 3193068
773 0 $t Dissertation Abstracts International $g 78-06B(E).
790 $a 0028
791 $a Ph.D.
792 $a 2016
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10192363